Regulatory volume decrease of rat kidney principal cells after successive hypo-osmotic shocks

Zarogiannis, Sotirios G.; Ilyaskin, A. V.; Baturina, G. S.; Katkova, L. E.; Медведев, Д. А.; Karpov, D. I.; Ершов, А. П.; Solenov, Evgeniy I.

doi:10.1016/j.mbs.2013.05.007

Cited by 21 publications

(26 citation statements)

References 35 publications

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“…On the basis of 3D confocal reconstructions in solutions of different osmolalities, the assumption that OMCD cell volume is proportional to cell height cubed could be accepted: V/V 0 = (H/H 0 ) 3 . Also, according to the results of Calcein calibration experiments on the OMCD principal cells (Zarogiannis et al 2013) and model simulations (unpublished data), we conclude that the apparent osmotically inactive portion of cell volume does not exceed 10% of the total cell volume. Taking into account that the osmotically inactive volume has a dynamic nature, it could not be determined precisely using an experimental approach.…”

Section: Calcein Fluorescence Calibrationsupporting

confidence: 66%

“…Calcein fluorescence was calibrated as it was previously described in detail (Zarogiannis et al 2013). In short, the Calcein fluorescence and cell height of superfused OMCD fragments were measured simultaneously when the cells were challenged by various osmolalities of the extracellular medium (150, 300, 400 and 600 mosmol/kgH 2 O).…”

Section: Calcein Fluorescence Calibrationmentioning

confidence: 99%

“…These experiments were described in our previous paper (Zarogiannis et al 2013). 3D confocal reconstructions were performed for cells balanced in solutions having different osmolalities (300, 400, 500 and 600 mosmol/kgH 2 O).…”

Section: Calcein Fluorescence Calibrationmentioning

confidence: 99%

“…3D confocal reconstructions were performed for cells balanced in solutions having different osmolalities (300, 400, 500 and 600 mosmol/kgH 2 O). Hypotonic medium caused rapid regulatory cell volume decrease (RVD) of these cells (Ilyaskin et al 2011;Zarogiannis et al 2013) and 3D confocal reconstruction in hypotonic solution was not performed. On the basis of 3D confocal reconstructions in solutions of different osmolalities, the assumption that OMCD cell volume is proportional to cell height cubed could be accepted: V/V 0 = (H/H 0 ) 3 .…”

Section: Calcein Fluorescence Calibrationmentioning

confidence: 99%

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Quantitative estimation of transmembrane ion transport in rat renal collecting duct principal cells

Ilyaskin

Karpov²,

Медведев³

et al. 2014

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Abstract. Kidney collecting duct principal cells play a key role in regulated tubular reabsorption of water and sodium and secretion of potassium. The importance of this function for the maintenance of the osmotic homeostasis of the whole organism motivates extensive study of the ion transport properties of collecting duct principal cells.We performed experimental measurements of cell volume and intracellular sodium concentration in rat renal collecting duct principal cells from the outer medulla (OMCD) and used a mathematical model describing transmembrane ion fluxes to analyze the experimental data. The sodium and chloride concentrations ([Na + ] in = 37.3 ± 3.3 mM, [Cl -] in = 32.2 ± 4.0 mM) in OMCD cells were quantitatively estimated. Correspondence between the experimentally measured cell physiological characteristics and the values of model permeability parameters was established. Plasma membrane permeabilities and the rates of transmembrane fluxes for sodium, potassium and chloride ions were estimated on the basis of ion substitution experiments and model predictions. In particular, calculated sodium (P Na ), potassium (P K ) and chloride (P Cl ) permeabilities were equal to 3.2 × 10 -6 cm/s, 1.0 × 10 -5 cm/s and 3.0 × 10 -6 cm/s, respectively. This approach sets grounds for utilization of experimental measurements of intracellular sodium concentration and cell volume to quantify the ion permeabilities of OMCD principal cells and aids us in understanding the physiology of the adjustment of renal sodium and potassium excretion.

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Section: Calcein Fluorescence Calibrationsupporting

confidence: 66%

Section: Calcein Fluorescence Calibrationmentioning

confidence: 99%

Section: Calcein Fluorescence Calibrationmentioning

confidence: 99%

Section: Calcein Fluorescence Calibrationmentioning

confidence: 99%

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Quantitative estimation of transmembrane ion transport in rat renal collecting duct principal cells

Ilyaskin

Karpov²,

Медведев³

et al. 2014

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“…[27][28][29] On this basis, cultured human embryonic kidney (HEK 293 Phoenix) cells have been chosen as a model to define whether heavy metals like Cd 2+ , deriving from environmental pollution, 4 may affect cell volume regulation under hyposmotic shock, a homeostatic parameter recognized to be essential for survival of many cells. 13,30 Being renal cells continuously exposed to physiological changes in the osmolarity of the external medium, 31 cell lines derived from kidney are a suitable model for monitoring homeostatic cell functions, such as the regulation of cellular volume, in the presence of xenobiotics. 32,33 Our results show that HEK 293 cells exhibit the expected OP within 4 min of 15% hyposmotic challenge, leading to water influx, as shown in other cell types.…”

Section: Discussionmentioning

confidence: 99%

Cadmium affects osmotic phase and regulatory volume decrease in cultured human embryonic kidney cells

et al. 2016

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The present investigation aims to verify whether cadmium (Cd 2+ ), a metal possibly accumulated in body tissues from air and food, affects cell volume regulation capability in cultured human embryonic kidney (HEK 293 Phoenix) cells. The osmotic phase (OP), which is the expected cell swelling due to aquaporins involvement after hyposmotic challenge, and regulatory volume decrease (RVD), bringing cell volume back to control values through Ca 2+ -dependent ion efflux (K + and Cl -), have been monitored in HEK 293 cells treated with Cd 2+ (1-10-100 µM) for different time intervals (30 min, 3 h, overnight) and then submitted to 15 % hyposmotic shock. The results show that both 1 and 10 µM Cd 2+ significantly reduced OP, whereas 100 µM impaired Cd 2+ RVD mechanisms. The use of glutathione (GSH, 200 µM) confirmed that Cd 2+ elicited its effect via oxidative damage, being RVD inhibition after Cd 2+ treatment prevented by this antioxidant compound. Our findings show that: i) HEK 293 cells are a suitable model to assay the effect of xenobiotics on cell homeostasis; ii) Cd 2+ , depending on its concentration, affects cell homeostasis at different levels, i.e. water and ion permeability, responsible for, respectively, OP and RVD mechanism, adding thus more information to the knowledge of Cd 2+ toxicology.

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Methods to Measure Water Permeability

Solenov

Baturina

Katkova

et al. 2017

Advances in Experimental Medicine and Biology

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Water permeability is a key feature of the cell plasma membranes and it has seminal importance for a number of cell functions such as cell volume regulation, cell proliferation, cell migration, and angiogenesis to name a few. The transport of water occurs mainly through plasma membrane water channels , the aquaporins, who have very important function in physiological and pathophysiological states. Due to the above the experimental assessment of the water permeability of cells and tissues is necessary. The development of new methodologies of measuring water permeability is a vibrant scientific field that constantly develops during the past three decades along with the advances in imaging mainly. In this chapter we describe and critically assess several methods that have been developed for the measurement of water permeability both in living cells as well as in tissues with a focus in the first category.

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Regulatory volume decrease of rat kidney principal cells after successive hypo-osmotic shocks

Cited by 21 publications

References 35 publications

Quantitative estimation of transmembrane ion transport in rat renal collecting duct principal cells

Quantitative estimation of transmembrane ion transport in rat renal collecting duct principal cells

Cadmium affects osmotic phase and regulatory volume decrease in cultured human embryonic kidney cells

Methods to Measure Water Permeability

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