Regulatory associations between the metabolism of sulfur-containing amino acids and xanthan biosynthesis in<i>Xanthomonas campestris</i>pv. campestris B100

Schulte, Fabian; Leβmeier, Lennart; Voss, Julia; Ortseifen, Vera; Vorhölter, Frank-Jörg; Niehaus, Karsten

doi:10.1093/femsle/fnz005

Cited by 4 publications

(2 citation statements)

References 65 publications

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“…It is not known how DSF regulates cyclic glucan synthesis or how DF signalling influences xanthan. There are additional regulatory effectors of gum expression: the GntR family regulator HpaR1 is an activator of gum gene expression that interacts directly with the gumB promoter (Su et al 2016a) and CysB was recently identified as another transcriptional regulator that is likely to modulate gum gene expression (Schulte et al 2019). These effects of DNA-binding transcriptional regulators are likely complemented by antisense transcripts that have been detected with several start sites, most outstanding for the promoter region upstream of gumB (Alkhateeb et al 2016).…”

Section: Regulation Of Cell Surface Carbohydrate Biosynthesismentioning

confidence: 99%

“… 2016a ) and CysB was recently identified as another transcriptional regulator that is likely to modulate gum gene expression (Schulte et al . 2019 ). These effects of DNA-binding transcriptional regulators are likely complemented by antisense transcripts that have been detected with several start sites, most outstanding for the promoter region upstream of gumB (Alkhateeb et al .…”

Section: Xanthomonas Eps and Lpsmentioning

confidence: 99%

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Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogenXanthomonas

Potnis

Dow

et al. 2019

FEMS Microbiology Reviews

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Xanthomonas is a well-studied genus of bacterial plant pathogens whose members cause a variety of diseases in economically important crops worldwide. Genomic and functional studies of these phytopathogens have provided significant understanding of microbial-host interactions, bacterial virulence and host adaptation mechanisms including microbial ecology and epidemiology. In addition, several strains of Xanthomonas are important as producers of the extracellular polysaccharide, xanthan, used in the food and pharmaceutical industries. This polymer has also been implicated in several phases of the bacterial disease cycle. In this review, we summarise the current knowledge on the infection strategies and regulatory networks controlling virulence and adaptation mechanisms from Xanthomonas species and discuss the novel opportunities that this body of work has provided for disease control and plant health.

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Section: Regulation Of Cell Surface Carbohydrate Biosynthesismentioning

confidence: 99%

Section: Xanthomonas Eps and Lpsmentioning

confidence: 99%

Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogenXanthomonas

Potnis

Dow

et al. 2019

FEMS Microbiology Reviews

Self Cite

199

165

View full text Add to dashboard Cite

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Molecular Basis and Genetic Regulation of EPS

Kamat

2021

Microbial Exopolysaccharides as Novel and Significant Biomaterials

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Examination of the Global Regulon of CsrA in Xanthomonas citri subsp. citri Using Quantitative Proteomics and Other Approaches

Zhang

Andrade

Wang

et al. 2021

MPMI

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The RNA-binding protein CsrA is a global post-transcriptional regulator and controls many physiological processes and virulence traits. Deletion of csrA caused loss of virulence, reduced motility and production of xanthan gum, substantial increase in glycogen accumulation as well as enhanced bacterial aggregation and cell adhesion in Xanthomonas. How CsrA controls these traits is poorly understood. In this study, an iTRAQ-based proteomic analysis was conducted to compare the protein profile of wild-type strain X. citri subsp. citri (Xcc) and isogenic ∆csrA strain. Totally, 2374 proteins were identified, and 284 were considered to be differentially expressed proteins (DEP¬S), among which 151 proteins were up-regulated and 133 were down-regulated in ∆csrA strain with respect to wild-type strain. Enrichment analysis and protein-protein interaction (PPI) network analysis showed that CsrA regulates bacterial secretion systems, flagella, and xanthan gum biosynthesis. Several proteins encoded by the gumB operon were down-regulated whereas proteins associated with flagellum assembly and type IV secretion system (T4SS) were up-regulated in the ∆csrA strain relative to Xcc306. These results were confirmed by GUS assay or western blot. RNA secondary structure prediction and gel shift assay indicated that CsrA binds to the Shine-Dalgarno (SD) sequence of virB5. In addition, the iTRAQ analysis identified 248 DEPs that were not previously identified in transcriptome analyses. Among them, CsrA regulates levels of 8 regulatory proteins (ColR, GacA, GlpR, KdgR, MoxR, PilH, RecX, and YgiX), 7 TonB-dependent receptors, and 2 ferric enterobactin receptors. Taken together, this study greatly expands understanding of the regulatory network of CsrA in Xcc.

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Regulatory associations between the metabolism of sulfur-containing amino acids and xanthan biosynthesis inXanthomonas campestrispv. campestris B100

Cited by 4 publications

References 65 publications

Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogenXanthomonas

Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogenXanthomonas

Molecular Basis and Genetic Regulation of EPS

Examination of the Global Regulon of CsrA in Xanthomonas citri subsp. citri Using Quantitative Proteomics and Other Approaches

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