Regulation of tissue β-adrenergic, glucocorticoid and androgen receptors induced by repeated exposure to growth promoters in male veal calves

Odore, R.; Badino, P.; Barbero, Raffaella; Cuniberti, B.; Pagliasso, S.; Girardi, C.; Re, G.

doi:10.1016/j.rvsc.2006.12.011

Cited by 21 publications

(15 citation statements)

References 28 publications

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“…The dose-independent effect on heart weight and length could be partially due to a phenomenon of desensitization or down-regulation of cardiac beta-adrenoceptors. The down regulation of cardiac beta-adrenoceptors has in fact already been observed in other species such as cattle (Badino et al, 2008;Odore et al, 2007).Despite the fact that no statistically significant differences were observed in blood chemistry values among the four groups, all treated animals showed a certain degree of eosinophilic cell infiltration in the lymphoid organs. The increase in eosinophil circulating number and/or in tissue eosinophils, is associated with a wide variety of parasitic infestation, allergic responses, neoplasms, connective tissue disorders, medications and endocrinopathies (Bochsler and Slauson, 2002; Gotlib, 2005).…”

supporting

confidence: 52%

Section: Discussionmentioning

confidence: 74%

“…In cattle, reproductive system modifications have been described following treatment with steroid hormones (Biolatti et al, 2003). Moreover, prolonged clenbuterol exposure has been observed to induce pathological modifications of the female reproductive system, increased oestrogen and progesterone receptor concentrations, and a marked decrease in β-adrenoceptors in the heart, bronchial tubes and brain of veal calves (Odore et al, 2007;Re et al, 1995Re et al, , 1997. By contrast, Schiavone et al (2004) found a down-regulation of androgen receptors in the male reproductive system of broilers treated with repartitioning doses of clenbuterol.…”

mentioning

confidence: 99%

“…No pathological features are reported in the thyroid despite the decrease in thyroid hormone concentrations. The role of RAC in the determinism of eosinophilic infiltration in lymphoid organs and in the ovary of gilts remains to be clarified.Additional research on a larger group of pigs is needed to define the biomolecular mechanisms underlying the observed lesions.The study was supported by grants from the Regione Pie-monte, Italy (Progetti Ricerca Sanitaria Finalizzata 2006-2007. …”

mentioning

confidence: 99%

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Physiopathological changes related to the use of ractopamine in swine: Clinical and pathological investigations

Catalano¹,

Odore²,

Amedeo³

et al. 2012

Livestock Science

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The aim of the study was to investigate clinical and pathological modifications induced by treatment with different doses of the β2-agonist ractopamine (RAC) in pigs. Thirty-two eight-month-old LandracexLarge White pigs (mean weight, 102.8 ±6.7 kg) were treated with 0, 10 (R10), 20 (R20) and 40 (R40) mg/kg/diet of RAC for the last 35 days before slaughtering. Before and after treatment body weight was recorded, blood samples were collected for complete blood count and thyroid hormone concentration analysis, and cardiac instrumental evaluation was performed. At slaughtering, tissue samples were collected for histopathological examination. None of the animals showed any signs of clinical disease during the study period. Treatment did not induce any statistically significant difference in complete blood counts (P>0.05) among groups.A significant (Pb0.05) decrease in feed consumption was observed between R10 and R40 treated animals. A significant (Pb0.01) increase in ventricular internal dimensions and left ventricular mass was observed in R20; a significant reduction in thyroid weight (Pb0.05) was observed in the animals receiving the highest-dose treatment (R40). Urethral hyperplasia and metaplasia, and prostate hyperplasia and a significant (Pb0.05) reduction in circulating triiodothyronine levels were found in overall treated animals. Taken together, these data suggest that RAC treatment in pigs induces adaptative non-neoplastic urethral and prostatic disorders of growth and a reduction in thyroid hormone concentrations. Nevertheless, additional research on a larger group of animals is needed to define the biomolecular mechanisms underlying the observed lesions, in order to improve the surveillance tools to identify illegal treatments. IntroductionRactopamine (RAC) is a ß-adrenergic agonist (ß-agonist) which at repartitioning doses has been found to increase muscle growth and decrease fat deposition in different animal species (Baker et al., 1984;Mersmann, 1998;Mills and Spurlock, 2003;Ricks et al., 1984;Sillence, 2004;Watkins et al., 1990). In pigs and cattle treated during the final period of the finishing improved feed efficiency, heavier carcass weights, and higher dressing percentages have been documented (Gu et al., 1991a,b; Laudert et al., 2005a,b; Poletto et al., 2009;See et al., 2004).The dosage required to induce "anabolic" effects is generally 10-20 times higher than that indicated for therapeutic purposes. At elevated doses, certain β2-agonists such as clenbuterol can cause morphological, histological and/or biochemical changes in various organs of exposed animals (Biolatti et al., 1994;Sillence et al., 1993). In vivo, β-adrenergic agonists may induce secondary events caused by hormonal or physiological responses of several tissues, involving especially endocrine and cardio-respiratory organs.In pigs, treatment during the finishing with repartitioning doses of β-agonists has been associated with modifications of vascular microcirculation characterized by ulcerative lesions of the distal extremities...

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supporting

confidence: 52%

Section: Discussionmentioning

confidence: 74%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Physiopathological changes related to the use of ractopamine in swine: Clinical and pathological investigations

Catalano¹,

Odore²,

Amedeo³

et al. 2012

Livestock Science

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“…Mersmann (1998) found that PAA caused alteration in testicular blood flow and a consequent change i n testicular temperature which might have affected sperm motility. Furthermore, Odore (2007) found lighter testicles when they include PAA in veal calves. However, in the study, testicular temperature was not examined.…”

Section: -30 G D-'mentioning

confidence: 90%

Zilpaterol Hydrochloride on Performance and Sperm Quality of Lambs Fed Wet Brewers Grains

Aguilera-Soto¹,

Ramirez²,

Aréchiga³

et al. 2008

Journal of Applied Animal Research

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Recombinant cell bioassays for the detection of (gluco)corticosteroids and endocrine-disrupting potencies of several environmental PCB contaminants

et al. 2011

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Sensitive and robust bioassays for glucocorticoids are very useful for the pharmaceutical industry, environmental scientists and veterinary control. Here, a recombinant yeast cell was constructed that expresses the human glucocorticoid receptor alpha and a green fluorescent reporter protein in response to glucocorticoids. Both the receptor construct and the reporter construct were stably integrated into the yeast genome. The correct and specific functioning of this yeast glucocorticoid bioassay was studied by exposures to cortisol and other related compounds and critically compared to a GR-CALUX bioassay based on a human bone cell. Although less sensitive, the new yeast glucocorticoid bioassay showed sensitivity towards all (gluco)corticoids tested, with the following order in relative potencies: budesonide >> corticosterone > dexamethasone > cortisol = betamethasone > prednisolone > aldosterone. Hormone representatives for other hormone nuclear receptors, like 17β-estradiol for the oestrogen receptor, 5α-dihydrotestosterone for the androgen receptor and progesterone for the progesterone receptor, showed no clear agonistic responses, whilst some polychlorinated biphenyls were clearly able to interfere with the GR activity.

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Regulation of tissue β-adrenergic, glucocorticoid and androgen receptors induced by repeated exposure to growth promoters in male veal calves

Cited by 21 publications

References 28 publications

Physiopathological changes related to the use of ractopamine in swine: Clinical and pathological investigations

Physiopathological changes related to the use of ractopamine in swine: Clinical and pathological investigations

Zilpaterol Hydrochloride on Performance and Sperm Quality of Lambs Fed Wet Brewers Grains

Recombinant cell bioassays for the detection of (gluco)corticosteroids and endocrine-disrupting potencies of several environmental PCB contaminants

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