Regulatory T Cell Modulation of Cytokine and Cellular Networks in Corneal Graft Rejection

Tahvildari, Maryam; Inomata, Takenori; Amouzegar, Afsaneh; Dana, Reza

doi:10.1007/s40135-018-0191-2

Cited by 18 publications

(16 citation statements)

References 114 publications

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“…IL-10 and TGF-β are important in tolerance induction and immune regulation, and IL-2 is involved in the development of Tregs. 41 IL-2 mediates its effects by binding to an IL-2 receptor that is highly expressed on Tregs and activated T cells, subsequently promoting Treg expansion. 41 , 42 These increased immunosuppressive cytokines may promote Treg expansion and maintenance in the inflammatory microenvironment, which may help corneal graft survival in vivo.…”

Section: Discussionmentioning

confidence: 99%

Ex Vivo–Induced Bone Marrow-Derived Myeloid Suppressor Cells Prevent Corneal Allograft Rejection in Mice

Zhu

Inomata

Fujimoto

et al. 2021

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To investigate the effects of ex vivo-induced bone marrow myeloid-derived suppressor cells (BM-MDSCs) on allogeneic immune responses in corneal transplantation. METHODS.Bone marrow cells from C57BL/6J (B6) mice were cultured with IL-6 and GM-CSF for four days. The ex vivo induction of the BM-MDSCs was assessed using flow cytometry, inducible nitric oxide synthase (iNOS) mRNA expression using reverse transcriptionquantitative polymerase chain reaction, and nitric oxide (NO) production in allogeneic stimulation. T-cell proliferation and regulatory T-cell (Treg) expansion were investigated on allogeneic stimulation in the presence of ex vivo-induced BM-MDSCs. IFN-γ , IL-2, IL-10, and TGF-β1 protein levels were measured using enzyme-linked immunosorbent assays. After subconjunctival injection of ex vivo-induced BM-MDSCs, the migration of the BM-MDSCs into corneal grafts, allogeneic corneal graft survival, neovascularization, and lymphangiogenesis were assessed using flow cytometry, slit-lamp microscopy, and immunohistochemistry. RESULTS. The combination of GM-CSF and IL-6 significantly induced BM-MDSCs with increased iNos mRNA expression. The ex vivo-induced BM-MDSCs promoted NO release in allogeneic stimulation in vitro. The ex vivo-induced BM-MDSCs inhibited T-cell proliferation and promoted Treg expansion. Decreased IFN-γ and increased IL-2, IL-10, and TGF-β1 production was observed in coculture of ex vivo-induced BM-MDSCs. Injected ex vivo-induced BM-MDSCs were confirmed to migrate into the grafts. The injected BM-MDSCs also prolonged corneal graft survival and prevented angiogenesis and lymphangiogenesis. CONCLUSIONS.The ex vivo-induced BM-MDSCs have suppressive effects on allogeneic immune responses and prolong corneal allograft survival via the iNOS pathway, indicating that they may be a potential therapeutic tool for corneal transplantation.

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Section: Discussionmentioning

confidence: 99%

Ex Vivo–Induced Bone Marrow-Derived Myeloid Suppressor Cells Prevent Corneal Allograft Rejection in Mice

Zhu

Inomata

Fujimoto

et al. 2021

Invest. Ophthalmol. Vis. Sci.

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“…The next question would be which cell type harboring the increased abundance of lncRNA 003946 mediates the suppressing effects of IL-10-BM-MSCs on corneal allograft rejection. In view of the causal role of APCs in corneal allograft rejection 40 , 41 , the graft-infiltrating and draining-lymph-node-residing CD68 + macrophages, whose number has been synergistically or additively suppressed by IL-10-BM-MSCs in comparison to rIL-10 or plain BM-MSCs, would be the best candidate. Indeed, the RNAscope in situ hybridization revealed that the expression of lncRNA 003946 was significantly upregulated in the CD68 + APCs infiltrating in the corneal allografts of the rIL-10 and RFP-BM-MSC groups, which was even enhanced in the IL-10-BM-BMC-treated corneal allografts.…”

Section: Discussionmentioning

confidence: 99%

Lentivirus-mediated IL-10-expressing Bone Marrow Mesenchymal Stem Cells promote corneal allograft survival via upregulating lncRNA 003946 in a rat model of corneal allograft rejection

Lu¹,

Ru²,

Chu³

et al. 2020

Theranostics

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Rationale: Corneal transplantation is an effective treatment to corneal blindness. However, the immune rejection imperils corneal allograft survival. An interventional modality is urgently needed to inhibit immune rejection and promote allograft survival. In our previous study, subconjunctival injections of bone marrow-derived mesenchymal stem cells (BM-MSCs) into a rat model of corneal allograft rejection extended allograft survival for 2 d. In this study, we sought to generate IL-10-overexpressing BM-MSCs, aiming to boost the survival-promoting effects of BM-MSCs on corneal allografts and explore the molecular and cellular mechanisms underlying augmented protection. Methods: A population of IL-10-overexpressing BM-MSCs (designated as IL-10-BM-MSCs) were generated by lentivirus transduction and FACS purification. The self-renewal, multi-differentiation, and immunoinhibitory capabilities of IL-10-BM-MSCs were examined by conventional assays. The IL-10-BM-MSCs were subconjunctivally injected into the model of corneal allograft rejection, and the allografts were monitored on a daily basis. The expression profiling of long noncoding RNA (lncRNA) in the allografts was revealed by RNA sequencing and verified by quantitative real-time PCR. The infiltrating immune cell type predominantly upregulating the lncRNA expression was identified by RNAscope in situ hybridization. The function of the upregulated lncRNA was proved by loss- and gain-of-function experiments both in vivo and in vitro . Results: The IL-10-BM-MSCs possessed an enhanced immunoinhibitory capability and unabated self-renewal and multi-differentiation potentials as compared to plain BM-MSCs. The subconjunctivally injected IL-10-BM-MSCs reduced immune cell infiltration and doubled allograft survival time (20 d) as compared to IL-10 protein or plain BM-MSCs in the corneal allograft rejection model. Further, IL-10-BM-MSCs significantly upregulated lncRNA 003946 expression in CD68 + macrophages infiltrating corneal allografts. Silencing and overexpressing lncRNA 003946 in macrophage cultures abolished and mimicked the IL-10-BM-MSCs' suppressing effects on the macrophages' antigen presentation, respectively. In parallel, knocking down and overexpressing the lncRNA in vivo abrogated and simulated the survival-promoting effects of IL-10-BM-MSCs on corneal allografts, respectively. Conclusion: The remarkable protective effects of IL-10-BM-MSCs support further developing them into an effective interventional modality against corneal allograft rejection. IL-10-BM-MSCs promote corneal allograft survival mainly through upregulating a novel lncRNA expression in graft-infiltrating CD68 + macrophages. LncRNA, for the first time, is integrated into an IL-10-BM-MSC-driven immunomodulatory axis against the immune rejection to corneal allograft.

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“…Tregs are a subset of CD4 + T cells with highly expressed CD4 + CD25 + surface m [72]; numerous studies have reported their immunosuppressive function in organ plantation. In this review, two papers including thirty-two cases were assessed [ The mean survival time was significantly prolonged in the Treg intervention group pared with that in the PBS control group, with a standard mean difference of 9.4 (95% CI, 4.14 to 14.70; P < 0.001) (Figure 5).…”

Section: Regulatory T Cells (Tregs) Promote Corneal Allograft Survivalmentioning

confidence: 99%

Role of Immune Cell Diversity and Heterogeneity in Corneal Graft Survival: A Systematic Review and Meta-Analysis

Zhu

Inomata

Zazzo

et al. 2021

JCM

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Corneal transplantation is one of the most successful forms of solid organ transplantation; however, immune rejection is still a major cause of corneal graft failure. Both innate and adaptive immunity play a significant role in allograft tolerance. Therefore, immune cells, cytokines, and signal-transduction pathways are critical therapeutic targets. In this analysis, we aimed to review the current literature on various immunotherapeutic approaches for corneal-allograft rejection using the PubMed, EMBASE, Web of Science, Cochrane, and China National Knowledge Infrastructure. Retrievable data for meta-analysis were screened and assessed. The review, which evaluated multiple immunotherapeutic approaches to prevent corneal allograft rejection, showed extensive involvement of innate and adaptive immunity components. Understanding the contribution of this immune diversity to the ocular surface is critical for ensuring corneal allograft survival.

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Regulatory T Cell Modulation of Cytokine and Cellular Networks in Corneal Graft Rejection

Cited by 18 publications

References 114 publications

Ex Vivo–Induced Bone Marrow-Derived Myeloid Suppressor Cells Prevent Corneal Allograft Rejection in Mice

Ex Vivo–Induced Bone Marrow-Derived Myeloid Suppressor Cells Prevent Corneal Allograft Rejection in Mice

Lentivirus-mediated IL-10-expressing Bone Marrow Mesenchymal Stem Cells promote corneal allograft survival via upregulating lncRNA 003946 in a rat model of corneal allograft rejection

Role of Immune Cell Diversity and Heterogeneity in Corneal Graft Survival: A Systematic Review and Meta-Analysis

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