Regulatory patterns analysis of transcription factor binding site clustered regions and identification of key genes in endometrial cancer

Exploring distinct properties of endometrial stem cells through advanced single-cell analysis platforms

“…In addition to these conventional epigenomic profiling platforms, assay for transposase-accessible chromatin using sequencing (ATAC-seq) has emerged as a transformative technique for characterizing the gene regulatory landscape and quantifying chromatin accessibility at a single-cell level. Its ability to quantify chromatin accessibility at high resolution, its applicability to heterogeneous samples, and its compatibility with other single-cell techniques make it an invaluable tool for deciphering complex mechanisms governing gene regulation in health and diseases [ 38 ] (Fig. 4 ).…”

“…The ability to study transcription patterns and epigenetic regulation in single cells was limited by technological constraints in the past. However, recent advances in bioanalytical technologies have enabled the study of transcription pattern [ 37 ] and epigenetic regulation [ 38 ] in single cells, which in turn allow researchers to profile gene expression and signaling pathways of individual cells in a heterogeneous population, thereby providing a more detailed understanding of the heterogeneity of the endometrium at a single-cell level. By examining gene expression profiles of individual cells, researchers can identify cell subpopulations and their molecular characteristics, including their differentiation potential, proliferation rates, and interaction with neighboring cells.…”