Regulatory volume response of erythrocytes exposed to a gradual and slow decrease in medium osmolality

Godart, H; Ellory, J. Clive; Motais, R

doi:10.1007/s004240050845

Cited by 21 publications

(17 citation statements)

References 7 publications

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“…-Cl -cotransporter has been implicated with volume recovery in turbot hepatocytes (Michel et al 1994;Ollivier et al 2006) and trout proximal renal tubules (Kanli and Norderhus 1998). In fact, a number of reports for RBCs (Hoffmann et al 2009;Marshall 2010), including those for trout (Guizouarn et al 2000;Godart et al 1999), indicate erythrocytes rely on K ? -Cl -cotransport during RVD.…”

Section: Discussionmentioning

confidence: 96%

Regulatory volume response following hypotonic stress in Atlantic salmon erythrocytes

Wormser

Mason

Helm

et al. 2011

Fish Physiol Biochem

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The purpose of this study was to examine regulatory volume decrease (RVD) in Atlantic salmon red blood cells (RBCs). Osmotic fragility was determined optically, mean cell volume was measured electronically, and changes in intracellular Ca(2+) concentration were visualized using fluorescence microscopy and fluo-4-AM. Cells displayed an increase in osmotic fragility and an inhibition of volume recovery following hypotonic shock when they were exposed to a high taurine Ringer or when placed in a high K(+) medium. Interestingly, RVD in cells from fish collected during the summer depended more on taurine efflux, whereas fall cells relied more on the loss of K(+). In addition, RVD in fall cells was prevented with the K(+) channel inhibitor quinine, whereas the ionophore gramicidin decreased osmotic fragility and potentiated volume recovery. Further, hypotonic shock (0.5X Ringer) for both summer and fall cells caused an increase in cytosolic Ca(2+), which resulted from influx of this ion because it was not observed when extracellular Ca(2+) was chelated with EGTA (10 nM free Ca(2+)). Cells exposed to a low Ca(2+) hypotonic Ringer also had a greater osmotic fragility and failed to recover from hypotonic swelling. Finally, inhibition of phospholipase A(2) with ONO-RS-082 blocked volume recovery. In conclusion, Atlantic salmon RBCs displayed volume decrease in response to hypotonic shock, which depended on a swelling-induced influx of Ca(2+) and an increase in the efflux of K(+) and taurine.

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Section: Discussionmentioning

confidence: 96%

Regulatory volume response following hypotonic stress in Atlantic salmon erythrocytes

Wormser

Mason

Helm

et al. 2011

Fish Physiol Biochem

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show abstract

“…After this early report, IVR has been observed in the renal cell line A6, in the glioma cells C6 [69,70], in cerebellar granule neurons (unpublished), in cardiomyocytes [71] and in a more integrated preparation, the hippocampal slice [72]. In contrast, partial IVR is found in cardiomyocytes and no IVR is observed in trout erythrocytes [71,73] . The mechanisms subserving IVR have not been explored in detail.…”

Section: Isovolumetric Regulation: Role Of Amino Acidsmentioning

confidence: 99%

“…This may be related to features of taurine such as its metabolic inercy and its mainly cytoplasmic location, while GABA, glycine and glutamate, which have a prominent role as synaptic transmitters or are part of numerous metabolic cascades, may be sequestered in compartments which restrict their availability for osmosensitive release. Taurine efflux during IVR has been shown in cardiac myocytes and in trout erythrocytes, with reductions of 10-17% in taurine cell content [71,73]. About 30% in average, of the amino acid content in cells or slices is released during IVR.…”

Section: Isovolumetric Regulation: Role Of Amino Acidsmentioning

confidence: 99%

Amino Acid Osmolytes in Regulatory Volume Decrease and Isovolumetric Regulation in Brain Cells: Contribution and Mechanisms

Pasantes‐Morales

Franco

Torres-Márquez

et al. 2000

Cell Physiol Biochem

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Brain adaptation to hyposmolarity is accomplished by loss of both electrolytes and organic osmolytes, including amino acids, polyalcohols and methylamines. In brain in vivo, the organic osmolytes account for about 35% of the total solute loss. This review focus on the role of amino acids in cell volume regulation, in conditions of sudden hyposmosis, when cells respond by active regulatory volume decrease (RVD) or after gradual exposure to hyposmotic solutions, a condition where cell volume remains unchanged, named isovolumetric regulation (IVR). The amino acid efflux pathway during RVD is passive and is similar in many respects to the volume-activated anion pathway. The molecular identity of this pathway is still unknown, but the anion exchanger and the phospholemman are good candidates in certain cells. The activation trigger of the osmosensitive amino acid pathway is unclear, but intracellular ionic strength seems to be critically involved. Tyrosine protein kinases markedly influence amino acid efflux during RVD and may play an important role in the transduction signaling cascades for osmosensitive amino acid fluxes. During IVR, amino acids, particularly taurine are promptly released with an efflux threshold markedly lower than that of K⁺, emphasizing their contribution (possibly as well as of other organic osmolytes) vs inorganic ions, in the osmolarity range corresponding to physiopathological conditions. Amino acid efflux also occurs in response to isosmotic swelling as that associated with ischemia or trauma. Characterization of the pathway involved in this type of swelling is hampered by the fact that most osmolyte amino acids are also neuroactive amino acids and may be released in response to stimuli concurrent with swelling, such as depolarization or intracellular Ca⁺⁺ elevation.

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“…Neither K + nor taurine fluxes were activated in these cells which explains the absence of the volume regulatory response. 6 Hippocampal slices 7 exposed to an osmotic gradient of -2.5 mOsml/min, are also able to maintain a constant volume. In this preparation, no significant change in K + was found during IVR.…”

Section: Mechanism Of Isovolumetric Regulationmentioning

confidence: 99%

Isovolumetric Regulation in Mammal Cells: Role of Taurine

Ordaz¹,

Franco²,

Tuz³

2003

Advances in Experimental Medicine and Biology

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Regulatory volume response of erythrocytes exposed to a gradual and slow decrease in medium osmolality

Cited by 21 publications

References 7 publications

Regulatory volume response following hypotonic stress in Atlantic salmon erythrocytes

Regulatory volume response following hypotonic stress in Atlantic salmon erythrocytes

Amino Acid Osmolytes in Regulatory Volume Decrease and Isovolumetric Regulation in Brain Cells: Contribution and Mechanisms

Isovolumetric Regulation in Mammal Cells: Role of Taurine

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