Regulatory role of cellular and viral microRNAs in insect–virus interactions

Asgari, Sassan

doi:10.1016/j.cois.2014.12.008

Cited by 33 publications

(30 citation statements)

References 62 publications

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“…() discovered the first miRNA, named lin ‐ 4 , in the nematode Caenorhabditis elegans , which provided new insight in miRNA research. Subsequently, miRNAs have been found in animals, plants, and viruses (Asgari, ; He & Hannon, ). At present, most miRNA research mainly focuses on biogenesis, expression patterns, target messenger (m)RNAs and their targeting mechanisms, and physiological functions and applications (Zhang et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex‐related microRNAs

Chen

Huang

Zhu

et al. 2018

Journal of Fish Biology

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This research identified 169 known microRNAs (miRNAs), 380 novel miRNAs, and 30,538 targets in 11 tissues (blood, brain, derma, gill, heart, intestine, kidney, liver, muscle, pronephros, and spleen) from grass carp Ctenopharyngodon idella with high-throughput sequencing (HTS).Transcripts per million (TPM) expression analysis detected 41 brain-enriched miRNAs (accounting for 61.19% of all tissue-enriched miRNAs). Real-time quantitative PCR (RT-qPCR) confirmed that 21 of 24 randomly selected tissue-enriched miRNAs from the TPM analysis were indeed tissue-enriched (P < 0.05), suggesting the HTS and TPM analyses were reliable. Nine of the 41 brain-enriched miRNAs are complementary to members of the double-sex and mab-3 related transcription factor family (dmrt) involved in sex differentiation. RT-qPCR revealed that cid-miR-138 was more highly expressed in testis than in ovary (P < 0.01), while the reverse was true for target gene dmrt4a (P < 0.01). This opposite expression pattern suggested the direct participation of cid-miR-138-dmrt4a in neuroendocrine mechanisms related to brain-pituitary networks during sex development. The discovery of miRNAs from 11 C. idella tissues expands the available fish miRNA database, and enhances our understanding of the role of sex-related miRNAs in tissue differentiation and maintenance of specific tissue functions in fishes. K E Y W O R D Sdeep sequencing, dmrt, grass carp, microRNA, sex differentiation

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Section: Introductionmentioning

confidence: 99%

Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex‐related microRNAs

Chen

Huang

Zhu

et al. 2018

Journal of Fish Biology

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“…Virus-encoded miRNAs may function just as cellular miRNAs and inhibit the translation of cellular mRNAs through direct interaction with its target mRNA, mediated by partial complementarity ( Cullen, 2009 ; Kincaid and Sullivan, 2012 ). In addition, virus-encoded miRNAs can regulate virus encoded genes, especially genes that are involved in regulation of virus replication, and as such may control the latent and lytic infection stages by fully or partially aligning antisense to a target viral mRNA ( Kincaid and Sullivan, 2012 ; He et al, 2014 ; Asgari, 2015 ). The molecular mechanisms of interactions between tsetse flies and GpSGHV are poorly understood, making it difficult to define the factors that determine the switch from asymptomatic to symptomatic infection.…”

Section: Discussionmentioning

confidence: 99%

Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus)

et al. 2018

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The Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) infects tsetse flies predominantly asymptomatically and occasionally symptomatically. Symptomatic infections are characterized by overt salivary gland hypertrophy (SGH) in mass reared tsetse flies, which causes reproductive dysfunctions and colony collapse, thus hindering tsetse control via sterile insect technique (SIT). Asymptomatic infections have no apparent cost to the fly’s fitness. Here, small RNAs were sequenced and profiles in asymptomatically and symptomatically infected G. pallidipes flies determined. Thirty-eight host-encoded microRNAs (miRNAs) were present in both the asymptomatic and symptomatic fly profiles, while nine host miRNAs were expressed specifically in asymptomatic flies versus 10 in symptomatic flies. Of the shared 38 miRNAs, 15 were differentially expressed when comparing asymptomatic with symptomatic flies. The most up-regulated host miRNAs in symptomatic flies was predicted to target immune-related mRNAs of the host. Six GpSGHV-encoded miRNAs were identified, of which five of them were only in symptomatic flies. These virus-encoded miRNAs may not only target host immune genes but may also participate in viral immune evasion. This evidence of differential host miRNA profile in Glossina in symptomatic flies advances our understanding of the GpSGHV-Glossina interactions and provides potential new avenues, for instance by utilization of particular miRNA inhibitors or mimics to better manage GpSGHV infections in tsetse mass-rearing facilities, a prerequisite for successful SIT implementation.

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“…Changes in cellular miRNAs are observed during infections with CPV (Wu et al, 2013) while the production of viral miRNAs by RNA viruses is controversial (Umbach and Cullen, 2009; Asgari, 2015). …”

Section: Types Of Virus Infections In Insects and The Interaction Witmentioning

confidence: 99%

Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges

et al. 2017

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RNAi is applied as a new and safe method for pest control in agriculture but efficiency and specificity of delivery of dsRNA trigger remains a critical issue. Various agents have been proposed to augment dsRNA delivery, such as engineered micro-organisms and synthetic nanoparticles, but the use of viruses has received relatively little attention. Here we present a critical view of the potential of the use of recombinant viruses for efficient and specific delivery of dsRNA. First of all, it requires the availability of plasmid-based reverse genetics systems for virus production, of which an overview is presented. For RNA viruses, their application seems to be straightforward since dsRNA is produced as an intermediate molecule during viral replication, but DNA viruses also have potential through the production of RNA hairpins after transcription. However, application of recombinant virus for dsRNA delivery may not be straightforward in many cases, since viruses can encode RNAi suppressors, and virus-induced silencing effects can be determined by the properties of the encoded RNAi suppressor. An alternative is virus-like particles that retain the efficiency and specificity determinants of natural virions but have encapsidated non-replicating RNA. Finally, the use of viruses raises important safety issues which need to be addressed before application can proceed.

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Regulatory role of cellular and viral microRNAs in insect–virus interactions

Cited by 33 publications

References 62 publications

Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex‐related microRNAs

Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex‐related microRNAs

Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus)

Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges

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