Regulatory Role of Sulfated Polysaccharides Fucoidan from Fucus vesiculosus against Oxidative and Transcriptional Responses in liver of sulfoxaflor exposed mice: assessment of DNA damage, DNA damage repair genes (XRCC1, OGG1, APE1, and PARP1) and antioxidant status

Abstract

This research aimed to determine regulatory role of sulfated polysaccharides fucoidan from Fucus vesiculosus against oxidative and transcriptional responses in liver of sulfoxaflor exposed mice liver. Fort this purpose both sulfoxaflor and fucoidan were given orally to mice for 24 hours and 7 days at doses of 15 mg/kg/day (equivalent to 1/50 oral LD50) and 50 mg/kg/day. At the end of the tests, liver samples were collected and used to assess 8-OHdG levels, the mRNA expression levels of DNA damage response genes such as XRCC1, OGG1, APE1, and PARP1. Furthermore, levels of tGSH and enzyme activity of GPx, GR, and GST, as well as TBARS, were also examined. The results showed that sulfoxaflor caused oxidative responses via increasing 8-OHdG and TBARS levels and altering antioxidant status in the liver. Furthermore, sulfoxaflor increased the mRNA expression of XRCC1 and APE1 genes, which are involved in the DNA repair mechanism. It was discovered that fucoidan protected liver cells against sulfoxaflor-induced DNA damage and lipid peroxidation by activating the tGSH and GPx, GR, GST. Additionally, it had a regulatory role in the liver of mice, affecting the mRNA expression of XRCC1 and APE1.