Regulation of Voltage Sensing Structures of CaV1.2 Calcium Channels by the Auxiliary β-Subunit (β3)

Abstract

channels which dramatically impairs stable recordings and thus reliable pharmacological assessment, especially in the study of slow or use-dependent compounds. Nanion's SyncroPatch 384PE offers a high throughput gigaseal platform which records from up to 384/768 experiments at the same time. It does not only allow for high quality recordings with reliable pharmacology, but also for a more detailed biophyiscal characterization of an ion channel protein. A temperature control feature supports measurements at physiological temperatures. Our results show very stable recordings of CaV1.2 over an extended period of time (> 25 min.) that permits for cumulative compound application. Furthermore, we demonstrate CaV1.2 activation from different states that discriminates state-and use-dependent drug effects. Our assay development demonstrates how accurate pharmacology and high-throughput recordings of even difficult targets like CaV1.2 can be achieved in a reproducible and reliable manner with the SyncroPatch 384PE. 2194-Pos Board B338Eminence of VSD I in the Voltage-Dependent Inactivation of the Human Ca V 1.2 Channel