Regulatory effect of β-catenin on proliferation of hair follicle stem cells involves PI3K/Akt pathway

Zhang, Yi; Yü, Jin; Shi, Chunying; Wang, Yun; Yang, Jin; Yang, Tian

doi:10.2478/v10136-012-0019-6

Cited by 9 publications

(11 citation statements)

References 41 publications

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“…Previous studies have demonstrated that Akt regulates expression of β-catenin and the β-catenin signaling also participates in the regulation of Akt expression (18)(19)(20). Furthermore, it appears β-catenin regulates cell proliferation via activation of the PI3K/Akt pathway (21). In the present study, overexpression of β-catenin in the late passage of bmMSCs also enhanced Akt1 expression.…”

Section: Discussionsupporting

confidence: 71%

Downregulation of β-catenin and Akt signaling is responsible for poor proliferation of the late passage of bone marrow mesenchymal stem cells

Niu

et al. 2014

Molecular Medicine Reports

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It is well established that mesenchymal stem cells (MSCs) will partially lose their proliferative ability with continuous expansion. However, the specific mechanisms underlying this effect remain unclear. In the present study, it was identified that β-catenin was downregulated in the late passage (passage 8) of bone marrow mesenchymal stem cells (bmMSCs). Following β-catenin expression, the expression of phospho-Akt was also significantly decreased in the late passage of bmMSCs. More notably, overexpression of β-catenin in passage 8 of bmMSCs by transfection with pMXs-β-catenin plasmids, significantly increased cell proliferation and Akt expression. These results indicate that the downregulation of β-catenin and Akt signaling may be a critical factor for the proliferation of the late passage of bmMSCs.

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Section: Discussionsupporting

confidence: 71%

Downregulation of β-catenin and Akt signaling is responsible for poor proliferation of the late passage of bone marrow mesenchymal stem cells

Niu

et al. 2014

Molecular Medicine Reports

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“…It is well-known that activating the Wnt/β-catenin signaling pathway by inhibiting GSK-3β induces β-catenin phosphorylation and degradation, promoting DPC proliferation [ 15 , 20 , 23 , 30 ]. Therefore, we examined the protein levels of β-catenin, phosphorylated GSK-3β (p-GSK-3β [SER9]), p-AKT, and cyclin D1, which are all known to mediate cell proliferation, by western blot analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Androgenetic alopecia is characterized by small-sized hair follicles and a shortening anagen phase. A recent study showed that a hair follicle went into a new anagen from the last telogen when the quiescent stem cells were stimulated to promote proliferation in response to signals from the DP [ 10 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…By activating the Wnt/β-catenin pathway in DPCs, elevated hair growth was observed. In a previous study, the inhibition of GSK-3β by valproic acid and lithium chloride (LiCl) mimicked the effect of Wnt signaling and promoted hair regeneration [ 15 , 30 , 31 , 32 ]. Previous reports suggest that CM from Wnt1a overexpressing MSCs activates DPCs and promotes regeneration of the hair follicle [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth

Kwak

Kim

et al. 2020

Cells

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Conventional therapeutic applications of mesenchymal stromal cells (MSCs) focus on cell replacement and differentiation; however, increasing evidence suggests that most of their therapeutic effects are carried out by their various secretions. This study investigated the application of conditioned medium (CM) from human umbilical cord blood-derived MSCs (hUCB-MSCs) to improve hair growth and developed a method to reliably produce this optimized CM. Primed MSC-derived CM (P-CM) with combinations of TGF-β1 and LiCl was optimized by comparing its effects on the cell viability of dermal papilla cells (DPCs). P-CM significantly increased the viability of DPCs compared to CM. The secretion of vascular endothelial growth factor (VEGF) in DPCs was regulated by the macrophage migration inhibitory factor (MIF) in the P-CM secreted by MSCs. These findings suggest that P-CM can improve the efficacy in hair growth via a paracrine mechanism and that MIF in P-CM exerts hair growth-promoting effects via a VEGF-related β-catenin and p-GSK-3β [SER9] signaling pathway. Furthermore, clinical trials have shown that 5% P-CM improved androgenetic alopecia through producing an increased hair density, thickness, and growth rate, suggesting that this topical agent may be a novel and effective treatment option for patients with androgenetic alopecia.

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“…Several signal pathways and molecules were implicated in regulating the differentiation of hair follicle stem cells into hair follicle lineages, such as Wnt/β-catenin [6], Lef1 [7], Lhx2 [8], TCF3 [9,10], Wnt10b [11], and c-myc [12]. Additionally, non-coding RNAs might play roles in regulating the differentiation of hair follicle stem cells into hair follicle lineages, like miR-22 [13], and LncRNA-PlncRNA 1 [6].…”

Section: Introductionmentioning

confidence: 99%

CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

Yin

Zhao

Jiang

et al. 2020

Animals

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Circular RNAs (CircRNAs) are a type of non-coding RNAs, which contain a covalently closed loop structure without 5′ to 3′ free ends. CircRNAs play essential roles in the regeneration of secondary hair follicle (SHF) and cashmere growth in goats. CircRNA-1926 was previously identified in SHF of cashmere goats, but its potential roles are unclear. In this study, we confirmed the expression of circRNA-1926 in SHF bulge of nine cashmere goats with a significantly higher level at anagen than that of telogen. Through the use of both overexpression and siRNA interference, we showed that circRNA-1926 promoted the differentiation of SHF stem cell into hair follicle lineage in cashmere goats which was evaluated via indictor genes Keratin 7 and Keratin 17. Using RNA pull-down, we found that circRNA-1926 bound with miR-148a/b-3p. Additionally, our data indicated that circRNA-1926 promoted the expression of the CDK19 gene. Using dual-luciferase reporter assays, it was revealed that circRNA-1926 positively regulated the CDK19 expression through miR-148a/b-3p. The results from this study demonstrated that circRNA-1926 contributes the differentiation of SHF stem cells into hair follicle lineages in cashmere goats via sponging miR-148a/b-3p to enhance CDK19 expression.

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Regulatory effect of β-catenin on proliferation of hair follicle stem cells involves PI3K/Akt pathway

Cited by 9 publications

References 41 publications

Downregulation of β-catenin and Akt signaling is responsible for poor proliferation of the late passage of bone marrow mesenchymal stem cells

Downregulation of β-catenin and Akt signaling is responsible for poor proliferation of the late passage of bone marrow mesenchymal stem cells

Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth

CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

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