Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica

Cervantes-Chávez, José Antonio; Kronberg, Florencia; Passeron, Susana; Ruíz-Herrera, José

doi:10.1016/j.fgb.2009.02.005

Cited by 48 publications

(48 citation statements)

References 60 publications

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“…Based on these data, we speculate that Znc1p is most likely not involved in controlling the expression of HOY1 and MHY1 , which encode putative transcription factors, or the TPK1 gene, which encodes the catalytic subunit of PKA, because their expression levels increased in znc1::URA3 cells during the yeast-to-hypha transition. This result has previously been reported for wild-type cells [16], [17], [20]. The expression level of STE12 decreased in znc1::URA3 cells, and the expression level of BMH2 decreased in the same cell type during the dimorphic transition.…”

Section: Resultssupporting

confidence: 88%

“…The second group contains genes such as STE7 , STE11 , CLA4, BMH1 and RKA1 whose expression levels differ marginally between znc1::URA3 cells and wild-type cells, again suggesting a role for Znc1p in the regulation of their expression. The third group contains genes whose expression is unaffected by the deletion of ZNC1 ; these include the HOY1 and MHY1 genes that encode known transcription factors [16], [17], [20], the TPK1 gene that encodes the catalytic subunit of PKA [20], and the TUP1 gene that also encodes a transcription factor [42], [43].…”

Section: Discussionmentioning

confidence: 99%

“…In Y. lipolytica , the MAPK and PKA pathways have opposing actions in regulating dimorphism [20]. Mutants in the gene STE11, which encodes MAPKKK, grow constitutively in the yeast-like form [22], but cells with mutations in the TPK1 gene, which encodes the PKA catalytic subunit, grow constitutively in the mycelial form [20].…”

Section: Discussionmentioning

confidence: 99%

“…A number of genes involved in signal transduction are also involved in the dimorphic transition in Y. lipolytica . These include mutants of CLA4 , which are unable to form filaments or invade agar [19], TPK1 , which are constitutive for the mycelial form [20], RAC1 , which show alterations in cell morphology [21], and STE11 , which have lost the capacity to mate and to grow in the mycelial form [22]. A completely sequenced Y .…”

Section: Introductionmentioning

confidence: 99%

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Identification of the Transcription Factor Znc1p, which Regulates the Yeast-to-Hypha Transition in the Dimorphic Yeast Yarrowia lipolytica

et al. 2013

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The dimorphic yeast Yarrowia lipolytica is used as a model to study fungal differentiation because it grows as yeast-like cells or forms hyphal cells in response to changes in environmental conditions. Here, we report the isolation and characterization of a gene, ZNC1, involved in the dimorphic transition in Y. lipolytica. The ZNC1 gene encodes a 782 amino acid protein that contains a Zn(II)2C6 fungal-type zinc finger DNA-binding domain and a leucine zipper domain. ZNC1 transcription is elevated during yeast growth and decreases during the formation of mycelium. Cells in which ZNC1 has been deleted show increased hyphal cell formation. Znc1p-GFP localizes to the nucleus, but mutations within the leucine zipper domain of Znc1p, and to a lesser extent within the Zn(II)2C6 domain, result in a mislocalization of Znc1p to the cytoplasm. Microarrays comparing gene expression between znc1::URA3 and wild-type cells during both exponential growth and the induction of the yeast-to-hypha transition revealed 1,214 genes whose expression was changed by 2-fold or more under at least one of the conditions analyzed. Our results suggest that Znc1p acts as a transcription factor repressing hyphal cell formation and functions as part of a complex network regulating mycelial growth in Y. lipolytica.

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Section: Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of the Transcription Factor Znc1p, which Regulates the Yeast-to-Hypha Transition in the Dimorphic Yeast Yarrowia lipolytica

et al. 2013

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“…For example, (Rodriguez and Domínguez 1984) reported that elongation may operate only upon entering the stationary phase, and during the growth phase, Y. lipolytica may stay in yeast-like mode (Chen et al 1997; Makri et al 2010). The differences may be explained by the effect of temperature (Makri et al 2010) due to heat shock (Guevara-Olvera et al 1993), to the atmosphere composition and in the presence of specific compounds in the culture media (Barth and Gaillardin 1997; Cervantes-Chávez et al 2009), as well as the nature of carbon substrate (Rodriguez and Domínguez 1984; Guevara-Olvera et al 1993; Ruiz-Herrera and Sentandreu 2002), and to the atmosphere of growth media and pH (Ruiz-Herrera and Sentandreu 2002). Other studies (Barth and Gaillardin 1997; Zinjarde et al 1998) also suggest that the capacity of dimorphism is strain specific.…”

Section: Discussionmentioning

confidence: 99%

Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775

Bouchedja

Danthine

Kar

et al. 2017

Bioresour. Bioprocess.

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This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia lipolytica JMY775 and its lipids accumulation. The results obtained in this study stress forward that the use of an original tool, consisting of coupling bioreactors to online flow cytometry, is highly efficient. Throughout 48 h of culturing, this emerging process allowed an online continuous observation of the effects of pH and/or aeration on the cell growth and dimorphism and lipid accumulation by Y. lipolytica. This present study showed clearly that online flow cytometry is an advantageous tool for the real-time monitoring of microbial culture at a single-cell level. Indeed, the present investigation showed for the first time that profiling of the various phenomena and their monitoring upon culture time is now possible by coupling online cytometry with culture bioreactors.

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Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica

Liu,

Xia,

Chen

et al. 2024

Biotech & Bioengineering

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This study aimed to investigate the effect of hyphal formation in Yarrowia lipolytica and biochar addition on erythritol production by submerged fermentation. Hyphal formation significantly inhibited erythritol production by Y. lipolytica. Transcriptome analysis suggested that the impaired erythritol synthesis of hyphal cells was associated with the differential expression of genes involved in amino acid metabolism, lipid metabolism, and cell wall stability. Deletion of RAS2 responsible for yeast‐to‐hypha transition and EYD1 included in erythritol degradation blocked hyphal formation and improved erythritol production. Biochar prepared from corncob, sugarcane bagasse (SB), corn straw, peanut shell, coconut shell, and walnut shell (WS) had a positive effect on erythritol production, of which WS pyrolyzed at 500°C (WSc) performed the best in flask fermentation. In a 3.7 L bioreactor, 220.20 ± 10 g/L erythritol with a productivity of 2.30 ± 0.10 g/L/h was obtained in the presence of 1.4% (w/v) WSc and 0.7% SBc (SB pyrolyzed at 500°C) within 96 h. These results suggest that inhibition of hyphal formation together with biochar addition is an efficient way to promote erythritol production.

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Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica

Cited by 48 publications

References 60 publications

Identification of the Transcription Factor Znc1p, which Regulates the Yeast-to-Hypha Transition in the Dimorphic Yeast Yarrowia lipolytica

Identification of the Transcription Factor Znc1p, which Regulates the Yeast-to-Hypha Transition in the Dimorphic Yeast Yarrowia lipolytica

Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775

Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica

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