Regulatory T cells (CD4+CD25brightFoxP3+) expansion in systemic sclerosis correlates with disease activity and severity

Slobodin, Gleb; Sheikh‐Ahmad, Mohammad; Rosner, Itzhak; Peri, Regina; Rozenbaum, Michael; Kessel, Aharon; Toubi, Elias; Odeh, Majed

doi:10.1016/j.cellimm.2009.12.009

Cited by 90 publications

(52 citation statements)

References 19 publications

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“…In SSc patients, few studies investigated the various Treg subsets and their suppressive function, these also with conflicting results, showing either reduced, 20-22 increased 23,24 or the same [25][26][27] number of circulating Treg cells in peripheral blood as compared with controls. These discrepancies might be mostly due to variations in study techniques, with setting analysis of either CD4 þ CD25 þ , 21,23 CD4 þ CD25 high , 12,25,26 20,24,26 CD25 þ Foxp3 þ CD127 -23 or CD4 þ CD25 high CD127 low subsets. 22 Despite these controversies, in most of these studies of SSc patients' samples, 22,23,26 impaired Treg-suppressive function was associated with SSc disease severity.…”

Section: Introductionmentioning

confidence: 99%

Peripheral blood regulatory T cells in patients with diffuse systemic sclerosis (SSc) before and after autologous hematopoietic SCT: a pilot study

Baraut

Grigore

Jean-Louis

et al. 2013

Bone Marrow Transplant

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The present pilot study aims to evaluate the frequency and the function of regulatory T (Treg) cells in patients with diffuse cutaneous SSc (dcSSc) before and after autologous hematopoietic SCT (aHSCT). Peripheral blood lymphocytes from seven dcSSc patients were analyzed before and 24 months after aHSCT and were compared with those from seven healthy donors (controls).Treg (aTreg) cell subsets was performed using four-color flow cytometry. Treg-suppressive capability was measured after coculture with autologous T effector cells by evaluation of T-cell proliferation using 3 H-thymidine incorporation. Peripheral CD4 þ CD25 high FoxP3 þ (2 ± 0.5 vs 4.2 ± 1.1, Po0.01), CD4 þ CD25 þ TGF-b þ (6.9 ± 1.8 vs 14.6 ± 5.0, Po0.05) and CD4 þ CD25 þ IL-10 þ (10.7 ± 0.5 vs 16.1±3.2, Po0.01) Tregs as well as CD4 þ CD25 high CD127 low Tregs suppressive capacity (Po0.05) were decreased in dcSSc patients vs controls. After aHSCT (n ¼ 7), the percentages of CD4 þ CD25 high FoxP3 þ (4.1 ± 1.8) and CD4 þ CD25 þ IL-10 þ (15.7 ± 2.2) Treg cells and the suppressive activity of CD4 þ CD25 high CD127 low were restored to the levels in controls. The decreased frequency and the functional defect of peripheral Treg cells from patients with dcSSc are reversed following aHSCT to reach those observed in controls. This pilot study brings evidence of an effective restoration of nTreg and aTreg subsets, and recovery of nTreg suppressive function following aHSCT.

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Section: Introductionmentioning

confidence: 99%

Peripheral blood regulatory T cells in patients with diffuse systemic sclerosis (SSc) before and after autologous hematopoietic SCT: a pilot study

Baraut

Grigore

Jean-Louis

et al. 2013

Bone Marrow Transplant

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“…According to another study, even though the absolute number of Tregs in peripheral blood of SSc patients did not differ from healthy controls, a smaller number of Tregs in SSc skin biopsies was found, which suggests suppressed immunomodulatory response [38]. Another study described an increased frequency of Tregs in peripheral blood of SSc patients and a correlation with disease activity was established [39]. Nonetheless, the Tregs expansion in the peripheral compartment was not associated with functional decline in the Treg population.…”

Section: Tregs Changes In Sscmentioning

confidence: 97%

T Helper Cells in the Immunopathogenesis of Systemic Sclerosis – Current Trends

Kurteva¹,

Kyurkchiev²

2017

Acta Medica Bulgarica

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“…The numbers of Tregs in the peripheral blood were increased, and correlated significantly with disease activity and severity. 58 By contrast, the number of FoxP3 + Tregs was fewer in the skin of patients with scleroderma. 59 The number of Tregs is increased, which however had a diminished capacity to control CD4 + effector T cells.…”

Section: O N O T D I S T R I B U T Ementioning

confidence: 98%