Regulation of Vascular Endothelial Growth Factor Synthesis and Release by Human Luteal Cells<i>in Vitro</i>

Tropea, Anna; Miceli, Fiorella; Minici, Francesca; Tiberi, Federica; Orlando, Mariateresa; Gangalè, Maria Francesca; Romani, Federica; Catino, Stefania; Mancuso, Stefano; Navarra, Pierluigi; Lanzone, Antonio; Apa, Rosanna

doi:10.1210/jc.2005-2457

Cited by 37 publications

(31 citation statements)

References 45 publications

(55 reference statements)

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“…These unexpected data could be partially explained by our observation of an Es inhibitory effect on luteal P production. Indeed we previously demonstrated the ability of P to decrease VEGF luteal secretion and expression (13). Based on these data it is tempting to speculate that after an Es-mediated P decrease, the lack of any P inhibitory effect on VEGF production could contribute to increase luteal VEGF release.…”

Section: Estrogens and Vegf In Human Lcmentioning

confidence: 86%

“…To analyze VEGF messenger RNA (mRNA) modulation in human LC, attention has been focused on diffusible mRNA expression of the VEGF 121 and VEGF 165 isoforms (13). The latter was evaluated by real-time RT-PCR using the i-Cycler iQ system (Bio-Rad Laboratories, Hercules, CA), as previously described (22).…”

Section: Total Rna Extraction Cdna Synthesis and Real-time Pcr Analmentioning

confidence: 99%

“…Indeed PGF 2a seems to be involved in human CL regression (10,11), conversely for PGE 2 , a luteotropic role has been suggested (11,12). In addition, given the essential role of vascular endothelial growth factor (VEGF) in human CL maintenance (13,14), the possible effect of Es on VEGF luteal secretion and expression was investigated.…”

mentioning

confidence: 99%

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Estrogens and androgens affect human luteal cell function

Tropea

Lanzone

Tiberi³

et al. 2010

Fertility and Sterility

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Section: Estrogens and Vegf In Human Lcmentioning

confidence: 86%

Section: Total Rna Extraction Cdna Synthesis and Real-time Pcr Analmentioning

confidence: 99%

See 1 more Smart Citation

Estrogens and androgens affect human luteal cell function

Tropea

Lanzone

Tiberi³

et al. 2010

Fertility and Sterility

Self Cite

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“…VEGF is considered to be critical in the regulation of normal and abnormal angiogenesis in the ovary (3,9,(13)(14)(15)(16)(17)25,30), particularly in the newly formed corpus luteum. In primates, VEGF protein is localized in the hormone-producing cells of the corpus luteum, and is highest in the granulosa-derived cells (14)(15)(16)(30)(31)(32)(33)(34)(35). VEGF is fundamental in the physiological angiogenesis and vascularization of the follicular luteinizing granulosa layer during corpus luteum formation (15,16,30,32).…”

Section: Discussionmentioning

confidence: 99%

Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats

Zhang

Pan

et al. 2015

Mol Med Report

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Abstract. Vascular endothelial growth factor (VEGF) is vital in normal and abnormal angiogenesis in the ovary, particularly during the early development of the corpus luteum in the ovary. However, the molecular regulation of the expression VEGF during luteal development in vivo remains to be fully elucidated. As the expression of VEGF is mediated by hypoxia-inducible factor (HIF)-1α in luteal cells cultured in vitro, determined in our previous study, the present study was performed to confirm the hypothesis that HIF-1α is induced and then regulates the expression of VEGF and VEGF-dependent luteal development/function in vivo. This was investigated using a pregnant rat model treated with a small-molecule inhibitor of HIF-1α, echinomycin (Ech). The development of the corpus luteum in the pregnant rat ovary was identified via performing assays of the serum progesterone, testosterone and estradiol concentrations by radioimmunoassay, accompanied with determination of the changes in the expression levels of HIF-1α and VEGF by reverse transcription-quantitative polymerase chain reaction at different days of the developmental process. On day 5, serum progesterone levels were markedly increased, whereas serum levels of testosterone and estradiol did not change significantly. On day 17, the highest level of serum progesterone was observed, however, this was not the case for testosterone and estradiol. Further analysis of the expression levels of HIF-1α and VEGF revealed that their changes were consistent with the changes in serum levels of progesterone, which occurred in the development of the corpus luteum in the ovaries of pregnant rats. Further investigation demonstrated that Ech inhibited luteal development through inhibiting the expression of VEGF, mediated by HIF-1α, and subsequent luteal function, which was determined by detecting changes in serum progesterone on days 8 and 14. Taken together, these results demonstrated that HIF-1α-mediated expression of VEGF may be one of the important mechanisms regulating ovarian luteal development in mammals in vivo, which may provide novel strategies in treatment for fertility control and for certain types of ovarian dysfunction, including polycystic ovarian syndrome, ovarian hyperstimulation syndrome and ovarian neoplasia.

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“…A cohort of oocytes begins to grow and develop in the ovary each month. Hypoxia of the granulosa cells is a normal event during the growth of ovarian follicles [30]. Hypoxic or anoxic conditions reduce ROS and increase antioxidant activity [31].…”

Section: Oxidative Stress and The Ovarymentioning

confidence: 99%