Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer

Meng, Xianhua; Zhao, Yinghui; Wang, Jin-Yun; Gao, Zheng; Geng, Qing-Xia; Liu, Xiaoxia

doi:10.3892/etm.2017.4887

Cited by 25 publications

(25 citation statements)

References 33 publications

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“…miR-758 is also underexpressed in cervical cancer tissues, blood cells and cervical exfoliated cells. The underexpression of miR-758 is strongly associated with the infiltration and invasion of cervical cancer (22). Therefore, miR-758 has potential applications for the diagnosis and treatment of patients with these specific cancer types.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, in-depth investigation of the detailed roles of miRNAs and their underlying mechanisms in TSCC is likely to provide therapeutic targets for the treatment of patients with this aggressive disease. miRNA (miR)-758 is a miRNA that has been frequently studied in non-small cell lung cancer (20), hepatocellular carcinoma (21) and cervical cancer (22). However, to date, the expression patterns and roles of miR-758 in TSCC have remained largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA‑758 inhibits tumorous behavior in tongue squamous cell carcinoma by directly targeting metadherin

Zhang

Zhao

2019

Mol Med Report

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Numerous microRNAs (miRNAs) are dysregulated in tongue squamous cell carcinoma (TSCC), and their dysregulation has been demonstrated to have a strong correlation with TSCC progression via regulation of their targets. Therefore, miRNAs have potential use in the diagnosis and treatment of patients with TSCC. In the present study, miRNA-758 (miR-758) expression in TSCC tissues and cell lines was detected through reverse transcription-quantitative polymerase chain reaction, and the effects of miR-758 on TSCC cell proliferation and invasion were investigated by using Cell Counting kit-8 and Transwell invasion assays. A luciferase reporter assay was performed to determine the target interaction between miR-758 and metadherin (MTDH) in TSCC cells. The results revealed that miR-758 was downregulated in TSCC tissues and cell lines. miR-758 overexpression restricted the proliferation and invasion of TSCC cells. Additionally, MTDH was verified as a direct target gene of miR-758 in TSCC cells. Furthermore, MTDH was observed to be upregulated in TSCC tissues, and the upregulation of MTDH was inversely correlated with miR-758 expression. Moreover, restored MTDH expression significantly counteracted the suppressive effects of miR-758 overexpression on TSCC cells. These results suggested that miR-758 may prevent TSCC progression and development by directly targeting MTDH, thereby providing evidence that miR-758 is a novel therapeutic target for the treatment of patients with TSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MicroRNA‑758 inhibits tumorous behavior in tongue squamous cell carcinoma by directly targeting metadherin

Zhang

Zhao

2019

Mol Med Report

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“…In particular, the overexpression of miR-758 was demonstrated to inhibit cell proliferation, migration, invasion and promote apoptosis in non-small cell lung cancer by negatively regulating HMGB (7). Meng et al also showed that miR-758 was downregulated in cervical cancer, and miR-758 overexpression promoted cell invasion by regulating the expression of MEPE (8). These results suggest that miR-758 can be stably expressed in human tissues.…”

Section: Introductionmentioning

confidence: 95%

MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway

Zhang¹,

Zhang

et al. 2020

Oncol Lett

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Recently, a number of microRNAs (miRNAs) have been reported to play different roles in human cancers, including colorectal cancer (CRC). However, the specific role of miR-758 has not been clarified in CRC. Therefore, the aim of the present study was to explore the role of miR-758 in CRC. RT-qPCR and western blot analysis were used to quantify the expression of miR-758 and genes. The function of miR-758 in CRC was investigated using Transwell, CCK-8 and luciferase reporter assays. According to the results, the downregulation of miR-758 expression was associated with aggressive behavior and poor prognosis in CRC patients. miR-758 was shown to restrain the cell viability and metastasis in CRC. In addition, it was confirmed that miR-758 directly targets PAX6 and inhibits CRC progression through targeting PAX6. The results also revealed that miR-758 blocked EMT and PI3K/AKT pathway in CRC. In conclusion, miR-758 acts as a tumor suppressor in CRC by downregulating PAX6.

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“…In cervical cancer, the expression of MEPE is closely associated with tumor metastasis and drug resistance. Meng et al showed that MEPE expression in human cervical cancer tissue was significantly different to that of normal cervical tissue [6]. Their study also suggested that MEPE expression is regulated by miR-758, a microRNA associated with some MDR proteins such as multidrug resistance-associated protein (MRP-1) and glutathione S-transferase π (GST-π) [7,8].…”

Section: Introductionmentioning

confidence: 99%

Mifepristone regulates the multidrug resistance via miR-758/MEPE of Hela cell lines

Hong¹,

Zhang²,

Liu³

et al. 2020

EJGO

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Background:In cervical cancer, matrix extracellular phosphoglycoprotein (MEPE) plays an important role of multidrug resistance, which is associated with miR-758. Mifepristone has anti-drug resistance effects, but whether mifepristone regulates the expression of multidrug resistance proteins via the pathway mediated by miR-758/MEPE is still unclear. Hela cells were induced by mifepristone (named as Hela/MIF cells). Then, matrix extracellular phosphoglycoprotein siRNA, and miR-758 mimics were transfected into HeLa/MIF cells. The sensitivity, clone forming ability, migration, and level of apoptosis of the cells in each group were respectively measured by CCK-8 assays, clone forming assays, transwell assays, and flow cytometry analysis. The targeting of miR-758 to MEPE was verified by dual-luciferase assay. At last, the expression of MDR-1, MRP-1, and GST-π were detected by qPCR and western blot assays. Results: The induction by mifepristone not only decreased the sensitivity of Hela cells, but also up-regulated the expression of multidrug resistance proteins. On this basis, by increasing the expression of miR-758 or downregulating the expression of MEPE, the expression of multidrug resistance proteins decreased, while the sensitivity of Hela cells to mifepristone were improved and the level of Hela cells proliferation, colony forming, and invasion further decreased. Conclusions: The induction by mifepristone inhibited the proliferation of Hela cells, but the sensitivity of Hela cells increased. The mechanism may depend on the expression of multidrug resistance protein. This study shows that regulating the expression of miR-758 and MEPE can reduce the resistance of Hela cells to mifepristone, enhance the sensitivity, and further improve the inhibitory effect of mifepristone.

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Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer

Cited by 25 publications

References 33 publications

MicroRNA‑758 inhibits tumorous behavior in tongue squamous cell carcinoma by directly targeting metadherin

MicroRNA‑758 inhibits tumorous behavior in tongue squamous cell carcinoma by directly targeting metadherin

MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway

Mifepristone regulates the multidrug resistance via miR-758/MEPE of Hela cell lines

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