Regulatory aspects of bakers' yeast metabolism in aerobic fed‐batch cultures

Woehrer, W.; Roehr, M.

doi:10.1002/bit.260230308

Cited by 68 publications

(24 citation statements)

References 11 publications

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“…4, the amount of glucose used for cell maintenance and product formation during exponential growth was ignored (Aiba et al 1976;Woehrer and Roehr 1981). Therefore, if we know the values for cell concentration and working volume at a given time, we can calculate the amount of glucose required for 1 h by using Eq.…”

Section: Control Scheme To Maintain Constant Specific Growth Ratementioning

confidence: 99%

Growth rate control in fed-batch cultures of recombinant Saccharomyces cerevisiae producing hepatitis B surface antigen (HBsAg)

Gu¹,

Park²,

Kim³

1991

Appl Microbiol Biotechnol

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A recombinant Saccharomyces cerevisiae producing hepatitis B surface antigen (HBsAg) exhibited growth-associated product formation. By controlling the medium feed rate, based on the calculated amount of medium required for 1 h, a constant specific growth rate was obtained in the range of 0.12-0.18 h-1. In order to prolong the exponential growth phase, the medium feed rate was increased exponentially. A fed-batch cultivation method based on the production kinetics of batch culture enhanced HBsAg production ten times more than in batch culture. The reason for the increase can be explained by the fact that the production of HBsAg is expressed as an exponential function of time when the specific growth rate is controlled to a constant value in growth-associated product formation kinetics. In the scale-up of this culture to 91, the specific growth rate could also be maintained constant and the HBsAg production trend was similar to that in a 1-1 culture. However, ethanol accumulation occurred at a late stage in fed-bach culture. Ethanol produced was not reutilized and inhibited further cell growth.

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Section: Control Scheme To Maintain Constant Specific Growth Ratementioning

confidence: 99%

Growth rate control in fed-batch cultures of recombinant Saccharomyces cerevisiae producing hepatitis B surface antigen (HBsAg)

Gu¹,

Park²,

Kim³

1991

Appl Microbiol Biotechnol

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“…Although S. cerevisiae depend on oxidative phosphorylation in glucose-poor environments with only non-fermentable carbon sources, they immediately arrest respiration and accelerate glycolysis if glucose becomes available (Woehrer & Roehr 1981), a phenomenon known as the Crabtree effect (Crabtree 1929). Another well studied example is blood stage malaria parasites: they predominantly use glucose fermentation instead of oxidative phosphorylation for ATP production (Sherman 1998).…”

Section: Introductionmentioning

confidence: 99%

Aerobic Glycolysis: Meeting the Metabolic Requirements of Cell Proliferation

Lunt

Heiden

2011

Annu. Rev. Cell Dev. Biol.

2,536

2,296

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Warburg's observation that cancer cells exhibit a high rate of glycolysis even in the presence of oxygen (aerobic glycolysis) sparked debate over the role of glycolysis in normal and cancer cells. Although it has been established that defects in mitochondrial respiration are not the cause of cancer or aerobic glycolysis, the advantages of enhanced glycolysis in cancer remain controversial. Many cells ranging from microbes to lymphocytes use aerobic glycolysis during rapid proliferation, which suggests it may play a fundamental role in supporting cell growth. Here, we review how glycolysis contributes to the metabolic processes of dividing cells. We provide a detailed accounting of the biosynthetic requirements to construct a new cell and illustrate the importance of glycolysis in providing carbons to generate biomass. We argue that the major function of aerobic glycolysis is to maintain high levels of glycolytic intermediates to support anabolic reactions in cells, thus providing an explanation for why increased glucose metabolism is selected for in proliferating cells throughout nature.

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“…Dosing the next molasses portions after the yeast used up the previous ones resulted in the high conversion of molasses compounds into biomass. In exit-gas ethanol was not detected or its concentration was below 0.005 % what indicated that the overfeeding did not occur and pure oxygen respiration took place (Woehrer and Roehr, 1981). The feed rate was not forced but was precisely fitted with Fig.3.…”

Section: Resultsmentioning

confidence: 99%

A real-time feed rate control system for pilot plant baker's yeast production

Miśkiewicz

Wilczyński²

1996

Biotechnol Lett

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An on-line feed rate control system for baker's yeast production using the molasses uptake rate as a feeding index was developed. The optimal feed rate was obtained by maximising the feeding index. The experiments were performed to test this control system in fermenter of 30 m3 total capacity. In baker's yeast process 2760 kg M,, was consumed and 2852 kg compressed yeast (D,) was produced. Cell yield, final molasses dilution and final yeast concentration were 1 .O kg D&g MsO, 1:6.5 and 52 g D,,/l, respectively. These results found that the developed feed rate control system is to be successful.

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Regulatory aspects of bakers' yeast metabolism in aerobic fed‐batch cultures

Cited by 68 publications

References 11 publications

Growth rate control in fed-batch cultures of recombinant Saccharomyces cerevisiae producing hepatitis B surface antigen (HBsAg)

Growth rate control in fed-batch cultures of recombinant Saccharomyces cerevisiae producing hepatitis B surface antigen (HBsAg)

Aerobic Glycolysis: Meeting the Metabolic Requirements of Cell Proliferation

A real-time feed rate control system for pilot plant baker's yeast production

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