Regulatory Mechanism of Nicotine Degradation in
            <i>Pseudomonas putida</i>

Hu, Haiyang; Wang, Lijuan; Wang, Weiwei; Wu, Geng; Tao, Fei; Xu, Ping; Deng, Zixin; Tang, Hongzhi

doi:10.1128/mbio.00602-19

Cited by 22 publications

(14 citation statements)

References 26 publications

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“…These species belonged to the genera Pseudomonas , Delftia , Acinetobacter , Enterobacter , Stenotrophomonas , Klebsiella , Chryseobacterium , Exiguobacterium , Lactococcus , Brevibacterium , Kocuria , Methylorubrum , Roseomonas , and Sphingobacterium . Of them, Pseudomonas , Delftia , Stenotrophomonas , Klebsiella , Brevibacterium , and Sphingobacterium were already reported as nicotine degraders ( Ruan et al, 2006 ; Ma, 2012 ; Sabourmoghaddam et al, 2015 ; Gaekwad and Vinchurkar, 2018 ; Hu et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Direct Evidence That Sunbirds’ Gut Microbiota Degrades Floral Nectar’s Toxic Alkaloids

et al. 2021

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Orange-tufted sunbirds (Cinnyris osea) feed on the nectar of the tobacco tree (Nicotiana glauca) which contains toxic pyridine alkaloids characterized by high concentrations of anabasine and much lower concentrations of nicotine. We aimed at determining whether the gut microbiota of sunbirds harbors bacterial species that enable the birds to cope with these toxic alkaloids. An in vivo experiment that included 12 birds showed that inducing dysbiosis in sunbirds’ guts by the addition of sulfamethoxazole and trimethoprim, significantly reduced the birds’ ability to degrade anabasine (n = 3) compared to control birds (n = 3) with undisturbed microbiota. Sunbirds whose gut bacterial communities were altered by the antibacterial agents and who were fed with added nicotine, also showed a lower percentage of nicotine degradation (n = 3) in their excreta compared to the sunbirds with undisturbed microbiota (n = 3), though this difference was not significant. In an in vitro experiment, we studied the ability of Lactococcus lactis, Enterobacter hormaechei, Chryseobacterium gleum, Kocuria palustris, and Methylorubrum populi that were isolated from sunbirds’ excreta, to degrade anabasine and nicotine. By using gas chromatography-mass spectrometry (GC-MS) analysis, we successfully demonstrated, for the first time, the ability of these species to degrade the focal secondary metabolites. Our findings demonstrate the role of gut bacteria in detoxifying toxic secondary metabolites found in the N. glauca nectar. The degradation products may supply the birds with nitrogen which is scarce in nectar-rich diets. These findings support another role of bacteria in mediating the interactions between plants and their pollinators.

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Section: Discussionmentioning

confidence: 99%

Direct Evidence That Sunbirds’ Gut Microbiota Degrades Floral Nectar’s Toxic Alkaloids

et al. 2021

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“…There are various reports of nicotine degradation by different bacterial genera including Delftia, Klebsiella, Stenotrophomonas Pseudomonas and Lactobacillus [43][44][45][46][47] . Here we isolated bacteria capable of growing on nicotine and anabasine as sole carbon and nitrogen sources (Table 2).…”

Section: Discussionmentioning

confidence: 99%

The effect of toxic pyridine-alkaloid secondary metabolites on the sunbird gut microbiome

Mohanraj

Lalzar

Sharaby

et al. 2020

npj Biofilms Microbiomes

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Sunbirds feed on tobacco tree nectar which contains toxic nicotine and anabasine secondary metabolites. Our aim was to understand the effect of nicotine and anabasine on the gut microbiota composition of sunbirds. Sixteen captive sunbirds were randomly assigned to two diets: artificial nectar either with (treatment) or without (control) added nicotine and anabasine. Excreta were collected at 0, 2, 4 and 7 weeks of treatment and samples were processed for bacterial culture and high-throughput amplicon sequencing of the 16S rRNA gene. The gut microbiome diversity of the treated and control birds changed differently along the seven-week experiment. While the diversity decreased in the control group along the first three samplings (0, 2 and 4 weeks), it increased in the treatment group. The microbiota composition analyses demonstrated that a diet with nicotine and anabasine, significantly changed the birds’ gut microbiota composition compared to the control birds. The abundance of nicotine- and anabasine- degrading bacteria in the excreta of the treated birds, was significantly higher after four and seven weeks compared to the control group. Furthermore, analysis of culturable isolates, including Lactococcus, showed that sunbirds’ gut-associated bacteria were capable of degrading nicotine and anabasine, consistent with their hypothesised role as detoxifying and nutritional symbionts.

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“…There are various reports of nicotine degradation by different bacterial genera; for example; Delftia, Klebsiella, Stenotrophomonas Pseudomonas and Lactobacillus [28][29][30]32,35]. Here we have also isolated various bacterial genera (Table 2) that showed the ability to grow on nicotine and anabasine as sole carbon and nitrogen sources and, thus, to degrade these compounds.…”

Section: The Effect Of Secondary Metabolites On the Gut Microbiomementioning

confidence: 82%

The effect of pyridine-alkaloid secondary metabolites on sunbirds gut microbiome

Mohanraj

Lalzar

Sharaby

et al. 2019

Preprint

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Background: Sunbirds (Cinnyris osea) feed on nectar of the tobacco tree (Nicotiana glauca), which contains the secondary metabolites nicotine and anabasine. These toxic pyridine-alkaloids presumably serve to deter nectar thieves and allow only specific pollinators to feed on nectar. The floral nectar contains low level of proteins and hence nectarivores may have problem balancing their nitrogen requirements. Our aim was to understand whether the gut microbiome of nectarivores contains bacteria that are able to degrade plant secondary metabolites and how the presence of secondary metabolites in nectar shapes the gut microbiome of nectarivores.Results: Sunbirds (n=16) that were held in captivity were randomly divided into control and treatment groups. The control group was fed with artificial nectar while the treatment group was fed with artificial nectar with the addition of nicotine and anabasine. Excreta were collected from each of the 16 birds on day 0 and at the end of weeks 2, 4 and 7. Nonmetric multidimensional scaling analysis (nMDS) demonstrated that the secondary metabolites significantly changed the gut microbiota composition and diversity of those sunbirds that were fed nectar with added pyridine alkaloids. Aeromonas, Enterobacteriaceae, Exiguobacterium, Klebsiella, Lactococcus, Leuconostoc, Rothia and Salmonella were found to contribute to the differences in gut microbiota between the control and treated birds (birds feed with Nicotine and Anabasine). Four weeks after starting to feed the birds with added nicotine and anabasine, we found that genera with the ability to degrade these pyridine-type metabolites proliferated from a total relative abundance of only 0.3% to 52.7% of the gut bacterial community.Conclusion: Sunbirds feed on Nicotiana nectar that contains toxic pyridine-alkaloids. Nonetheless, the symbiotic gut microbiota which is changing dynamically towards enabling the degradation of these secondary metabolites, supply the birds with nitrogen that enables the synthesis of essential amino acids. The current study contributes to our understanding of the complex relationships between plants, birds and their microbiomes.

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Regulatory Mechanism of Nicotine Degradation in Pseudomonas putida

Cited by 22 publications

References 26 publications

Direct Evidence That Sunbirds’ Gut Microbiota Degrades Floral Nectar’s Toxic Alkaloids

Direct Evidence That Sunbirds’ Gut Microbiota Degrades Floral Nectar’s Toxic Alkaloids

The effect of toxic pyridine-alkaloid secondary metabolites on the sunbird gut microbiome

The effect of pyridine-alkaloid secondary metabolites on sunbirds gut microbiome

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