In this article, polymer/graphene nanocomposite has been focused with reference to their application in biosensor. In this regard, various type of biosensorsbased on polymer/graphene nanocomposite has been explored. Major categories of biosensors studied include electrochemical biosensor, flexible biosensor, field effect biosensor, and glucose biosensor. Owing to excellent electrical, mechanical, thermal, and biomedical features, polymer/graphene nanocomposite has been found to improve the performance of different type of biosensors.