Regulatory loop between lncRNA FAS-AS1 and DNMT3b controls FAS expression in hydroquinone-treated TK6 cells and benzene-exposed workers

Yuan, Qian; Zhang, Haiqiao; Pan, Zhijie; Ling, Xiaoxuan; Wu, Ming‐Chi; Gui, Zhiming; Chen, Jialong; Jianming, Peng; Liu, Zhidong; Tan, Qiang; Huang, Dongsheng; Xiu, Liangchang; Chen, Wen; Shi, Zhizhen; Liu, Linhua

doi:10.1016/j.envpol.2020.114147

Cited by 20 publications

(9 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to our previous study, long‐term exposure to HQ was shown to transform TK6 cells into malignant cells, which had different behaviors compared with PBS‐treated TK6 cells 37 . Previous studies have demonstrated that miRNAs and lncRNAs, types of ncRNAs, are both involved in HQ‐induced malignant transformation 12,13 . However, there is no evidence showing that circRNAs are involved in this process.…”

Section: Discussionmentioning

confidence: 97%

“…37 Previous studies have demonstrated that miRNAs and lncRNAs, types of ncRNAs, are both involved in HQ-induced malignant transformation. 12,13 However, there is no evidence showing that circRNAs are involved in this process. CircRNAs belong to a new class of ncRNAs that have attracted significant research attention, as they may play important roles in gene expression and signaling pathways that participate in various disease processes.…”

Section: Discussionmentioning

confidence: 99%

“…Hydroquinone (HQ), also known as one of the main metabolites of benzene in vivo, plays an important role in the toxicity effect of benzene. [3][4][5][6][7] HQ has been shown to cause DNA damage, such as double-strand breaks (DSBs), 8 chromosomal aberration, 9 sister chromatid exchange, 10 and genomic instability, 11 in cultured human and animal cells and expression changes in ncRNAs, such as long noncoding RNAs (lncRNAs) 12 and small noncoding RNAs (miRNAs). 13 However, no studies have yet shown a link between circular RNA (circRNAs) and exposure to HQ.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hsa_circ_0001944 regulates apoptosis by regulating the binding of PARP1 and HuR in leukemia and malignant transformed cells induced by hydroquinone

Zhong

Ling

Meng

et al. 2022

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

Hydroquinone (HQ) is one of the major metabolites of benzene and can cause abnormal gene expression. It is a known carcinogen that alters cell cycle disruption and cell proliferation. However, its chemical mechanism remain a mystery. Circular RNAs (cir-cRNAs) are a subtype of noncoding RNAs (ncRNAs) that play a variety of roles in biological processes. Hsa_circ_001944 expression was upregulated in 30 leukemia patients and HQ-induced malignant transformed TK6 cells. Hsa_circ_001944 silencing inhibited the growth of HQ-TK6 cells and halted the cell cycle. The silencing of hsa_circ_0001944 led to increased cell accumulation in G1 versus S phase, increased apoptosis in the sh1944 versus the shNC group, and increased levels of DNA damage (γ-H2AX), leading to cell cycle arrest. In summary, inhibition of hsa_circ_001944 restricted cell growth by inhibiting cell cycle arrest and induced growth of HQ-TK6 cells by modulating PARP1 expression. Hsa_circ_0001944 targeted HuR, which is a kind of RNA-binding protein, to control PARP1 expression via RNAinter, RBPmap, and RBPdb. Fluorescence in situ hybridization combined with immunofluorescent labeling and western blotting experiments showed that hsa_circ_001944 was able to dissociate HuR and PARP1 binding in HQ-TK6 cells, control PARP1 production, and ultimately alter the PARP1/H-Ras pathway.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hsa_circ_0001944 regulates apoptosis by regulating the binding of PARP1 and HuR in leukemia and malignant transformed cells induced by hydroquinone

Zhong

Ling

Meng

et al. 2022

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Control cells were transfected with a lenti-CRISPR/Cas9 construct without a sgRNA sequence. 23 T A B L E 1 PCR primer sequences.…”

Section: Knockdown Of Dnmts By Crispr/cas9mentioning

confidence: 99%

DNMT1 regulates hypermethylation and silences hsa_circ_401351 in hydroquinone‐induced malignant TK6 cells

Han,

Meng,

Ling

et al. 2023

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

BackgroundBenzene and its metabolite hydroquinone (HQ) are widely used in daily life, and long‐term exposure to benzene or HQ can induce acute myeloid leukemia (AML). Circular RNAs (circRNAs) are mostly produced by reverse splicing of gene exon mRNA precursors. The modulation of circRNA expression is connected to leukemia progression; however, the molecular mechanism is still unknown.Materials and methodsIn this study, the cells were divided into four groups: PBS control group (PBS‐TK6), TK6 malignantly transformed cells induced by 10.0 μmol/L HQ (HQ‐TK6), and HQ‐TK6 cells treated with 5 μmol/L 5‐AzaC (DNA methyltransferase inhibitor) for 24 h (HQ + 5‐AzaC). HQ‐TK6 cells were treated with 200 nmol/L TSA (histone deacetylation inhibitor) for 24 h (HQ + TSA). qRT–PCR was used to identify the differential hsa_circ_401351 expression between the four groups. We further determined the hsa_circ_401351 promoter methylation level with methylation‐specific PCR. DNMT1 and DNMT3b were knocked down by CRISPR/Cas9 to elucidate the specific molecular mechanism of hsa_circ_401351 in HQ‐TK6 cells. CCK‐8 and flow cytometry detected cell proliferation and apoptosis, respectively, after hsa_circ_401351 was overexpressed in HQ‐TK6 cells.ResultsCompared with the PBS‐TK6 group, the expression of hsa_circ_401351 was found to be lower in the HQ‐TK6 group. Nevertheless, treatment with 5‐AzaC or TSA increased hsa_circ_401351 expression, with the upregulation being more pronounced in the TSA group. The expression of hsa_circ_401351 in the DNMT1 knockdown group was dramatically increased by 50% compared to that in the control group, and the DNA methylation level of the hsa_circ_401351 promoter region was decreased. When hsa_circ_401351 was overexpressed, HQ‐TK6 cell proliferation was significantly slowed after 48 h compared with the control group. Flow cytometry showed that cells were mainly arrested in G1 phase, and apoptosis was significantly enhanced. Similarly, qRT–PCR and Western blot data showed significant reductions in Caspase‐3 mRNA and protein production, and Bcl‐2 mRNA levels were also elevated.ConclusionsOverall, our research showed that elevated DNMT1 expression in HQ‐TK6 cells increased methylation levels and decreased expression of the hsa_circ_401351 promoter region, limiting its ability to suppress HQ‐TK6 cell growth and enhance apoptosis.

show abstract

“…With the rapid development of urbanization and industrialization, the emission of volatile organic chemicals (VOCs) has continued to increase in the past decades, which causes serious environmental and human health issues . To reduce VOC emissions, a number of techniques have been developed, which can be divided into destructive (catalytic oxidation, biodegradation, thermal oxidation, and plasma catalysis) and recovery treatments (membrane separation, absorption, adsorption, and condensation). − Compared with the common destructive treatment, recovery of VOCs has been confirmed to be a cost-effective technique based on economic and environmental considerations. , Recovery techniques such as adsorption, absorption, condensation, and membrane separation have been widely studied and developed. Adsorption is generally regarded as a relatively mature technique for economically and effectively removing VOCs, whereas it is often accompanied by adsorbent regeneration requirement and high equipment and maintenance costs. − …”

Section: Introductionmentioning

confidence: 99%

Design of High-Humidity-Proof Hierarchical Porous P-ZIF-67(Co)-Polymer Composite Materials by Surface Modification for Highly Efficient Volatile Organic Compound Adsorption

Wang

et al. 2022

Ind. Eng. Chem. Res.

View full text Add to dashboard Cite

The ubiquitous water vapor in industrial gas is a challenge for volatile organic compound (VOC) adsorbents. Herein, we have developed a strategy to improve the hydrophobicity and water stability of ZIF-67(Co) through internal and external modification. Through amine modification of the carrier surface rich in nanoscale roughness, the shape and size of ZIF-67(Co) are changed. ZIF-67(Co) is uniformly anchored on the macroporous surface of polyacrylates (PA) through interface coordination, forming a unique hierarchical porous structure. Compared with the original ZIF-67(Co), the toluene adsorption efficiency of ZIF-67(Co)@PA is increased by 2.75 times at 298 K and at a toluene partial pressure of P/P 0 = 0.005. Furthermore, to inhibit undesired water adsorption due to the rich defect sites, hydrophobically modified ZIF-67(Co)@PA (yielding P-ZIF-67(Co)@PA) has also been prepared, which exhibits a slight decrease in toluene adsorption and maintains the advantages of low water vapor adsorption and high hydrophobicity to improve the water stability of ZIF-67(Co).

show abstract

Regulatory loop between lncRNA FAS-AS1 and DNMT3b controls FAS expression in hydroquinone-treated TK6 cells and benzene-exposed workers

Cited by 20 publications

References 49 publications

Hsa_circ_0001944 regulates apoptosis by regulating the binding of PARP1 and HuR in leukemia and malignant transformed cells induced by hydroquinone

Hsa_circ_0001944 regulates apoptosis by regulating the binding of PARP1 and HuR in leukemia and malignant transformed cells induced by hydroquinone

DNMT1 regulates hypermethylation and silences hsa_circ_401351 in hydroquinone‐induced malignant TK6 cells

Design of High-Humidity-Proof Hierarchical Porous P-ZIF-67(Co)-Polymer Composite Materials by Surface Modification for Highly Efficient Volatile Organic Compound Adsorption

Contact Info

Product

Resources

About