Regulatory effect and mechanism of LncRNA SOX2OT in idiopathic pulmonary fibrosis

Abstract

Materials and Methods Cell dataHuman embryonic lung FBs MRC5, ordered from the American Type Culture Collection (ATCC), were cultured in the supporting culture medium. Trypsin was added for digestion when the cells were 80-90% confluent, followed by supernatant removal via centrifugation and the addition of 10% FBS-supplemented MEM medium for passage at a 1:2 ratio and further culture. Polymerase chain reaction (PCR) testingTotal cell RNA was extracted by Trizol, and reverse transcribed into cDNA after its purity determination by ultraviolet spectrophotometer. Reverse transcription of 20 μg total RNA was then performed at 50℃ for 2 min, 95℃ for 10 min, 95℃ for 15s, and 60℃ for 60s, for 60 cycles. SOX2OT expression was calculated by 2 -ΔΔCt with GAPDH as an internal reference. Primer sequences, are