Regulatory role of adenosine deaminase complexing protein (dipeptidyl peptidase IV = CD26) on the malignancy marker adenosine deaminase: Effect of membrane cholesterol and phase-transition

Parola, Abraham H.; Caiolfa, Valeria R.; Ben-Shooshan, Itzhak; Cohen‐Luria, Rivka

doi:10.1002/1098-2299(200007/08)50:3/4<537::aid-ddr37>3.0.co;2-z

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“…We have previously found that SVMF affected cell proliferation as well as the activity and dynamics of the malignancy marker adenosine deaminase, ADA (Parola et al, 2000;Ben-Shooshan et al, 2002): exposure of CEF to a 100 Hz/0.7 mT SVMF resulted in reduced cell proliferation, decreased activity of ADA, and a remarkable rise of segmental rotational relaxation rate of ADA bound to adenosine deaminase complexing protein (ADCP = DPPIV = CD26) as determined by multifrequency phase fluorometry. The changes in membrane dynamics were characteristics of those observed in malignant viral cell transformation (Parola and Markel, 1994;Porat et al, 1988;Parola et al, 1993;.…”

Section: Introductionmentioning

confidence: 83%

Radical Scavengers Suppress Low Frequency EMF Enhanced Proliferation in Cultured Cells and Stress Effects in Higher Plants

et al. 2005

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In previous publications, we reported that sinusoidal varying magnetic fields (SVMF) modify the activity and dynamics of the malignancy marker adenosine deaminase, and enhance the proliferation of chick embryo fibroblasts (CEF). While the SVMF examined by us (50, 60 & 100 Hz / 0.06-0.7 mT) were all below kT, they may have the potential of altering chemical processes in which excited radicals are involved.We tested this hypothesis in two experimental systems: CEF in culture and Spirodela oligorrhiza (Lemnaceae) (a small aquatic plant, commonly known as Duckweed). CEF were exposed to SVMF of 100 Hz/0.7 mT for 24 h. The addition of the exogenous radical scavengers catalase, superoxide dismutase or vitamin E to the cells during exposure significantly suppressed enhancement of cell proliferation caused by the field (by 79, 67 and 82%, respectively, as evaluated by the MTT colorimetric assay). 15 N NMR analysis of Duckweed plants fed by 15 N-labeled ammonium chloride and exposed to SVMF at 60 and 100 Hz/0.7 mT for 24 h, revealed augmented alanine production. Alanine did not accumulate in the absence of SVMF. The addition of vitamin C, a radical scavenger, reduced alanine production by 82%.Exposure to SVMF resulted in specific metabolic stress effects in Duckweed plants and enhanced proliferation of CEF. In both cases, it is suggested that free radicals are involved.Abbreviations: SVMF = sinusoidal varying magnetic fields; CEF = chick embryo fibroblasts; ADA = adenosine deaminase; SOD = superodxide dismutase.

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Section: Introductionmentioning

confidence: 83%