Regulatory Signals and Tissue Interactions in the Early Hematopoietic Cell Differentiation in Xenopus laevis Embryo

Maéno, Mitsugu

doi:10.2108/zsj.20.939

Cited by 18 publications

(21 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5F. Different lines of evidence have demonstrated the role of ectodermally expressed BMP in regulating hematopoeisis in Xenopus embryos (22). Using explant combinations, we here show that BMP4 expression in the ectoderm is dependent on Wnt4 expression in the mesoderm.…”

Section: Discussionmentioning

confidence: 52%

Wnt/β-catenin signaling is involved in the induction and maintenance of primitive hematopoiesis in the vertebrate embryo

Tran

Sekkali

Imschoot

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The formation of primitive (embryonic) blood in vertebrates is mediated by spatio-temporally restricted signaling between different tissue layers. In Xenopus , in which primitive blood originates in the ventral blood island, this involves the secretion of bone morphogenetic protein (BMP) ligands by the ectoderm that signal to the underlying mesoderm during gastrulation. Using novel transgenic reporter lines, we report that the canonical Wnt/β-catenin pathway is also activated in the blood islands in Xenopus . Furthermore, Wnt-reporter activity was also detected in the blood islands of the mouse yolk sac. By using morpholino-mediated depletion in Xenopus , we identified Wnt4 as the ligand that is expressed in the mesoderm of the ventral blood island and is essential for the expression of hematopoietic and erythroid marker genes. Injection of an inducible Wnt-interfering construct further showed that, during gastrulation, Wnt/β-catenin signaling is required both in the mesoderm and in the overlying ectoderm for the formation of the ventral blood island. Using recombination assays with embryonic explants, we document that ectodermal BMP4 expression is dependent on Wnt4 signals from the mesoderm. Our results thus reveal a unique role for Wnt4-mediated canonical signaling in the formation and maintenance of the ventral blood island in Xenopus .

show abstract

Section: Discussionmentioning

confidence: 52%

Wnt/β-catenin signaling is involved in the induction and maintenance of primitive hematopoiesis in the vertebrate embryo

Tran

Sekkali

Imschoot

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…In amphibians, primitive blood cells, including larval RBCs, arise mostly from the ventral blood islands, 30 whereas definitive RBCs, including adult RBCs, are mainly derived from the dorsallateral plate mesoderm. During the neurula stages, the ventral blood islands and dorsal-lateral plate are committed to primitive or definitive hematopoiesis, respectively.…”

Section: Discussionmentioning

confidence: 99%

Tumor necrosis factor–related apoptosis-inducing ligand 1 (TRAIL1) enhances the transition of red blood cells from the larval to adult type during metamorphosis in Xenopus

Tamura

Mawaribuchi

Yoshimoto

et al. 2010

Blood

View full text Add to dashboard Cite

“…Bmps also function at later stages to prevent bipotential progenitors from choosing the endothelial rather than the erythroid lineage (Myers and Krieg, 2013). Bmps are believed to comprise part of the noncell-autonomous signal that is transmitted from the ectoderm to the mesoderm to enable it to form blood (Kumano et al, 1999;Maeno, 2003;Tran et al, 2010). Consistent with this idea, mesodermal progenitors of the anterior (a)VBI reside in a dorsal region of the embryo that is devoid of Bmp signaling at the onset of gastrulation, but then migrate beneath the ectoderm, which is rich in Bmps, before committing to an erythroid fate (Ciau-Uitz et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Tril targets Smad7 for degradation to allow hematopoietic specification in Xenopus embryos

et al. 2016

View full text Add to dashboard Cite

In Xenopus laevis, Bone morphogenetic proteins induce expression of the transcription factor Gata2 during gastrulation, and Gata2 is required in both ectodermal and mesodermal cells to enable mesoderm to commit to a hematopoietic fate. In the current studies, we identify tril as a Gata2 target gene that is required in both ectoderm and mesoderm for primitive hematopoiesis to occur. Tril is a transmembrane protein that functions as a co-receptor for Toll like receptors to mediate innate immune responses in the adult brain, but developmental roles for this molecule have not been identified. We show that Tril function is required both upstream and downstream of Bmp receptor mediated Smad1 phosphorylation for induction of Bmp target genes. Mechanistically, Tril triggers degradation of the Bmp inhibitor, Smad7. Tril-dependent down regulation of Smad7 relieves repression of endogenous Bmp signaling during gastrulation and this enables mesodermal progenitors to commit to a blood fate. Thus, Tril is a novel component of a Bmp-Gata2 positive feedback loop that plays an essential role in hematopoietic specification.

show abstract

Regulatory Signals and Tissue Interactions in the Early Hematopoietic Cell Differentiation in Xenopus laevis Embryo

Cited by 18 publications

References 64 publications

Wnt/β-catenin signaling is involved in the induction and maintenance of primitive hematopoiesis in the vertebrate embryo

Wnt/β-catenin signaling is involved in the induction and maintenance of primitive hematopoiesis in the vertebrate embryo

Tumor necrosis factor–related apoptosis-inducing ligand 1 (TRAIL1) enhances the transition of red blood cells from the larval to adult type during metamorphosis in Xenopus

Tril targets Smad7 for degradation to allow hematopoietic specification in Xenopus embryos

Contact Info

Product

Resources

About