Reinforced Pericardium as a Hybrid Material for Cardiovascular Applications

Bracaglia, Laura G.; Li, Yu; Hibino, Narutoshi; Fisher, John P.

doi:10.1089/ten.tea.2014.0516

Cited by 25 publications

(48 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Poly(propylene fumarate) polymer is a photocrosslinkable, biocompatible and resorbable biomaterial which can be 3-D printed into almost any desirable shape and size and can be rendered with high mechanical strength [33, 34]. PPF has also been extensively studied for bone tissue engineering applications [33, 35–38], though, the vascularization of these scaffolds is less studied [39–43]. Fibrin, which is formed naturally in the body during wound healing [44], is widely used to enhance vascularization due to its pro-angiogenic properties.…”

Section: Introductionmentioning

confidence: 99%

Effect of prevascularization on in vivo vascularization of poly(propylene fumarate)/fibrin scaffolds

et al. 2016

Self Cite

View full text Add to dashboard Cite

The importance of vascularization in the field of bone tissue engineering has been established by previous studies. The present work proposes a novel poly(propylene fumarate) (PPF)/fibrin composite scaffold for the development of vascularized neobone tissue. The effect of prevascularization (i.e., in vitro pre-culture prior to implantation) with human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs) on in vivo vascularization of scaffolds was determined. Five conditions were studied: no pre-culture (NP), 1 week preculture (1P), 2 week pre-culture (2P), 3 week pre-culture (3P), and scaffolds without cells (control, C). Scaffolds were implanted subcutaneously in a severe combined immunodeficiency (SCID) mice model for 9 days. During in vitro studies, CD31 staining showed a significant increase in vascular network area over 3 weeks of culture. Vascular density was significantly higher in vivo when comparing NP to 3P groups. Immunohistochemical staining of human CD-31 expression indicated spreading of vascular networks with increasing pre-culture time. These vascular networks were perfused with mouse blood indicated by perfused lectin staining in human CD-31 positive vessels. Our results demonstrate that in vitro prevascularization supports in vivo vascularization in PPF/fibrin scaffolds.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of prevascularization on in vivo vascularization of poly(propylene fumarate)/fibrin scaffolds

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Despite their advantages, deterioration and calcification limit the lifespan of bioprosthetic substitutes to less than 10 years [7]. Furthermore, the calcification process in children is accelerated compared with adults, and bioprosthetics have a much shorter lifespan in young patients [8].…”

Section: Introductionmentioning

confidence: 99%

Comparison of physical and biological properties of CardioCel® with commonly used bioscaffolds

Neethling

Puls

Rea

2018

Interactive CardioVascular and Thoracic Surgery

View full text Add to dashboard Cite

OBJECTIVES: Durability of bioscaffolds cross-linked with glutaraldehyde and used in cardiovascular surgery is limited by biomechanical instability, calcification and reduced biocompatibility. This study compares CardioCel V R , a bovine pericardial scaffold engineered via the ADAPT V R process to ensure optimized biostability and biocompatibility, with the commonly used bioscaffolds. METHODS:Bovine pericardial scaffolds, cross-linked with 0.6% glutaraldehyde (XenoLogiX TM , PeriGuard V R ), dye-mediated photo-oxidized (PhotoFix TM ) and a non-crosslinked porcine scaffold (CorMatrix V R ), were compared with CardioCel (decellularized, cross-linked with 0.05% monomeric glutaraldehyde, detoxified) by thermal stability and mechanical tests. Biocompatibility and calcification were assessed in a juvenile subcutaneous rat model at 6 and 12 weeks. RESULTS:CardioCel displayed significantly higher (P < 0.01) cross-link stability (77.99 ± 0.64 C) than CorMatrix (57.88 ± 0.22 C) and PhotoFix (53.96 ± 0.41 C). Tensile strength of CardioCel (8.31 ± 3.36 MPa) was comparable with XenoLogiX (11.00 ± 5.43 MPa, P = 0.734), PeriGuard (16.44 ± 6.69 MPa, P = 0.136), PhotoFix (7.10 ± 6.11, P = 0.399) and CorMatrix (9.75 ± 2.61, P = 0.204). XenoLogiX and PeriGuard recorded the highest Young's modulus (67.01 ± 30.36 vs 95.67 ± 45.91 MPa), while CardioCel (50.21 ± 19.92 MPa) was comparable with CorMatrix (36.78 ± 10.47 MPa, P = 0.204) and PhotoFix (33.50 ± 10.24, P = 0.399). CorMatrix displayed a significantly (P < 0.05) greater stiffness (4.74 ± 0.77 MPa) at 10% strain than PeriGuard (3.73 ± 1.79 MPa), PhotoFix (1.59 ± 0.40 MPa) and CardioCel (3.39 ± 0.83 MPa). Differences in extractable calcium did not reach significance; however, the inorganic phosphorus content of PhotoFix (21.3 ± 9.0 mg/mg) was higher than CardioCel (11.35 ± 0.76 mg/mg, P = 0.004) or PeriGuard (10.7 ± 2.18 mg/mg, P = 0.002) at 12 weeks. CardioCel underwent a typical mild host-graft response with fibroblast infiltration and remodelling. Foreign body reactions were visible in both XenoLogiX and PeriGuard, with isolated fibroblast infiltration. PhotoFix showed severe inflammation and 2 implants were completely degraded at 12 weeks.CONCLUSIONS: CardioCel demonstrated optimized physical properties, minimal mineralization potential and superior biocompatibility. These results may benefit the long-term performance of this bioscaffold for cardiovascular surgery. The favourable characteristics of the comparator products were counterbalanced by less desirable features that may have negative implications on durability and performance when used in cardiovascular procedures.

show abstract

“…We propose a hybrid scaffold, combining ECM together with a synthetic polymer, as a strategic approach to control the signals from ECM that would affect polarization and inflammation. We have previously reported on the biohybrid material, poly(propylene fumarate) (PPF)‐reinforced pericardium, as a scaffold for vascular wall repair . This material combines the bioactivity of pericardium, a matrix‐dense tissue found surrounding the heart muscle, with the strength of the polymer PPF.…”

Section: Introductionmentioning

confidence: 99%

“…More importantly, the polymer reinforcement results in significantly lower calcification of the pericardium compared to the GA crosslinked pericardium in a rat subdermal implant after only 3 weeks. Changes in calcification could be explained by unnatural healing in response to eliminated or masked active components of the pericardium due to chemical cross‐linking . In addition, the PPF‐reinforced tissue hosted moderate cell infiltration, indicating a “middle road” between rapid scaffold deterioration in the untreated case, and capsule formation around the GA pericardium .…”

Section: Introductionmentioning

confidence: 99%

“…These results suggest that the PPF layer could be responsible for controlling the presentation of the potent ECM molecules to the system, allowing for the benefits of bioactive components, but eliminating the risk of rapid degradation and propagation of inflammatory cycles. This theory was developed based off of macrophage quantification (using immunostaining of F4/80, representing a general macrophage marker), and warrants a comprehensive investigation of inflammatory reaction to the material.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synthetic polymer coatings diminish chronic inflammation risk in large ECM‐based materials

Bracaglia

Winston

Powell

et al. 2018

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Degradable materials that can support cell infiltration and remodeling are the basis of tissue engineered approaches to vascular repair. In addition, to replace or close a large area of the vasculature, a patch material or scaffold must also withstand high pressure over time. Extracellular matrix‐based (ECM‐based) scaffolds offer a biological substrate with environmental cues that can support the formation of appropriate vascular tissue. However, scaffolds made from pure natural materials can degrade rapidly, resulting in reduced mechanical integrity of the implant and possible chronic inflammation in the site. A hybrid biomaterial, combining the matrix‐dense tissue pericardium with a layer of the degradable polymer poly(propylene fumarate) (PPF), is suited to withstand rapid enzymatic degradation and control the presentation of an unaltered natural tissue matrix for remodeling activity. In this study, we show that the polymer reinforced hybrid supports cellular infiltration, but has fewer macrophages in the vicinity of the implant after 6 weeks in vivo than an untreated tissue control in both athymic and immunocompetent rat models. This result is supported by changes seen in other inflammatory cell populations. Based on significant differences in the inflammatory response to untreated pericardium and PPF‐reinforced pericardium, we conclude that the polymer reinforcement layer can be used as a tool to leverage presentation of the ECM molecules in ECM‐based scaffolds. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 494–504, 2019.

show abstract

Reinforced Pericardium as a Hybrid Material for Cardiovascular Applications

Cited by 25 publications

References 24 publications

Effect of prevascularization on in vivo vascularization of poly(propylene fumarate)/fibrin scaffolds

Effect of prevascularization on in vivo vascularization of poly(propylene fumarate)/fibrin scaffolds

Comparison of physical and biological properties of CardioCel® with commonly used bioscaffolds

Synthetic polymer coatings diminish chronic inflammation risk in large ECM‐based materials

Contact Info

Product

Resources

About