2019
DOI: 10.1101/865527
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Regulatory asymmetry in the negative single-input module network motif: Role of network size, growth rate and binding affinity

Abstract: The single-input module (SIM) is a regulatory motif capable of coordinating gene expression across functionally related genes. We explore the relationship between regulation of the central autoregulated TF in a negatively regulated SIM and the target genes using a synthetic biology approach paired with stochastic simulations. Surprisingly, we find a fundamental asymmetry in the level of regulation experienced by the TF gene and its targets, even if they have identical regulatory DNA; the TF gene experiences st… Show more

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Cited by 1 publication
(2 citation statements)
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References 79 publications
(67 reference statements)
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“…In this manuscript, we have investigated how gene copy number variation and presence of decoy sites together modulate the dynamical properties of three well-known regulatory motifs. Copy number variation of genes and addition of decoy sites invoke a competition between the promoters and decoys for the pool of regulatory proteins in the system [30,33,34]. Such resource sharing couples the dynamics of genetic circuits with cell physiology in general, and provides a rich context in which these circuits operate in cells [57].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In this manuscript, we have investigated how gene copy number variation and presence of decoy sites together modulate the dynamical properties of three well-known regulatory motifs. Copy number variation of genes and addition of decoy sites invoke a competition between the promoters and decoys for the pool of regulatory proteins in the system [30,33,34]. Such resource sharing couples the dynamics of genetic circuits with cell physiology in general, and provides a rich context in which these circuits operate in cells [57].…”
Section: Discussionmentioning
confidence: 99%
“…A recent study explored the effect of gene copy number fluctuation [30] on the dynamical properties of well-known regultory motifs. On the other hand, several papers in the recent past have considered the effect of decoy binding [5,12,18,[31][32][33][34][35] in understanding the behavior of specific regulatory motifs. A quantitative understanding of how the interplay between CNV and decoy binding dictate the dynamical properties of gene regulatory motifs remains lacking.…”
Section: Introductionmentioning
confidence: 99%