Reidentification of Pestalotiopsis sensu lato causing gray blight of tea in Japan

Nozawa, S.; Togawa, Masayuki; Watanabe, Kyoko

doi:10.1007/s10327-022-01074-5

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Previous studies showed that a single ITS gene could be used to identify Pestalotiopsis microspora in H. brasiliensis (Kusdiana et al 2020), Oil Palm (Ismail et al 2017, and Adenium obesum (Jesus et al 2022). The combination of ITS, β-tubulin, and tef1 gene regions was reported to be a better taxonomic marker for Pestalotiopsis than a single gene (Maharachchikumbura et al 2016;Chen et al 2020;Das et al 2021;Aliya et al 2022a;Fiorenza et al 2022;Nozawa et al 2022).…”

Section: Discussionmentioning

confidence: 99%

New leaf fall disease in rubber-pathogen characterization and rubber clone resistance evaluation using detached leaf assay

DAROJAT,

ARDHIE,

OKTAVIA

et al. 2023

Biodiversitas

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Abstract. Darojat MR, Ardhie SW, Oktavia F, Sudarsono. 2023. New leaf fall disease in rubber-pathogen characterization and rubber clone resistance evaluation using detached leaf assay. Biodiversitas 24: 1935-1945. Leaf fall disease (LFD) has become a significant issue for rubber plantations worldwide. Over the last four years, a newly emerging LFD has posed an alarming problem in natural rubber production. The most efficient way to control LFD is to use resistance rubber clones. Therefore, this study aims to characterize the pathogen causing the newly emerging LFD and evaluate rubber clone resistance to the pathogen using detached-leaf assay. The fungal pathogens were isolated from 32 F1 progenies of PB 260 x SP 217 crosses, and the fungi were characterized and identified based on their morphological and molecular characteristics. The results showed that the isolated pathogen causing LFD was Neopestalotiopsis sp. Although they were all pathogenic, the arrays of isolated fungi exhibited various degrees of virulence, and P-212 was the most virulent fungal isolate. The resistance evaluation showed that rubber clones, isolates, and rubber clones by isolate interactions had a significant (p<0.05) effect on the lesion-symptom diameters. Based on the lesion diameter responses, the IRR 112 and RRIC 100 rubber clones were resistant to Pestalotiopsis sp. since they only showed less than 10 mm lesion diameters. The IRR 39 and PB 260 rubber clones were susceptible and showed more than 20 mm lesion diameters. The detached-leaf assay can easily screen rubber clones' responses to the fungi causing LFD. The resistance evaluation results can assist future rubber breeding strategies for the newly emerged LFD-resistant characters.

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Section: Discussionmentioning

confidence: 99%

New leaf fall disease in rubber-pathogen characterization and rubber clone resistance evaluation using detached leaf assay

DAROJAT,

ARDHIE,

OKTAVIA

et al. 2023

Biodiversitas

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“…It produces conidia and is a common plant pathogen causing various diseases, including canker, shoot blight, leaf spot, gray spot, leaf blight, and fruit rot [8][9][10][11][12][13]. P. sensulato was identified as the pathogen causing the gray blight of tea in Japan [14]. Additionally, P. theae has been linked to leaf spots in Ixora chinensis, and Elaeis guineensis [15,16].…”

Section: Introductionmentioning

confidence: 99%

The Identification and Role of the Key Mycotoxin of Pestalotiopsis kenyana Causing Leaf Spot Disease of Zanthoxylum schinifolium

Liu,

Li,

Chen

et al. 2023

JoF

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Leaf spot is a common disease of Zanthoxylum schinifolium (Z. schinifolium), which can seriously harm the plant’s ability to grow, flower, and fruit. Therefore, it is important to identify the mechanism of leaf spot caused by Pestalotiopsis kenyana (P. kenyana) for thorough comprehension and disease control. In this study, to verify whether the mycotoxins produced by P. kenyana cause leaf spot disease, the best medium for P. kenyana, namely PDB, was used. The mycotoxins were determined by ammonium sulfate precipitation as non-protein substances. The crude mycotoxin of P. kenyana was prepared, and the optimal eluent was eluted with petroleum either/ethyle acetate (3:1, v/v) and purified by silica gel column chromatography and preparative high-performance liquid chromatography to obtain the pure mycotoxins PK-1, PK-2, and PK-3. The PK-3 had the highest toxicity to Z. schinifolium, which may be the primary mycotoxin, according to the biological activity test using the spray method. The physiological and biochemical indexes of Z. schinifolium plants treated with PK-3 mycotoxin were determined. Within 35 days after mycotoxin treatment, the results showed that the protein content and malondialdehyde content of leaves increased over time. The soluble sugar and chlorophyll content decreased over time. The superoxide dismutase activity and catalase activity of the leaves increased first and then decreased, and the above changes were the same as those of Z. schinifolium inoculated with the spore suspension of the pathogen. Therefore, it is believed that the mycotoxin pestalopyrone could be a virulence factor that helps P. kenyana induce the infection of Z. schinifolium. In this study, the pathogenic mechanism of Z. schinifolium leaf spot was discussed, offering a theoretical foundation for improved disease prevention and control.

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Morphological and molecular reassessment of Pseudopestalotiopsis in the gray blight complex of tea with four new species from Sri Lanka

Weerasekara,

Udayanga,

Manamgoda

et al. 2024

Mycol Progress

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Reidentification of Pestalotiopsis sensu lato causing gray blight of tea in Japan

Cited by 5 publications

References 16 publications

New leaf fall disease in rubber-pathogen characterization and rubber clone resistance evaluation using detached leaf assay

New leaf fall disease in rubber-pathogen characterization and rubber clone resistance evaluation using detached leaf assay

The Identification and Role of the Key Mycotoxin of Pestalotiopsis kenyana Causing Leaf Spot Disease of Zanthoxylum schinifolium

Morphological and molecular reassessment of Pseudopestalotiopsis in the gray blight complex of tea with four new species from Sri Lanka

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