Regulation, sensory domains and roles of two <i>Desulfovibrio desulfuricans</i> ATCC27774 Crp family transcription factors, HcpR1 and HcpR2, in response to nitrosative stress

Cadby, Ian T.; Ibrahim, S. A.; Faulkner, Matthew; Lee, David J.; Browning, Douglas F.; Busby, Stephen J. W.; Lovering, Andrew L.; Stapleton, Melanie R.; Green, Jeffrey; Cole, Jeffrey A.

doi:10.1111/mmi.13540

Cited by 13 publications

(19 citation statements)

References 65 publications

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“…We previously showed that HcpR1 binds to a DNA fragment containing the sequence immediately upstream of the D. desulfuricans 27774 hcpR1 gene, suggesting that HcpR1 regulates its own synthesis 30 . We also reported the presence of three similar sequences in the napC regulatory region of this strain 14 .…”

Section: Resultsmentioning

confidence: 99%

“…As the addition of NO also caused a lag in growth, bacteria for RNA analysis were harvested after growth had resumed, which was 4 h after the addition of NO when most of the NO had been reduced. Quantitative RT-PCR data had previously shown that similar transcription responses were detected for more than 20 h after NO addition 30 , so the 4 h exposure allowed the response to NO to be determined. Note, however, that nitrite was generated in cultures supplemented with nitrate, some of which was converted to NO, though the majority was reduced to ammonia.…”

Section: Resultsmentioning

confidence: 99%

“…Two of the 5 transcription factors of the Crp-FNR family encoded in the D. desulfuricans 27774 genome, HcpR1 and HcpR2, are both predicted to regulate the response to nitrosative stress. Correlations were noted between the ability of sulfate reducing bacteria to reduce nitrate, their ability to survive in the human body, and the presence of genes for both HcpR1 and HcpR2 30 . A fascinating result from the previous study was that although NO induced expression of the hcp and hcpR1 genes, HcpR2 was shown to be the transcription factor that regulates Hcp synthesis, but hcpR2 transcription is not induced by NO.…”

Section: Introductionmentioning

confidence: 99%

“…HcpR1 binds to a perfect inverted repeat sequence, 5′-TGTGA-N6-TCACA, which is identical to the consensus binding site for the c-AMP receptor protein, CRP, in Escherichia coli 14 , 19 , 30 . Binding of HcpR1 to this site regulates hcpR1 transcription 19 , 30 . There are also inverted repeat sequences predicted to be the binding sites for HcpR1 immediately upstream of the napC promoter, suggesting that HcpR1 might regulate nitrate reduction as well as its own synthesis.…”

Section: Introductionmentioning

confidence: 99%

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Coordinated response of the Desulfovibrio desulfuricans 27774 transcriptome to nitrate, nitrite and nitric oxide

Cadby

Faulkner

Chèneby

et al. 2017

Sci Rep

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The sulfate reducing bacterium Desulfovibrio desulfuricans inhabits both the human gut and external environments. It can reduce nitrate and nitrite as alternative electron acceptors to sulfate to support growth. Like other sulphate reducing bacteria, it can also protect itself against nitrosative stress caused by NO generated when nitrite accumulates. By combining in vitro experiments with bioinformatic and RNA-seq data, metabolic responses to nitrate or NO and how nitrate and nitrite reduction are coordinated with the response to nitrosative stress were revealed. Although nitrate and nitrite reduction are tightly regulated in response to substrate availability, the global responses to nitrate or NO were largely regulated independently. Multiple NADH dehydrogenases, transcription factors of unknown function and genes for iron uptake were differentially expressed in response to electron acceptor availability or nitrosative stress. Amongst many fascinating problems for future research, the data revealed a YtfE orthologue, Ddes_1165, that is implicated in the repair of nitrosative damage. The combined data suggest that three transcription factors coordinate this regulation in which NrfS-NrfR coordinates nitrate and nitrite reduction to minimize toxicity due to nitrite accumulation, HcpR1 serves a global role in regulating the response to nitrate, and HcpR2 regulates the response to nitrosative stress.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Coordinated response of the Desulfovibrio desulfuricans 27774 transcriptome to nitrate, nitrite and nitric oxide

Cadby

Faulkner

Chèneby

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…In sulfate reducing bacteria, hcp expression is regulated by HcpR2, an Fe-S cluster containing member of the CRP/FNR regulatory family that has been shown to be sensitive to NO and O2 [224].…”

Section: <Figure 15 Here>mentioning

confidence: 99%