Regulation of Volumes of the Muscle, Liver, and Brain Erythrocytes in Crucian Carp Carassius auratus (Cyprinidae) in Response to Increase in the Osmotic Concentration in Blood Plasma

Martemyanov, V. I.; Vasiliev, A. S.

doi:10.1134/s0032945218040112

Cited by 7 publications

(8 citation statements)

References 29 publications

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“…The critical salinity studied in the present paper is within the habitable salinity range of water for the freshwater teleosts. The living of goldfi sh in saline estuarine sections of rivers and the absence of mortality in experiments on fi sh acclimation to CS evidence the effectiveness of the homeostatic mechanisms of fi sh in CS conditions (21,25,26). This was also confi rmed by the results of the present study.…”

Section: Comparison Of Water-salt Homeostasis Parameters In the Body ...supporting

confidence: 86%

“…The sampling protocol for the tissues of liver, skeletal muscles, brain, erythrocytes and CSF was described in detail in (21).…”

Section: Methodsmentioning

confidence: 99%

“…Goldfi sh tolerates water salinity to 6.0 g/L without pronounced signs of stress (29). At water salinity in the range of 8.0-10.0 g/L and up to the upper limit of tolerable salinity (CS), the concentration of Na + in the plasma of goldfi sh and other freshwater Teleostei rises from ~ 150 to 200 mmol/kg, the level of cortisol in the blood is elevated, signs of muscle dehydration appear, along with a decreased locomotor activity and growth rates, decreased urine volume, and increased water consumption (18,19,20,21,27,28,29,30,31,32,33).…”

Section: Comparison Of Water-salt Homeostasis Parameters In the Body ...mentioning

confidence: 99%

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Goldfish as a model for studying the effect of hypernatremia on blood plasma lipoproteins

Андреева¹,

Martemyanov²,

Ilya³

et al. 2022

BLL

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AIM: To evaluate the effect of hypernatremia on the organization of blood plasma high density lipoproteins (HDL) in goldfi sh; to compare the state of hypernatremia in fi sh and humans; to assess the possible risks and consequences of the effect of hypernatremia on human plasma lipoproteins. METHODS: The fi sh were acclimated for 20 days at a critical salinity of 11.5 g/L; after that the salt water was gradually "desalinated". The concentration of Na + and the content of total water were determined in tissues, cells, and body fl uids. The HDL organization was assessed by the number of apolipoprotein molecules per particle. The methods of fl ame spectrophotometry, electrophoresis and MALDI were used. RESULTS: In fresh water, the state of normonatremia was maintained in the fi sh body; at critical water salinity, the state of hypernatremia. Against the background of hypernatremia, the initial signs of muscle and erythrocyte dehydration appeared in fi sh, the total water content in the plasma did not change, and HDL disintegrated into small particles, which, upon restoration of normonatremia, were combined into the original large forms. CONCLUSION: In goldfi sh at the state of normonatremia, large forms of HDL are stable while at the state of hypernatremia, the small forms of HDL are stable. Under conditions of a hypertonic environment and plasma hypernatremia, the breakdown of HDL prevents the loss of water from the fi sh organism and reduces the threat of their dehydration. Human hypernatremia is characterized by plasma sodium levels comparable to that in goldfi sh, however accompanied by life-threatening metabolic changes. The results of this study may be useful for assessing the risks of HDL breakdown at hypernatremia and for the development of protocols for the treatment of pathological conditions in humans (Fig. 4, Ref. 45).

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Section: Comparison Of Water-salt Homeostasis Parameters In the Body ...supporting

confidence: 86%

“…The sampling protocol for the tissues of liver, skeletal muscles, brain, erythrocytes and CSF was described in detail in (21).…”

Section: Methodsmentioning

confidence: 99%

Section: Comparison Of Water-salt Homeostasis Parameters In the Body ...mentioning

confidence: 99%

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Goldfish as a model for studying the effect of hypernatremia on blood plasma lipoproteins

Андреева¹,

Martemyanov²,

Ilya³

et al. 2022

BLL

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“…The osmotic concentration of blood plasma and muscle tissue of the body are in equilibrium (21). In the state of hypernatremia, the content of ions in the blood plasma of carp increased: sodium by 72 mmol/L, and calcium by 1.6 mmol/L, in total amounting to 73.6 mmol/L.…”

Section: Discussionmentioning

confidence: 99%

“…Muscle tissue was sampled in the zone of the 2nd-4th ribs. The subse-quent procedures of blood and muscle sampling were performed as described previously (21).…”

Section: Methodsmentioning

confidence: 99%

Volume regulation of muscle cells in the carp Cyprinus carpio in response to hypernatremia

Martemyanov¹,

Poddubnaya²

2019

BLL

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BACKGROUND: Hypernatremia supports the movement of water from the intracellular to the intercellular space. This shift leads to cells shrinkage and disruption of intracellular processes, representing risk factors of morbidity and mortality in clinical circumstances. On the other hand, hypernatremia triggers protective mechanisms that counteract damage of cells. OBJECTIVES: To determine in experiments in vivo the ranges of sodium content regulation in the blood plasma of carp characterizing the norm and hypernatremia. To identify the patterns volume regulation of skeletal muscles cells in response to hypernatremia. METHODS: Carps were acclimating for 3 weeks to a different salinity in the range of 0-12 g/L. In the plasma and muscle tissue the concentration of sodium, potassium, calcium and magnesium was determined by the method of fl ame spectrophotometry. Water content in the muscles has been additionally determined. RESULTS: Carps acclimated in the salinity range of 0-6 g/L, maintained the concentration of sodium in blood plasma within of the range of 129-135 mmol/L (normonatremia). In the salinity zone of 6-12 g/L concentration of sodium in the blood plasma of fi sh has increased to 207 mmol/L (hypernatremia). Hypernatremia was causing the increase of the sodium, potassium, calcium and magnesium content in carp muscles and the drop of water level. CONCLUSIONS: Muscle tissue of carp adapts to hypernatremia by means of increasing inorganic ions by 70.8 % and organic osmolytes by 29.2 % (Fig. 2, Ref. 51).

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High‐density lipoprotein remodeling affects the osmotic properties of plasma in goldfish under critical salinity

Andreeva,

Lamash,

Martemyanov

et al. 2023

Journal of Fish Biology

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To investigate the stress response and physiological adaptations of goldfish (Carassius auratus) to critical salinity (CS) waters, we analyzed high‐density lipoprotein (HDL) stoichiometry, stress markers (cortisol, glucose), and plasma osmotic properties (Na+, osmolality, water content) using ichthyology, biochemistry, and proteomics approaches. After 21 days of exposure to CS, plasma concentrations of cortisol, glucose, and Na+ increased, indicating stress. Total plasma osmolality (Osmtotal) and osmolality generated by inorganic (Osminorg) and organic osmolytes (Osmorg) also increased, the latter by ~2%. We associated the increase of Osmorg with (1) increased metabolite concentration (glucose), (2) dissociation of HDL particles resulting in increased HDL number per unit plasma volume (~1.5–2‐fold) and (3) increased HDL osmotic activity. HDL remodeling may be the reason for the redistribution of bound and free water in plasma, which may contribute to water retention in plasma and, at the same time, to hemodynamic disturbances under CS conditions. The study's findings suggest that HDL remodeling is an important mechanism for maintaining osmotic homeostasis in fish, which is consistent with current capillary exchange models in vertebrates.

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Regulation of Volumes of the Muscle, Liver, and Brain Erythrocytes in Crucian Carp Carassius auratus (Cyprinidae) in Response to Increase in the Osmotic Concentration in Blood Plasma

Cited by 7 publications

References 29 publications

Goldfish as a model for studying the effect of hypernatremia on blood plasma lipoproteins

Goldfish as a model for studying the effect of hypernatremia on blood plasma lipoproteins

Volume regulation of muscle cells in the carp Cyprinus carpio in response to hypernatremia

High‐density lipoprotein remodeling affects the osmotic properties of plasma in goldfish under critical salinity

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