2006
DOI: 10.1038/sj.emboj.7601114
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Regulation of αA-crystallin via Pax6, c-Maf, CREB and a broad domain of lens-specific chromatin

Abstract: Pax6 and c-Maf regulate multiple stages of mammalian lens development. Here, we identified novel distal control regions (DCRs) of the aA-crystallin gene, a marker of lens fiber cell differentiation induced by FGF-signaling. DCR1 stimulated reporter gene expression in primary lens explants treated with FGF2 linking FGF-signaling with aA-crystallin synthesis. A DCR1/aA-crystallin promoter (including DCR2) coupled with EGFP virtually recapitulated the expression pattern of aA-crystallin in lens epithelium and fib… Show more

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Cited by 94 publications
(146 citation statements)
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“…Meis2 has been reported to regulate Pax6 activity in the lens in a direct and positive way (16). Pax6, in turn, controls the expression of genes involved in lens differentiation, including Sox2, Prox1, and α-ACrystallin (17,18). With the exception of Meis2, none of these genes are predicted to contain miR-204 target sites.…”
Section: Mir-204 Controls Lens Differentiation By Targeting Meis2 Andmentioning
confidence: 99%
“…Meis2 has been reported to regulate Pax6 activity in the lens in a direct and positive way (16). Pax6, in turn, controls the expression of genes involved in lens differentiation, including Sox2, Prox1, and α-ACrystallin (17,18). With the exception of Meis2, none of these genes are predicted to contain miR-204 target sites.…”
Section: Mir-204 Controls Lens Differentiation By Targeting Meis2 Andmentioning
confidence: 99%
“…The αA-crystallin (Cryaa) promoter has been shown to bind and to be activated by Pax6 in vitro (Cvekl et al, 1995;Yang and Cvekl, 2005;Yang et al, 2006). Accordingly, Pax6 was found to be required in vivo for the onset of Cryaa expression during early stages of lens development (Ashery-Padan et al, 2000;Cvekl et al, 1995).…”
Section: Pax6 Is Not Required For the Regulation Of Crystallin Genes mentioning
confidence: 99%
“…4). The requirement for Pax6 for the onset of LFC differentiation can be explained by the recently proposed chromatin remodeling model (Yang et al, 2006), according to which TFs operate in a temporal order on enhancer sequences of the Cryaa gene, each TF enabling chromatin remodeling and activity of further TFs. In Pax6 lox/lox ;Mlr10 mutants, Pax6 might enable basal expression of Cryaa and Cryab by 'opening' chromatin to transcription prior to mutation onset in LE cells.…”
Section: Research Articlementioning
confidence: 99%
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“…How this integration of several signaling pathways works at the molecular level is not yet fully understood. A possible mechanism for the establishment of lens-specific expression was recently demonstrated by Yang and co-workers, through their studies of the ␣A-crystallin gene (Cryaa) (Yang et al, 2006). Pax6 initially binds the cis-regulatory elements of Cryaa.…”
Section: Upstream Of Foxe3mentioning
confidence: 99%