Regulation of transcription and activity of Rhizobium etli glutaminase A

Huerta-Saquero, Alejandro; Calderón-Flores, Arturo; Dı́az-Villaseñor, Andrea; Pont, Gisela Du; Durán, Socorro

doi:10.1016/j.bbagen.2004.05.001

Biochimica et Biophysica Acta (BBA) - General Subjects

2004

DOI: 10.1016/j.bbagen.2004.05.001

|View full text |Cite

Regulation of transcription and activity of Rhizobium etli glutaminase A

Alejandro Huerta-Saquero¹,

Arturo Calderón-Flores²,

Andrea Dı́az-Villaseñor³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2009

2014

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Glutathione Is Required by Rhizobium etli for Glutamine Utilization and Symbiotic Effectiveness

Tatè¹,

Cermola²,

Riccio³

et al. 2012

MPMI

View full text Add to dashboard Cite

Here, we provide genetic and biochemical evidence indicating that the ability of Rhizobium etli bacteria to efficiently catabolize glutamine depends on its ability to produce reduced glutathione (l-γ-glutamyl-l-cysteinylglycine [GSH]). We find that GSH-deficient strains, namely a gshB (GSH synthetase) and a gor (GSH reductase) mutant, can use different amino acids, including histidine, alanine, and asparagine but not glutamine, as sole source of carbon, energy, and nitrogen. Moreover, l-buthionine(S,R)-sulfoximine, a GSH synthesis inhibitor, or diamide that oxidizes GSH, induced the same phenotype in the wild-type strain. Among the steps required for its utilization, glutamine uptake, occurring through the two well-characterized carriers (Aap and Bra systems) but not glutamine degradation or respiration, was largely reduced in GSH-deficient strains. Furthermore, GSH-deficient mutants of R. etli showed a reduced symbiotic efficiency. Exogenous GSH was sufficient to rescue glutamine uptake or degradation ability, as well as the symbiotic effectiveness of GSH mutants. Our results suggest a previously unknown GSH-glutamine metabolic relationship in bacteria.

show abstract

Glutathione Is Required by Rhizobium etli for Glutamine Utilization and Symbiotic Effectiveness

Tatè¹,

Cermola²,

Riccio³

et al. 2012

MPMI

View full text Add to dashboard Cite

show abstract

Key roles of microsymbiont amino acid metabolism in rhizobia-legume interactions

Dunn¹

2014

Critical Reviews in Microbiology

View full text Add to dashboard Cite

Rhizobia are bacteria in the α-proteobacterial genera Rhizobium, Sinorhizobium, Mesorhizobium, Azorhizobium and Bradyrhizobium that reduce (fix) atmospheric nitrogen in symbiotic association with a compatible host plant. In free-living and/or symbiotically associated rhizobia, amino acids may, in addition to their incorporation into proteins, serve as carbon, nitrogen or sulfur sources, signals of cellular nitrogen status and precursors of important metabolites. Depending on the rhizobia-host plant combination, microsymbiont amino acid metabolism (biosynthesis, transport and/or degradation) is often crucial to the establishment and maintenance of an effective nitrogen-fixing symbiosis and is intimately interconnected with the metabolism of the plant. This review summarizes past findings and current research directions in rhizobial amino acid metabolism and evaluates the genetic, biochemical and genome expression studies from which these are derived. Specific sections deal with the regulation of rhizobial amino acid metabolism, amino acid transport, and finally the symbiotic roles of individual amino acids in different plant-rhizobia combinations.

show abstract

L-Glutaminase Production and the Growth of Marine Bacteria

Praka¹,

Poorani²,

Anantharam³

et al. 2009

Research J. of Microbiology

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Regulation of transcription and activity of Rhizobium etli glutaminase A

Cited by 3 publications

References 32 publications

Glutathione Is Required by Rhizobium etli for Glutamine Utilization and Symbiotic Effectiveness

Glutathione Is Required by Rhizobium etli for Glutamine Utilization and Symbiotic Effectiveness

Key roles of microsymbiont amino acid metabolism in rhizobia-legume interactions

L-Glutaminase Production and the Growth of Marine Bacteria

Contact Info

Product

Resources

About