Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures.

Barthels, Nathalie; Lee, F.M. van der; Klap, Joke C.; Goddijn, Oscar J. M.; Karimi, Mansour; Puzio, Piotr S.; Grundler, Florian M. W.; Ohl, Stephan; Lindsey, Keith; Robertson, Lee; Robertson, W. M.; Montagu, Marc Van; Gheysen, Godelieve; Sijmons, Peter C.

doi:10.1105/tpc.9.12.2119

Cited by 89 publications

(42 citation statements)

References 45 publications

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“…In order to identify host genes that are induced in response to Orobanche parasitism, we have screened promoter-trap lines of Arabidopsis generated by transforming Arabidopsis with a T-DNA containing a promoterless GUS construct close to the right border (Barthels et al, 1997) (Westwood, unpublished data). These lines have been screened for GUS expression in response to O. aegyptiaca using the PE bag method and at least 33 different lines expressing GUS have been identified.…”

Section: Post Attachment Interactions Between Orobanche and Arabidopsismentioning

confidence: 99%

The Use of Arabidopsis to Study Interactions between Parasitic Angiosperms and Their Plant Hosts

Goldwasser

Westwood

Yoder

2002

The Arabidopsis Book

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Parasitic plants invade host plants in order to rob them of water, minerals and nutrients. The consequences to the infected hosts can be debilitating and some of the world's most pernicious agricultural weeds are parasitic. Parasitic genera of the Scrophulariaceae and Orobanchaceae directly invade roots of neighboring plants via underground structures called haustoria. The mechanisms by which these parasites identify and associate with host plants present unsurpassed opportunities for studying chemical signaling in plant-plant interactions. Seeds of some parasites require specific host factors for efficient germination, thereby insuring the availability of an appropriate host root prior to germination. A second set of signal molecules is required to induce haustorium development and the beginning of heterotrophy. Later stages in parasitism also require the presence of host factors, although these have not yet been well characterized. Arabidopsis is being used as a model host plant to identify genetic loci associated with stimulating parasite germination, haustorium development, and parasite support. Arabidopsis is also being employed to explore how host plants respond to parasite attack. Current methodologies and recent findings in Arabidopsis - parasitic plant interactions will be discussed.

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Section: Post Attachment Interactions Between Orobanche and Arabidopsismentioning

confidence: 99%

The Use of Arabidopsis to Study Interactions between Parasitic Angiosperms and Their Plant Hosts

Goldwasser

Westwood

Yoder

2002

The Arabidopsis Book

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“…This approach has been successfully applied to genes that are difficult to identify by traditional methods. These include regulatory sequences that drive reporter gene expression in nematode feeding structures (Barthels et al, 1997), molecular markers for embryogenesis (Topping and Lindsey, 1997), regulatory sequences that mediate guard cell-specific expression (Plesch et al, 2000), enhancer trapped lines that show circadian-regulated expression (Michael and McClung, 2003), or senescence-associated expression (He et al, 2001).…”

Section: Entrapment Taggingmentioning

confidence: 99%

Reverse Genetic Approaches for Functional Genomics of Rice

Jeong

Jung

et al. 2005

Plant Mol Biol

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T-DNA and transposable elements e.g., Ds and Tos17, are used to generate a large number of insertional mutant lines in rice. Some carry the GUS or GFP reporter for gene trap or enhancer trap. These reporter systems are valuable for identifying tissue- or organ-preferential genes. Activation tagging lines have also been generated for screening mutants and isolating mutagenized genes. To utilize these resources more efficiently, tagged lines have been produced for reverse genetic approaches. DNA pools of the T-DNA tagged lines and Tos17 lines have been prepared for PCR screening of insertional mutants in a given gene. Tag end sequences (TES) of the inserts have also been produced. TES databases are beneficial for analyzing the function of a large number of rice genes.

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“…Cyst nematode J2 migrate intracellularly and root-knot J2 migrate intercellularly toward the vascular tissue. Here, an initial feeding cell is selected (Barthels et al, 1997). Secretions from the pharyngeal glands of the nematode are introduced into this initial feeding cell through the bore of the stylet, resulting in dramatic changes in gene expression and causing the cytological modifications that define a nematode feeding site.…”

Section: Biology Of Plant-parasitic Nematodesmentioning

confidence: 99%

Caenorhabditis elegans as a model for plant-parasitic nematodes

Costa

Lilley

Urwin

2007

Nematol

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Many studies on aspects of the biology of plant-parasitic nematodes can be facilitated by using the information and resources available for the model species Caenorhabditis elegans. Comparative genomics of shared processes can provide insights into plant-parasitic nematode biology that would otherwise be intractable. In this article we consider some of the resources available for C. elegans. We describe the practical utility of C. elegans and the use of available information to facilitate the characterisation of neurobiological processes in plant-parasitic nematodes.Model organisms, such as Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster and Arabidopsis thaliana, share a number of common characteristics, including a short life cycle, small adult size, easy maintenance in large numbers and tractability. Their simplicity of structure, in terms of both anatomy and genome organisation often lends itself readily to investigation (Bolker, 1995). Many basic biological processes are shared between model and target organisms. Having identified genes, proteins or processes of interest in a model organism it is then possible to study the target organism comparatively. Model organisms are often more amenable to technical manipulation than the target organisms. These factors lead to an increasing wealth of information and technical resources that pertain to certain model species. The free-living nematode, C. elegans, has become the model organism of choice both for other nematode species and for higher metazoans. The small size and obligate biotrophic life cycles of plant-parasitic nematodes make them refractory to many biological and genetic studies. Plant-parasitic nematodes and C. elegans share many similarities despite their disparate modes of existence. In certain areas of investigation, C. elegans can be used as a powerful model for the study of plantparasitic nematodes. Biology of plant-parasitic nematodesPlant-parasitic nematodes comprise more than 50 genera, primarily within the orders Tylenchida and Dory-

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Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures.

Cited by 89 publications

References 45 publications

The Use of Arabidopsis to Study Interactions between Parasitic Angiosperms and Their Plant Hosts

The Use of Arabidopsis to Study Interactions between Parasitic Angiosperms and Their Plant Hosts

Reverse Genetic Approaches for Functional Genomics of Rice

Caenorhabditis elegans as a model for plant-parasitic nematodes

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