2014
DOI: 10.3103/s0891416814020037
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Regulation of Yersinia pseudotuberculosis major porin expression in response to antibiotic stress

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Cited by 5 publications
(6 citation statements)
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“…The recombinant plasmids pACYC184-F-GFP and pACYC184-C-GFP containing the Y. pseudotuberculosis ompF and ompC regulatory regions fused to GFP were constructed as previously described [ 7 ]. To obtain the coding region of GFP, the plasmid DNA pTurboGFP (Evrogen, Moscow, Russia) was digested with Bam HI and Hind III (Fermentas, Waltham, MA, USA), and the resulting gfp fragment was dephosphorylated with CAP alkaline phosphatase (Fermentas, Waltham, MA, USA).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The recombinant plasmids pACYC184-F-GFP and pACYC184-C-GFP containing the Y. pseudotuberculosis ompF and ompC regulatory regions fused to GFP were constructed as previously described [ 7 ]. To obtain the coding region of GFP, the plasmid DNA pTurboGFP (Evrogen, Moscow, Russia) was digested with Bam HI and Hind III (Fermentas, Waltham, MA, USA), and the resulting gfp fragment was dephosphorylated with CAP alkaline phosphatase (Fermentas, Waltham, MA, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Y. pseudotuberculosis produces two major nonspecific porins, OmpF and OmpC, which we study intensively [ 7 , 8 , 9 , 10 , 11 ]. These porins consist of 16-stranded β-barrel trimers, each of which forms a central channel [ 12 ].…”
Section: Introductionmentioning
confidence: 99%
“…The balance of major OMPs, known as OmpF and OmpC, is achieved through its complex regulation often reciprocal in response to many environmental factors, such as temperature, osmotic pressure, and pH (Liu and Ferenci 2001). Alteration in the porin balance has been observed for a number of clinical isolates making contribution to the adaptive bacterial response to the treatment (Pagès et al 2008;Bystritskaya et al 2014). In addition to major general porins, there are minor ones expressed at very low levels (Prilipov et al 1998).…”
Section: Original Researchmentioning
confidence: 99%
“…; Bystritskaya et al. ). In addition to major general porins, there are minor ones expressed at very low levels (Prilipov et al.…”
Section: Introductionmentioning
confidence: 98%
“…[5][6][7][8] The reciprocal expression of two general porins, OmpC and OmpF, is one of the common ways that bacterial cells adapt to changing environmental conditions. [9][10][11] For example, OmpF (which has a large pore size) is mainly expressed at a temperature of 4 °C-25 °C and when there is a lack of nutrients in the medium, while OmpC (which has a smaller pore size) is primarily expressed at the temperature of a warm-blooded organism (37 °C) and during nutrient excess, 12 OmpC plays a more significant role in bile resistance than OmpF. 13 Therefore, these proteins may contribute to the adaptation of bacterial pathogens to different hosts and different environmental conditions.…”
Section: Introductionmentioning
confidence: 99%