Regulation of the β₂‐adrenergic receptor and its mRNA in the rat ventral prostate by testosterone

Abstract

To investigate the regulation of fl2AR expression in rat ventral prostate the effects of castration and testosterone replacement on the fl2AR were studied by ligand binding and Northern blot analysis. Orchidectomy depressed fl2AR number by 50% within 4 days and testosterone administration to 4-day castrates produced a rapid and complete recovery offl2AR number within 24 h. In contrast to receptor number, fl2AR mRNA levels did not change relative to total RNA following castration. However, during the testosterone replacement period fl2AR mRNA levels rose transiently, reaching a maximum (3.5-fold) between 8 and 12 h, and this increase in mRNA preceded the recovery in B2AR number in the membrane. Regulation of fl2AR gene expression by testosterone in the ventral prostate is thus complex and probably involves both transcriptional and post-transcriptional components.fl2-Adrenergic receptor; Androgen; Ventral prostate; mRNA

Effects of cocaine administration on receptor binding and subunits mRNA of GABAA-benzodiazepine receptor complexes

“…mRNA transcription rate was noted after only 20 -30 min of treatment. Such a rapid change in mRNA transcription is mostly transient (Noguchi et al, 1982;Collins et al, 1988). In our acute experiments, we also found that changes in mRNA levels were small (15-25%) and transient (data not shown).…”

The Coming of Age of the Epididymis

“…In comparing the regulation of epididymal Sa-reductase enzyme activity and the expression of its mRNAs, it is apparent that another potential regulatory site for the enzyme is at the level of the protein itself. It is interesting to note that post-transcriptional regulation has also been proposed for several other epididymal proteins (Cornwall and Hann, 199Sb;Brooks et al, 1986;Cyr et al, 1993;Ghyselinck et al, 1990) and other proteins in the male reproductive tract (Kistler et al, 1981;Collins et al, 1988).…”

RU-486 (Mifepristone) ameliorates diabetes but does not correct deficient β-adrenergic signalling in adipocytes from mature C57BL/6J-ob/ob mice

“…The small but signi®cant reduction in b 2 -AR mRNA in RU-486-treated ob/ob supports this suggestion in part. Since the b 2 -AR is also positively regulated by testosterone, 50 the poor pituitary support for testicular function in ob/ob mice 34 may be counteracting the stimulatory effects of glucocorticoids on b 2 -AR expression. The effect of RU-486 on testosterone levels was not evaluated in the present study, but as with thyroid hormones, it seems unlikely that the antagonist would signi®cantly alter testicular function.…”