Regulation of Vegetative Phase Change by SWI2/SNF2 Chromatin Remodeling ATPase BRAHMA

Xu, Yunmin; Guo, Changkui; Zhou, Bingying; Li, Chenlong; Wang, Huaseng; Zheng, Bentuo; Ding, Han‐Fei; Zhu, Zhujun; Perucatti, A.; Cui, Yuhai; Poethig, R. Scott; Wu, Gang

doi:10.1104/pp.16.01588

Cited by 78 publications

(80 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To test whether this H3K27me3 modification plays a role in anthocyanin biosynthesis, we characterized the phenotype of two mutants carrying null alleles of SWN and CLF, swn-3 and clf-29, which showed reduced H3K27me3 levels Wang et al, 2016;Xu et al, 2016). We also analyzed the phenotype of the PRC1 subunit TERMINAL FLOWER2 (TFL2) overexpression line, TFL2-OX, which showed increased H3K27me3 levels (Liu et al, 2014).…”

Section: The Altered H3k27me3 Level Is Not Associated With Changes Inmentioning

confidence: 99%

Epigenetic regulation of anthocyanin biosynthesis by an antagonistic interaction between H2A.Z and H3K4me3

et al. 2018

View full text Add to dashboard Cite

The accumulation of anthocyanins in response to specific developmental cues or environmental conditions plays a vital role in plant development and protection against stresses. Extensive research has examined the regulation of anthocyanin biosynthetic genes at the transcriptional and post-transcriptional levels, but the role of chromatin in this regulation remains unknown. Chromatin immunoprecipitation and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses were performed. Genetic interactions between trimethylation of lysine 4 on histone H3 (H3K4me3) and the chromatin remodeling complex SWR1 in the control of anthocyanin biosynthesis were further studied. In this study, we provide evidence that a conserved histone H2 variant, H2A.Z, negatively regulates anthocyanin accumulation through deposition at a set of anthocyanin biosynthetic genes and consequently represses their expression in Arabidopsis thaliana. Our data indicate that the accumulation of anthocyanin in H2A.Z deposition-deficient mutants is associated with increased H3K4me3, which is required for promotion of the expression of anthocyanin biosynthetic genes. We further provide evidence that H3K4me3 in anthocyanin biosynthetic genes is negatively associated with the presence of H2A.Z. Our results reveal an antagonistic relationship between H2A.Z and H3K4me3 in the regulation of the expression of anthocyanin biosynthesis genes, adding another layer of regulation to anthocyanin biosynthesis genes and highlighting the role of chromatin in gene regulation.

show abstract

Section: The Altered H3k27me3 Level Is Not Associated With Changes Inmentioning

confidence: 99%

Epigenetic regulation of anthocyanin biosynthesis by an antagonistic interaction between H2A.Z and H3K4me3

et al. 2018

View full text Add to dashboard Cite

show abstract

“…CLF is also necessary for WUSCHEL (WUS) repression, contributing to the termination of floral stem cells (Liu et al, 2011), and is required for maintaining root meristem activity by antagonizing the chromatin remodeler PICKLE (PKL) (Aichinger, Villar, Di Mambro, Sabatini, & Köhler, 2011). Unlike the clf mutants that display severe phenotypes including dwarfism, curly leaf, and early flowering, the swn mutants only show weak changes during vegetative phase transition (Xu, Hu, Smith, & Poethig, 2016;Xu, Guo, et al, 2016). However, the clf swn double mutants lose the capacity to differentiate and form massive somatic embryo-like structures (Chanvivattana et al, 2004;Farrona et al, 2011;Lu, Cui, Zhang, Jenuwein, & Cao, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings

et al. 2019

Self Cite

View full text Add to dashboard Cite

The Polycomb Group (PcG) proteins form two protein complexes, PcG Repressive Complex 1 ( PRC 1) and PRC 2, which are key epigenetic regulators in eukaryotes. PRC 2 represses gene expression by catalyzing the trimethylation of histone H3 lysine 27 (H3K27me3). In Arabidopsis ( Arabidopsis thaliana ), CURLY LEAF ( CLF ) and SWINGER ( SWN ) are two major H3K27 methyltransferases and core components of PRC 2, playing essential roles in plant growth and development. Despite their importance, genome‐wide binding profiles of CLF and SWN have not been determined and compared yet. In this study, we generated transgenic lines expressing GFP ‐tagged CLF / SWN under their respective native promoters and used them for Ch IP ‐seq analyses to profile the genome‐wide distributions of CLF and SWN in Arabidopsis seedlings. We also profiled and compared the global H3K27me3 levels in wild‐type ( WT ) and PcG mutants ( clf , swn , and clf swn ). Our data show that CLF and SWN bind to almost the same set of genes, except that SWN has a few hundred more targets. Two short DNA sequences, the GAGA ‐like and Telo ‐box‐like motifs, were found enriched in the CLF and SWN binding regions. The H3K27me3 levels in clf , but not in swn , were markedly reduced compared with WT ; and the mark was undetectable in the clf swn double mutant. Further, we profiled the transcriptomes in clf , swn , and clf swn, and compared that with WT . Thus this work provides a useful resource for the plant epigenetics community for dissecting the functions of PRC 2 in plant growth and development.

show abstract

“…miR156 and SPL9/13/15 are all expressed throughout the shoot apex in both the shoot apical meristem (SAM) and young leaf primordia (6). While there has been recent progress in elucidating the molecular mechanisms that control the temporal decline of miR156 expression (8)(9)(10)(11)(12)(13), how expression of the miR156-SPL pathway and the process of VPC are coordinated across the shoot apex remains unknown. It has long been thought that the identity of a shoot is determined by the maturation state of the SAM (14,15).…”

mentioning

confidence: 99%

Role for the shoot apical meristem in the specification of juvenile leaf identity inArabidopsis

Fouracre

Poethig

2019

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

The extent to which the shoot apical meristem (SAM) controls developmental decisions, rather than interpreting them, is a longstanding issue in plant development. Previous work suggests that vegetative phase change is regulated by signals intrinsic and extrinsic to the SAM, but the relative importance of these signals for this process is unknown. We investigated this question by examining the effect of meristem-deficient mutations on vegetative phase change and on the expression of key regulators of this process, miR156 and its targets, SPL transcription factors. We found that the precocious phenotypes of meristem-deficient mutants are a consequence of reduced miR156 accumulation. Tissue-specific manipulation of miR156 levels revealed that the SAM functions as an essential pool of miR156 early in shoot development, but that its effect on leaf identity declines with age. We also found that SPL genes control meristem size by repressing WUSCHEL expression via a novel genetic pathway.

show abstract

Regulation of Vegetative Phase Change by SWI2/SNF2 Chromatin Remodeling ATPase BRAHMA

Cited by 78 publications

References 64 publications

Epigenetic regulation of anthocyanin biosynthesis by an antagonistic interaction between H2A.Z and H3K4me3

Epigenetic regulation of anthocyanin biosynthesis by an antagonistic interaction between H2A.Z and H3K4me3

Genome‐wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings

Role for the shoot apical meristem in the specification of juvenile leaf identity inArabidopsis

Contact Info

Product

Resources

About