Regulon Studies and
            <i>In Planta</i>
            Role of the BraI/R Quorum-Sensing System in the Plant-Beneficial Burkholderia Cluster

Coutinho, Bruna Gonçalves; Mitter, Birgit; Talbi, Chouhra; Sessitsch, Angela; Bedmar, Eulogio J.; Halliday, Nigel; James, Euan K.; Cámara, Miguel; Venturi, Vittorio

doi:10.1128/aem.00635-13

Cited by 25 publications

(19 citation statements)

References 56 publications

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“…The BraI/R QS system was found to be upregulated in the presence of rice plant macerate in the RNAseq experiment. Transcriptome studies have been performed to identify the BraI/R regulon in other closely related species of plant-beneficial Burkholderia spp., highlighting the fact that it is species-specific and no core regulon is present (Coutinho et al 2013a). Currently, the BraI/R regulon of B. kururiensis M130 is unknown and it is likely that it regulates loci that play important roles in endophytism.…”

Section: Discussionmentioning

confidence: 99%

Plant-Influenced Gene Expression in the Rice Endophyte Burkholderia kururiensis M130

Coutinho¹,

Licastro²,

Mendonça-Previato³

et al. 2015

MPMI

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113

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Burkholderia kururiensis M130 is one of the few rice endophytic diazotrophic bacteria identified thus far which is able to enhance growth of rice. To date, very little is known of how strain M130 and other endophytes enter and colonize plants. Here, we identified genes of strain M130 that are differentially regulated in the presence of rice plant extract. A genetic screening of a promoter probe transposon mutant genome bank and RNAseq analysis were performed. The screening of 10,100 insertions of the genomic transposon reporter library resulted in the isolation of 61 insertions displaying differential expression in response to rice macerate. The RNAseq results validated this screen and indicated that this endophytic bacterium undergoes major changes in the presence of plant extract regulating 27.7% of its open reading frames. A large number of differentially expressed genes encode membrane transporters and secretion systems, indicating that the exchange of molecules is an important aspect of bacterial endophytic growth. Genes related to motility, chemotaxis, and adhesion were also overrepresented, further suggesting plant–bacteria interaction. This work highlights the potential close signaling taking place between plants and bacteria and helps us to begin to understand the adaptation of an endophyte in planta.

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Section: Discussionmentioning

confidence: 99%

Plant-Influenced Gene Expression in the Rice Endophyte Burkholderia kururiensis M130

Coutinho¹,

Licastro²,

Mendonça-Previato³

et al. 2015

MPMI

Self Cite

113

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“…In the same way, endophytic colonization, germination, and plant-growth promotion of maize by Burkholderia phytofi rmans PsJN is not impacted in a strain defi cient in AHL production (Coutinho et al 2013 ). On the contrary, the BraIR QS system of Burkholderia kuriensis has been shown to be important for endophytic colonization of rice (SuarezMoreno et al 2010 ).…”

Section: Screening Of Qs Regulated Functions By Phenotypic Assaysmentioning

confidence: 96%

“…These global omics approaches revealed that QS can affect several percent of the protein or gene content in bacteria, as observed for B. phymatum or Rhizobium leguminosarum (Coutinho et al 2013 ;Cantero et al 2006 ).…”

Section: Omics Approaches To Identify Qs-regulated Genesmentioning

confidence: 97%

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Cell–Cell Communication in Azospirillum and Related PGPR

Wisniewski‐Dyé¹,

Vial²

2015

Handbook for Azospirillum

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Quorum-sensing (QS) regulation based on N -acyl-L -homoserine lactones (AHL) is used to control various phenotypes that are often essential for interaction with a eukaryotic host, notably for plant-associated bacteria. Technical methodologies currently used to reveal AHL production in a specifi c strain and to decipher phenotypes under QS regulation are surveyed in this chapter. Analyses conducted on the genus Azospirillum and on other related PGPR are used to illustrate the different steps of the approach. Among the genus Azospirillum , a survey of 40 strains belonging to six species revealed AHL production for four strains belonging to the lipoferum species or to a close undefi ned species and isolated from a rice rhizosphere. Identifi cation of genes mediating QS and regulating functions indicate that (1) distinct QS networks are present in some strains and seem to have been acquired independently by horizontal gene transfer; (2) QS regulation is strain specifi c with several phenotypes and numerous proteins being regulated by AHL-based QS in A. lipoferum B518, whereas no change is observed in A. lipoferum TVV3 defi cient in AHL production; and (3) QS is dedicated to regulating functions linked to rhizosphere competence and adaptation to plant roots in A. lipoferum B518.

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“…AHL molecule secreted from B. Phytofirmans PsJN affects root colonization and plant growth by auxin signaling in plants [12]. Coutinho and coworkers in 2013 identified 3-oxo-dodecanoyl homoserine lactone is expressed in highest amount among the different 3-oxo-AHLs produced by the BraI/R system of B. phytofirmans PsJN [13]. To identify the binding site of ARR10 of Arabidopsis thaliana with most expressed AHL, in our present work we dock ligand 3-oxo-dodecanoyl homoserine lactone with that protein.…”

Section: Introductionmentioning

confidence: 99%

Molecular Docking Analysis of AHL Molecule on Plant Protein ARR10

Basu¹,

Sarkar²

2015

Advances in Intelligent Systems and Computing

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In rhizosphere Plant Growth Promoting Rhizobacteria (PGPR) produce N-acyl-l-homoserine lactones (AHL) as the quorum-sensing (QS) signals. AHLs can act as trans-kingdom signalling molecules between plants and rhizobacteria and that can regulate plant growth and development. The plant-beneficial PGPR Burkholderia phytofirmans PsJN promotes growth in Arabidopsis thaliana by producing 3-oxo-dodecanoyl homoserine lactone (oxo-C14-HSL) from their quorum-sensing (QS) system. In bacteria, QS system functions by binding AHL to the LuxR-family of sensor/regulator proteins through their response regulator receiver domain. It has been hypothesized that by using similar response domain, Arabidopsis response regulator 10 (ARR10) proteins may act as binding site for 3-oxo-dodecanoyl homoserine lactone. ARRs are involved in cytokinin signalling pathways and thus these types of lactones can regulate growth in Arabidopsis. We prove the binding of oxo-C14-HSL with ARR10 by using molecular docking technique and analysing the docking result.

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Regulon Studies and In Planta Role of the BraI/R Quorum-Sensing System in the Plant-Beneficial Burkholderia Cluster

Cited by 25 publications

References 56 publications

Plant-Influenced Gene Expression in the Rice Endophyte Burkholderia kururiensis M130

Plant-Influenced Gene Expression in the Rice Endophyte Burkholderia kururiensis M130

Cell–Cell Communication in Azospirillum and Related PGPR

Molecular Docking Analysis of AHL Molecule on Plant Protein ARR10

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