Regulators of Oxidative Stress Response Genes in<i>Escherichia Coli</i>and Their Conservation in Bacteria

Schellhorn, Herb E.; Mohiuddin, Mahi M.; Hammond, Sarah M.; Botts, Steven R.

doi:10.1002/9781119004813.ch60

Cited by 3 publications

(2 citation statements)

References 52 publications

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“…The inactivity of glutaredoxin 2 in E. coli cells were more sensitive to hydrogen peroxide and other oxidants, and the interconnection between catalases and thioredoxin/glutaredoxin pathways in the antioxidant response was observed (Vlamis-Gardikas et al, 2002). Regulators including OxyR, SoxRS, and RpoS in E. coli were associated with the tolerance to oxidative stress (Chiang and Schellhorn, 2012). The redox proteins such as Grx A (Grx1) required for maintaining redox status in bacteria also protect bacteria from oxidative stress (Caldas et al, 2006; Meyer et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus

Zhang

Tong

et al. 2019

Front. Microbiol.

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Cronobacter species are linked with life-treating diseases in neonates and show strong tolerances to environmental stress. However, the information about factors involved in oxidative tolerance in Cronobacter remains elusive. Here, factors involved in oxidative tolerance in C. malonaticus were identified using a transposon mutagenesis. Eight mutants were successfully screened based on a comparison of the growth of strains from mutant library ( n = 215) and wild type (WT) strain under 1.0 mM H 2 O 2 . Mutating sites including thioredoxin 2, glutaredoxin 3, pantothenate kinase, serine/threonine protein kinase, pyruvate kinase, phospholipase A, ferrous iron transport protein A, and alanine racemase 2 were successfully identified by arbitrary PCR and sequencing alignment. Furthermore, the comparison about quantity and structure of biofilms formation among eight mutants and WT was determined using crystal violet staining (CVS), scanning electron microscopy (SEM), and confocal laser scanning microscopy (CLSM). Results showed that the biofilms of eight mutants significantly decreased within 48 h compared to that of WT, suggesting that mutating genes play important roles in biofilm formation under oxidative stress. The findings provide valuable information for deeply understanding molecular mechanism about oxidative tolerance of C. malonaticus .

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Section: Resultsmentioning

confidence: 99%

Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus

Zhang

Tong

et al. 2019

Front. Microbiol.

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“…Catalase gene expression is also governed by oxyR. SoxRS is hypothesized to combat superoxide anion, including the upregulation of superoxide dismutase (Schellhorn et al, 2016). Both oxyR and soxRS are present within the Vibrionaceae (Vattanaviboon et al, 2003;Chiang and Schellhorn, 2012).…”

Section: Oxidative Stressmentioning

confidence: 99%

Environmental Stress Selects for Innovations That Drive Vibrio Symbiont Diversity

Soto

Nishiguchi²

2021

Front. Ecol. Evol.

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Symbiotic bacteria in the Vibrionaceae are a dynamic group of γ-Proteobacteria that are commonly found throughout the world. Although they primarily are free-living in the environment, they can be commonly found associated with various Eukarya, either as beneficial or pathogenic symbionts. Interestingly, this dual lifestyle (free-living or in symbiosis) enables the bacteria to have enormous ecological breadth, where they can accommodate a variety of stresses in both stages. Here, we discuss some of the most common stressors that Vibrio bacteria encounter when in their free-living state or associated with an animal host, and how some of the mechanisms that are used to cope with these stressors can be used as an evolutionary advantage that increases their diversity both in the environment and within their specific hosts.

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Inactivation of spoilage organisms on baby spinach leaves using high voltage atmospheric cold plasma (HVACP) and assessment of quality

Sudarsan

Keener

2022

Innovative Food Science & Emerging Technologies

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Regulators of Oxidative Stress Response Genes inEscherichia Coliand Their Conservation in Bacteria

Cited by 3 publications

References 52 publications

Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus

Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus

Environmental Stress Selects for Innovations That Drive Vibrio Symbiont Diversity

Inactivation of spoilage organisms on baby spinach leaves using high voltage atmospheric cold plasma (HVACP) and assessment of quality

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