Regulation of Two Photosynthetic Pigment-related Genes During Stress-induced Pigment Formation in the Green Alga, Dunaliella salina

Sánchez-Estudillo, Leticia; Freile‐Pelegrín, Yolanda; Rivera‐Madrid, Renata; Robledo, Daniel; Narváez-Zapata, José Alberto

doi:10.1007/s10529-006-9001-2

Cited by 35 publications

(22 citation statements)

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“…3). Carotene and chlorophyll a balance in DUNS-2 under saline stress conditions are in agreement with those reported in other productive Dunaliella isolates (Sánchez-Estudillo et al 2006;Ramos et al 2008), suggesting a carotenogenic potential of this Dunaliella isolate.…”

Section: Resultssupporting

confidence: 89%

“…D. salina has the potential to overcome these difficulties and is widely employed for the production of valuable fine chemicals such as carotenes (Ben-Amotz et al 1991). This species has developed several physiological adaptations including the lack of a rigid cell wall, a variable intracellular concentration of glycerol (Kaçka and Dönmez 2008), changes in photosynthetic pigments balance (Sánchez-Estudillo et al 2006) and structural modifications in the chloroplast (Stonynova-Bakalova and Toncheva-Panova 2003). These, physiological variations under stress condition have hindered the identification of this genus, mainly when different carotenogenic strains are analyzed.…”

Section: Introductionmentioning

confidence: 99%

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Different physiological responses influenced by salinity in genetically related Dunaliella salina isolates

et al. 2011

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An isolate of Dunaliella salina (DUNS-1) and other two isolates (DUNS-2 and DUNS-3), collected from coastal lagoons with 14 and 30% (w/v) of NaCl, respectively, were analyzed under different saline conditions. Glycerol (380 mg l(-1)) and carotene (5.9 mg l(-1)) contents for DUNS-2 were 0.3 and 10 times higher than DUNS-3, even though both isolates were collected from the same lagoon and share a similar ribosomal DNA sequence.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Different physiological responses influenced by salinity in genetically related Dunaliella salina isolates

et al. 2011

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“…Some of Dunaliella strains are well known for the high production of carotenoids, especially β-carotene ). This alga produces large amount of β-carotene under stress conditions such as high light (HL) (Vorst et al 1994, Coesel et al 2008, Lamers et al 2010, high salinity (Vorst et al 1994, Hadi et al 2008, nutrient limitation (Marín et al 1998, Coesel et al 2008, and nitrogen starvation (Sánchez-Estudillo et al 2006, Lamers et al 2012. Ability of Dunaliella to thrive under extreme salinities gives a selective advantage that inhibits growth of other algae and its predators.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the responses of two Dunaliella strains, Dunaliella salina CCAP 19/18 and Dunaliella bardawil to light intensity with special emphasis on carotenogenesis

Park¹,

Lee²,

Jin³

2013

ALGAE

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Dunaliella salina and Dunaliella bardawil are well known for carotenogenesis, the overproduction of carotenoids, under stress conditions. The effect of high light (HL) and low light (LL) on the growth, morphology, photosynthetic efficiency, and the β-carotene and zeaxanthin production of D. salina CCAP 19/18 and D. bardawil was investigated and compared. Both strains showed similar growth kinetics under LL growth condition, but D. salina CCAP 19/18 was faster. As the light intensity increased, D. salina CCAP 19/18 cells were elongated and D. bardawil cells became larger. Both strains showed decrease of the maximum quantum yield of PSII (F v /F m ) and election transport rate (ETR) under HL growth condition and D. salina CCAP 19/18 was less liable to the light stress. Both strains had about 1.8 and 5 times difference in the O 2 evolution rate at LL and HL conditions, respectively. The β-carotene and zeaxanthin production were increased as the light intensity increased in both strains. D. bardawil was more sensitive to light intensity than D. salina CCAP 19/18. The possible application of D. salina CCAP 19/18 as a carotenogenic strain will be discussed.

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“…Despite the knowledge that high-light-induced b-carotene overproduction requires both transcriptional and translational activity (Lers et al, 1990), it remains unclear what genes and enzymes are involved in the regulation of the overproduction. Various studies (Coesel et al, 2008;Rabbani et al, 1998;Ramos et al, 2007Ramos et al, , 2009Sanchez-Estudillo et al, 2006) have been directed towards elucidating the role of structural genes that encode enzymes of the carotenoid biosynthetic pathway, but an unambiguous consensus is still lacking (Lamers et al, 2008). Alternative regulatory mechanisms have been proposed as well, including the hypothesis that b-carotene overproduction is driven by the formation of lipid globules (Rabbani et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Carotenoid and fatty acid metabolism in light‐stressed Dunaliella salina

Lamers

Laak

Kaasenbrood

et al. 2010

Biotech & Bioengineering

239

127

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beta-Carotene is overproduced in the alga Dunaliella salina in response to high light intensities. We have studied the effects of a sudden light increase on carotenoid and fatty acid metabolism using a flat panel photobioreactor that was run in turbidostat mode to ensure a constant light regime throughout the experiments. Upon the shift to an increased light intensity, beta-carotene production commenced immediately. The first 4 h after induction were marked by constant intracellular levels of beta-carotene (2.2 g LCV(-1)), which resulted from identical increases in the production rates of cell volume and beta-carotene. Following this initial phase, beta-carotene productivity continued to increase while the cell volume productivity dropped. As a result, the intracellular beta-carotene concentration increased reaching a maximum of 17 g LCV(-1) after 2 days of light stress. Approximately 1 day before that, the maximum beta-carotene productivity of 30 pg cell(-1) day(-1) (equivalent to 37 mg LRV(-1) day(-1)) was obtained, which was about one order of magnitude larger than the average productivity reported for a commercial beta-carotene production facility, indicating a vast potential for improvement. Furthermore, by studying the light-induced changes in both beta-carotene and fatty acid metabolism, it appeared that carotenoid overproduction was associated with oil globule formation and a decrease in the degree of fatty acid unsaturation. Our results indicate that cellular beta-carotene accumulation in D. salina correlates with accumulation of specific fatty acid species (C16:0 and C18:1) rather than with total fatty acid content.

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Regulation of Two Photosynthetic Pigment-related Genes During Stress-induced Pigment Formation in the Green Alga, Dunaliella salina

Cited by 35 publications

References 14 publications

Different physiological responses influenced by salinity in genetically related Dunaliella salina isolates

Different physiological responses influenced by salinity in genetically related Dunaliella salina isolates

Comparison of the responses of two Dunaliella strains, Dunaliella salina CCAP 19/18 and Dunaliella bardawil to light intensity with special emphasis on carotenogenesis

Carotenoid and fatty acid metabolism in light‐stressed Dunaliella salina

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