Regulatory effects of hydrogen sulfide on alveolar epithelial cell endoplasmic reticulum stress in rats with acute lung injury

Abstract

BACKGROUND: The present study was undertaken to examine the regulatory effect of hydrogen sulfi de (H 2 S) on endoplasmic reticulum stress in alveolar epithelial cells of rats with acute lung injury (ALI) induced by oleic acid (OA).METHODS: Seventy-two male Sprague Dawley (SD) rats were divided into control group, oleic acid-induced ALI group (OA group), oleic acid-induced ALI with sodium hydrosulfide (NaHS) pretreatment group (OA+NaHS group), and sodium hydrosulfide treatment group (NaHS group). Rats of each group were further subdivided into 3 subgroups. Index of quantitative assessment of histological lung injury (IQA), wet/dry weight ratio (W/D) and H 2 S level of lung tissues were measured. The expressions of endoplasmic reticulum stress markers including glucose-regulated protein 78 (GRP78) and α-subunit of eukaryotic translation initiation factor-2 (elF2α) in lung tissues were measured by immunohistochemical staining and Western blotting. RESULTS:The IQA score and W/D ratio of lung tissues at the three time points significantly increased in rats injected with OA, but significantly decreased in other rats injected with OA and NaHS. The level of H 2 S in lung tissue at the three time points signifi cantly decreased in rats injected with OA, but signifi cantly increased in other rats injected with both OA and NaHS. GRP78 and elF2α decreased in rats injected with OA, but increased in other rats injected with both OA and NaHS, especially at 4-hour and 6-hour time points. CONCLUSION:The results suggested that H 2 S could promote alveolar epithelial cell endoplasmic reticulum stress in rats with ALI.

Hydrogen Sulfide Plays an Important Protective Role through Influencing Endoplasmic Reticulum Stress in Diseases

“…The decrease of ERS and endogenous H 2 S are involved in the pathogenesis of acute lung injury (ALI). Exogenous H 2 S can play protectice role during the early stage of ALI by increasing ERS, which is contrary to the former statement [87]. The reason needs to be studied (Table 3).…”

Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle

“…In mice, absence of CSE expression promotes AHR. 29 Exogenous administration of H 2 S has been demonstrated as protective in ventilator-induced lung injury, 68,69 acute lung injury, [70][71][72][73][74] hypoxia/hyperoxia environments, [74][75][76] and during acute viral illnesses. [77][78][79] Compared to these data, there is currently no information on H 2 S in the developing bronchial airways per se.…”

Protective effects of hydrogen sulfide inhalation on oxidative stress in rats with cotton smoke inhalation-induced lung injury

“…H 2 S is a harmful gas, commonly associated with the smell of rotten eggs, and has recently emerged as the third gaseous signaling molecule in addition to NO and CO (24,25). Animal experiments have confirmed that intravenous infusion of NaHS or H 2 S inhalation has antioxidative, anti-inflammatory and antiapoptotic effects in animal models of various types of lung injury (2,9,10,26). However, the effects of H 2 S inhalation on SII have not yet been investigated.…”