Regulatory role of monoamine neurotransmitters in Saccharomyces cerevisiae cells

Malikina, K.; Shishov, V. A.; Chuvelev, D. I.; Кудрин, В. С.; Oleskin, Alexander V.

doi:10.1134/s0003683810060104

Cited by 24 publications

(28 citation statements)

References 4 publications

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“…Previously it was shown that QN and tryptophan compete for uptake in yeast 5 , and elsewhere that the tryptophan derivative serotonin can stimulate yeast growth 21 . We exploited these insights in some preliminary tests for interference of serotonin action by QN, using the yeasts C. albicans and S. cerevisiae .…”

Section: Resultsmentioning

confidence: 99%

The antimalarial drug quinine interferes with serotonin biosynthesis and action

Islahudin

Tindall

Mellor

et al. 2014

Sci Rep

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The major antimalarial drug quinine perturbs uptake of the essential amino acid tryptophan, and patients with low plasma tryptophan are predisposed to adverse quinine reactions; symptoms of which are similar to indications of tryptophan depletion. As tryptophan is a precursor of the neurotransmitter serotonin (5-HT), here we test the hypothesis that quinine disrupts serotonin function. Quinine inhibited serotonin-induced proliferation of yeast as well as human (SHSY5Y) cells. One possible cause of this effect is through inhibition of 5-HT receptor activation by quinine, as we observed here. Furthermore, cells exhibited marked decreases in serotonin production during incubation with quinine. By assaying activity and kinetics of the rate-limiting enzyme for serotonin biosynthesis, tryptophan hydroxylase (TPH2), we showed that quinine competitively inhibits TPH2 in the presence of the substrate tryptophan. The study shows that quinine disrupts both serotonin biosynthesis and function, giving important new insight to the action of quinine on mammalian cells.

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Section: Resultsmentioning

confidence: 99%

The antimalarial drug quinine interferes with serotonin biosynthesis and action

Islahudin

Tindall

Mellor

et al. 2014

Sci Rep

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“…According to recent literature data, biogenic amines are synthesized and accumulated in microbial cells, including those of E. coli, even if the cultivation medium does not contain them. These concentrations are sufficiently high to enable their binding to receptors in animal /human cells [3,19]. Possibly, the effects that were observed in our test system resulted from the combined action of both exogenous and endogenous (biosensor cell-synthesized) neurotransmitters.…”

Section: Results and Discusionmentioning

confidence: 92%

“…We demonstrated in our recent studies that dopamine stimulated cell proliferation in the eukaryote Saccharomyces cerevisiae, while norepinephrine did not significantly influence this process. The dopamine agonist apomorphine that binds to receptors D 1 and D 2 also was found to stimulate cell proliferation [18,19].…”

Section: Results and Discusionmentioning

confidence: 99%

Testing Neurotransmitters for Toxicity with a Luminescent Biosensor: Implications for Microbial Endocrinology

Oleskin

Сорокина

Zarubina

et al. 2017

J. Pharm. Nutr. Sci.

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Background: The human organism is a complex superorganism including numerous eukaryotic, eubacterial, and archaean cells. The qualitative and quantitative assessment of the microbiota toxicity of chemical agents, i.e., their inhibitory effects on the microbial inhabitants of the human organism in health and disease, seems to hold much value in this context. In this work, a bacterial luminescence-based express test system for microbiota toxicity is applied to neurotransmitters such as serotonin, dopamine, norepinephrine, and histamine. Methods:The biosensor was based on a GM Escherichia coli K12 strain (TGI) that contained the lux operon of the luminescent soil bacterium Photorhabdus luminescencens ZMI. The biosensor was exposed to the action of the tested neurotransmitters for 5 to 60 minutes The intensity of bacterial luminescence (counts . sec -1 ) was monitored in the control and the experimental samples with a Biotoks 6 ms luminometer (Russia); the toxicity index (T) of the neurotransmitters was determined.Results: A marked toxic effect on bioluminescence was produced by serotonin, histamine, and dopamine at concentrations exceeding 80 µg/ml, 100 µg/ml, and 1 mg/ml, respectively. At lower concentration, these neurotransmitters were "negatively toxic", i.e. stimulatory in terms of the effect on bacterial luminescence. In contrast, norepinephrine inhibited luminescence at all concentrations tested. Conclusions:The bacterial luminescence-based testing method is applicable to the assessment of the destructive and stimulatory effects of neurotransmitters; the data obtained are of microbiological and clinical relevance.

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“…The range of neurochemicals and neurohormones isolated from microorganisms is extensive and additionally have been demonstrated to show biological activity in mammalian cells. These include serotonin (Sridharan et al, 2014); catecholamines, such as norepinephrine and dopamine (Asano et al, 2012) (Tsavkelova et al, 2000;Malikina et al, 2010); progesterone (Schar et al, 1986); somatostatin (LeRoith et al, 1985); and even the primary inhibitor neurotransmitter in the brain, γ amino butyric acid (GABA) (Barrett et al, 2012). For a more complete discussion of the ubiquitous presence of neurochemicals throughout the various biological kingdoms see the comprehensive review (Roshchina, 2016).…”

Section: Introductionmentioning

confidence: 99%

Review: Microbial endocrinology: intersection of microbiology and neurobiology matters to swine health from infection to behavior

Lyte

2019

Animal

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From birth to slaughter, pigs are in constant interaction with microorganisms. Exposure of the skin, gastrointestinal and respiratory tracts, and other systems allows microorganisms to affect the developmental trajectory and function of porcine physiology as well as impact behavior. These routes of communication are bi-directional, allowing the swine host to likewise influence microbial survival, function and community composition. Microbial endocrinology is the study of the bi-directional dialogue between host and microbe. Indeed, the landmark discovery of host neuroendocrine systems as hubs of host-microbe communication revealed neurochemicals act as an inter-kingdom evolutionary-based language between microorganism and host. Several such neurochemicals are stress catecholamines, which have been shown to drastically increase host susceptibility to infection and augment virulence of important swine pathogens, including Clostridium perfringens. Catecholamines, the production of which increase in response to stress, reach the epithelium of multiple tissues, including the gastrointestinal tract and lung, where they initiate diverse responses by members of the microbiome as well as transient microorganisms, including pathogens and opportunistic pathogens. Multiple laboratories have confirmed the evolutionary role of microbial endocrinology in infectious disease pathogenesis extending from animals to even plants. More recent investigations have now shown that microbial endocrinology also plays a role in animal behavior through the microbiota-gut-brain axis. As stress and disease are ever-present, intersecting concerns during each stage of swine production, novel strategies utilizing a microbial endocrinology-based approach will likely prove invaluable to the swine industry.

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Regulatory role of monoamine neurotransmitters in Saccharomyces cerevisiae cells

Cited by 24 publications

References 4 publications

The antimalarial drug quinine interferes with serotonin biosynthesis and action

The antimalarial drug quinine interferes with serotonin biosynthesis and action

Testing Neurotransmitters for Toxicity with a Luminescent Biosensor: Implications for Microbial Endocrinology

Review: Microbial endocrinology: intersection of microbiology and neurobiology matters to swine health from infection to behavior

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