Regulatory effects of PRF and titanium surfaces on cellular adhesion, spread, and cytokine expressions of gingival keratinocytes

Abstract

Dental implant material has an impact on adhesion and spreading of oral mucosal cells on its surface. Platelet-rich fibrin (PRF), a second-generation platelet concentrate, can enhance cell proliferation and adhesion. The aim was to examine the regulatory effects of PRF and titanium surfaces on cellular adhesion, spread, and cytokine expressions of gingival keratinocytes. Human gingival keratinocytes were cultured on titanium grade 4 (Ti4), titanium grade 5 (Ti5), and HA disks at 37 °C in a CO2 incubator for 6 h and 24 h, using either elutes of titanium-PRF (T-PRF) or leukocyte and platelet rich fibrin (L-PRF), or mammalian cell culture medium (SFM-X) as growth media. Cell numbers were determined using a Cell Titer 96 assay. Interleukin (IL)-1β, IL-1Ra, IL-8, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) expression levels were measured using the Luminex® xMAP™ technique, and cell adhesion and spread by scanning electron microscopy. Epithelial cell adhesion and spread was most prominent to Ti5 surfaces. L-PRF stimulated cell adhesion to HA surface. Both T-PRF and L-PRF activated expressions of IL-1 β, IL-8, IL-1Ra, MCP-1, and VEGF, T-PRF being the strongest activator.Titanium surface type has a regulatory role in epithelial cell adhesion and spread, while PRF type determines the cytokine response.

Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

“…PRF caused a moderate expression of various growth factors in mesenchymal and endothelial cells such as TGFβ [38,46,52,56,65,36], PDGF [23,38,40,46,52,56,65,36], and VEGF [23,37,58]. Dental pulp cells treated with PRF increased expression of dentin sialoprotein and dentin matrix protein 1 [29,34,49].…”

Three‐Dimensional Humanized Model of the Periodontal Gingival Pocket to Study Oral Microbiome

“…SEM micrographs indicated the formation of a basement membrane characterized by stromal cells, which promoted the development of a multi‐layered epithelial structure above the stroma. Analysis of the upper sulcular epithelium showed the protrusion of lamellipodia in keratinocyte cells (Figure 3C higher magnification), [ 28 ] while the remodeling of the connective tissue was demonstrated by new synthesis of collagen fibrils reorganized into individual and mesh‐like fibrils. [ 29 ] Histological characterization of the anatomical model revealed the formation of the sulcular portion of the oral mucosa, a multilayered stratified epithelium tightly bounded by dense underlying connective tissue (Figure 3D), indicating an organization similar to the native gum tissue.…”

Three-dimensional humanized gingival tissue model to study oral microbiome

“…SEM micrographs indicated the formation of a basement membrane characterized by stromal cells, which promoted the development of a multi-layered epithelial structure lying above (Figure 2, C-D) . Analysis of the upper sulcular epithelium showed, in fact, the protrusion of lamellipodia in some of keratinocyte cells (Figure 2, C i ) [33] [34] , while the rearrangement of connective tissue with new synthesis of collagen fibrils reorganized into individual and mesh-like fibrils is shown in Figure 2, D . [35] .…”