Regulations of granule-bound starch synthase I gene expression in rice leaves by temperature and drought stress

Wang, Shu‐Jen; Liu, L. F.; Chen, C. K.; Chen, L. W.

doi:10.1007/s10535-006-0085-2

Cited by 28 publications

(21 citation statements)

References 29 publications

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“…The expression of GBSSI has been found to be regulated by environmental factors in some plants (Hirano and Sano 1998;Wang et al 2001Wang et al , 2006. Expression of GBSSII may be controlled by the supply of nitrogen or sugar .…”

Section: Discussionmentioning

confidence: 99%

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Molecular regulation of starch accumulation in rice seedling leaves in response to salt stress

Chen

Wang

2007

Acta Physiol Plant

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In this report we show that the starch content decreased in NaCl-stressed rice (Oryza sativa L.) seedling leaves during the daytime. Because photosynthetic efficiency and starch degradation enzyme activity were not significantly affected by the high salt, it is likely that this effect results from repression of starch biosynthesis. To determine the regulatory mechanism, the activities of enzymes such as ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) involved in starch synthetic pathway were examined. Data suggest that NaCl-induced repression of GBSS activity was the most significant factor reducing starch accumulation. Based on real-time RT-PCR analysis, the effect of salinity on GBSS expression was primarily controlled on the transcriptional level. Furthermore, the salt-induced decrease of both GBSSI and GBSSII gene expressions could be mostly contributed by ion-specific effect and not by osmotic stress. Although the mRNA accumulation of GBSSI and GBSSII can be down-regulated by exogenous ABA, the negative influence of salt stress on GBSSI and II gene expression could be chiefly mediated via an ABAindependent pathway.

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Section: Discussionmentioning

confidence: 99%

“…Leaf samples (100 mg) were homogenized in 1 ml Trizol reagent (Invitrogen, San Diego, CA, USA) for total RNA extraction according to the protocol described in a previous report (Wang et al 2006).…”

Section: Rna Extraction From Rice Leavesmentioning

confidence: 99%

Molecular regulation of starch accumulation in rice seedling leaves in response to salt stress

Chen

Wang

2007

Acta Physiol Plant

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“…Over expressing this enzyme for greater temperature tolerance may possibly result in starch having more of amylose than amylopectin which is not desirable from the point of view of starch quality (Shewmaker andStalker 1992, Morell et al 2001). In rice leaves however, GBSS I gene expression was found to be higher at low temperature and decreased at temperatures higher than 30 °C (Wang et al 2006). …”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Starch synthase activity and heat shock protein in relation to thermal tolerance of developing wheat grains

Sumesh¹,

Sharma–Natu²,

Ghildiyal³

2008

Biologia plant.

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Wheat (Triticum aestivum L.) cvs. HD 2285 (relatively tolerant) and WH 542 (susceptible) were exposed to ambient and elevated temperature (3 -4 °C higher) in open top chambers during post anthesis period. The grain yield components were determined at the time of maturity. In order to elucidate the basis of differential tolerance of these cultivars, the excised developing grains (20 d after anthesis) of ambient grown plants were incubated at 15, 25, 35 and 45 °C for 2 h and then analysed for the activities of soluble starch synthase (SSS), granule bound starch synthase (GBSS), kinetic parameters of SSS and content of heat shock protein (HSP 100). The elevated temperature during grain development significantly decreased grain growth in WH 542 whereas no such decrease was observed in HD 2285. High temperature tolerance of HD 2285 was found to be associated with higher catalytic efficiency (V max /K m ) of SSS at elevated temperature and higher content of HSP 100.

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“…Micro-Tom), AGP gene expression (isoforms, AgpL1, AgpL2 and AgpS1) and AGPase activity were up-regulated when plants were subjected to 160 mM NaCl, causing a decrease in starch content in the fruit tissues. Also, the expression of GBSS gene in rice was up-regulated when plants were subjected to low temperature (15/10°C; day/night temperature), whereas it was down-regulated in plants exposed to high temperatures (>30/25°C; day/night temperature) and drought stress (Wang et al 2006). Starch degradation in salt-stressed plants may play a role as a major source of carbon, indicated by enriched soluble sugar (3 times that of the control), including glucose, fructose and sucrose (Yin et al 2010).…”

Section: Expression Levels Of Starch Involving Genes Under Salt Stressmentioning

confidence: 99%

Transcriptional regulations of the genes of starch metabolism and physiological changes in response to salt stress rice (Oryza sativa L.) seedlings

Theerawitaya

Boriboonkaset

Cha–um

et al. 2012

Physiol Mol Biol Plants

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The aim of this investigation was to compare the transcriptional expression of starch metabolism, involving genes and physiological characters, in seedlings of two contrasting salt-tolerant rice genotypes, in response to saltstress. The soluble sugar content in rice seedlings of both salt-tolerant and salt-sensitive genotypes was enriched, relating to starch degradation, in plants subjected to 200 mM NaCl. In the salt-tolerant cultivar Pokkali, a major source of carbon may be that derived from the photosynthetic system and starch degradation. In starch degradation, only Pho and PWD genes in Pokkali were upregulated in plants subjected to salt stress. In contrast, the photosynthetic abilities of IR29 salt-susceptible cultivar dropped significantly, relating to growth reduction. The major source of carbohydrate in salt-stressed seedlings of the IR29 cultivar may be gained from starch metabolism, regulated by ADP-glucose pyrophosphorylase (AGP), starch synthase (SS), starch branching enzyme (SBE), starch debranching enzyme (ISA), glucan-water dikinase (GWD), dispropotionating enzyme (DPE), phospho glucan-water dikinase (PWD) and starch phosphorylase (Pho). Also, the major route of soluble sugar in salt-stressed Pokkali seedlings was derived from photosynthesis and starch metabolism. This was identified as novel information in the present study.

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Regulations of granule-bound starch synthase I gene expression in rice leaves by temperature and drought stress

Cited by 28 publications

References 29 publications

Molecular regulation of starch accumulation in rice seedling leaves in response to salt stress

Molecular regulation of starch accumulation in rice seedling leaves in response to salt stress

Starch synthase activity and heat shock protein in relation to thermal tolerance of developing wheat grains

Transcriptional regulations of the genes of starch metabolism and physiological changes in response to salt stress rice (Oryza sativa L.) seedlings

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