Rejuvenation and Freezing of Outdated Human Red Cells

Valeri, C. R.; Zaroulis, Charles G.

doi:10.1007/978-1-4684-3222-0_35

Cited by 8 publications

(8 citation statements)

References 30 publications

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“…Both warm (37°C) and cold (4°C) rejuvenation have been used, with warm rejuvenation more extensively studied than cold. Warm rejuvenation has been shown to improve RBC morphology and reduce RBC:endothelial cell interactions, and rejuvenated RBCs have good posttransfusion recovery and oxygen delivery 15‐17. It has also been shown that warm rejuvenation on Day 29 of storage can improve RBC deformability .…”

mentioning

confidence: 99%

Rejuvenation of ATP during storage does not reverse effects of the hypothermic storage lesion

2013

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confidence: 99%

Rejuvenation of ATP during storage does not reverse effects of the hypothermic storage lesion

2013

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“…38,39 It has also been reported that as many as half of hospitalized patients have hypophosphatemia after major surgery, 40 perhaps due to poor dietary intake, which may occur in the perioperative setting, 41 or due to renal wasting of phosphate. 41 It is possible, therefore, that the relatively slow recovery of 2,3-DPG after stored blood transfusions in our study could be explained in part by abnormal phosphate homeostasis.…”

Section: Discussionmentioning

confidence: 99%

2,3-Diphosphoglycerate Concentrations in Autologous Salvaged Versus Stored Red Blood Cells and in Surgical Patients After Transfusion

Scott

Nagababu

Johnson

et al. 2016

Anesthesia &Amp; Analgesia

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Background Stored red blood cells (RBCs) are deficient in 2,3-diphosphoglycerate (2,3-DPG), but it is unclear how autologous salvaged blood (ASB) compares to stored blood, and how rapidly 2,3-DPG levels return to normal after transfusion. Therefore, we compared levels of 2,3-DPG in stored versus ASB RBCs, and in patients’ blood following transfusion. Methods Twenty-four patients undergoing multilevel spine fusion surgery were enrolled. We measured 2,3-DPG and the oxyhemoglobin dissociation curve (P50) in samples taken from the ASB and stored blood-bags before transfusion, and in blood samples drawn from patients before and after transfusion. Results The mean storage duration for stored RBCs was 24 ± 8 days. Compared with fresh RBCs, stored RBCs had decreased 2,3-DPG levels (by ≈ 90%; P<0.0001), and a decreased P50 (by ≈ 30%; P<0.0001). However, ASB RBCs did not exhibit these changes. The mean 2,3-DPG concentration decreased by ≈ 20% (P<0.05) in postoperative blood sampled from patients who received 1 – 3 stored RBC units, and by ≈ 30% (P<0.01) in those who received ≥4 stored RBC units. 2,3-DPG was unchanged in patients who received no stored blood or ASB alone. After surgery, 2,3-DPG levels recovered gradually over three postoperative days in patients who received stored RBCs. Conclusions Stored RBCs, but not ASB RBCs, have decreased levels of 2,3-DPG and a left shift in the oxyhemoglobin dissociation curve. Postoperatively, 2,3-DPG levels remain below preoperative baseline levels for up to three postoperative days in patients who receive stored RBCs, but are unchanged in those who receive only ASB RBCs.

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“…While the effects of warm RBC rejuvenation and rejuvenation before RBC cryopreservation have been investigated quite thoroughly, less is known about rejuvenation during 42‐day HS. To examine this and maintain the RBCs' temperature during the procedure, a cold rejuvenation was chosen for this study.…”

Section: Discussionmentioning

confidence: 99%

The effects of rejuvenation during hypothermic storage on red blood cell membrane remodeling

et al. 2013

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Rejuvenation and Freezing of Outdated Human Red Cells

Cited by 8 publications

References 30 publications

Rejuvenation of ATP during storage does not reverse effects of the hypothermic storage lesion

Rejuvenation of ATP during storage does not reverse effects of the hypothermic storage lesion

2,3-Diphosphoglycerate Concentrations in Autologous Salvaged Versus Stored Red Blood Cells and in Surgical Patients After Transfusion

The effects of rejuvenation during hypothermic storage on red blood cell membrane remodeling

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