Relação entre distribuição de nitrogênio e colonização por bactérias diazotróficas em cana-de-açúcar

Gomes, Arão Araújo; Reis, Verônica Massena; Baldani, V. L. D.; Goi, Silvia Regina

doi:10.1590/s0100-204x2005001100008

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…In our study, the high number of isolates found in the stalks and leaves of sugarcane indicates that these parts of the plant can host a high bacterium diversity as also reported by Gomes et al (2005) and Suman et al (2005), which confirmed that the stalk and leaves of sugarcane present high occurrence of PGPBs. This finding is important for sugarcane because its propagation occurs through a vegetative way and, thus, other plants can disseminate these PGPBs strongly.…”

Section: Resultssupporting

confidence: 87%

Diversity of plant growth-promoting bacteria associated with sugarcane

Antunes¹,

Lyra²,

Ollero³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. The sugarcane (Saccharum spp) presents economic importance, mainly for tropical regions, being an important Brazilian commodity. However, this crop is strongly dependent on fertilizers, mainly nitrogen (N). This study assessed the plant growth-promoting bacteria (PGPB) associated with sugarcane that could be used as a potential inoculant to the crop. We evaluated the genetic diversity of PGPB in the plant tissue of sugarcane varieties (RB 867515, RB 1011, and RB 92579). The primer BOX-A1R was used to differentiate the similar isolated and further sequencing 16S rRNA ribosomal gene. The 16S rRNA gene showed the presence of seven different genera distributed into four groups, the genus Bacillus, followed by Paenibacillus (20%), Burkholderia (14%), Herbaspirillum (6%), Pseudomonas (6%), Methylobacterium (6%), and Brevibacillus (3%). The molecular characterization of endophytic isolates from sugarcane revealed a diversity of bacteria colonizing this plant, with a possible biotechnological potential to be used as inoculant and biofertilizers.

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Section: Resultssupporting

confidence: 87%

Diversity of plant growth-promoting bacteria associated with sugarcane

Antunes¹,

Lyra²,

Ollero³

et al. 2017

Genet. Mol. Res.

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“…Herbaspirillum seropedicae (BALDANI et al, 1986) and Azospirillum brasilense (TARRAND et al, 1978) can colonize roots of cereals, and their efficiency to transfer fixed nitrogen to plants was already described in rice and sugarcane (BALDANI et al, 1997, BALDANI;BALDANI, 2005, BHATTACHARJEE et al, 2008GUNRI, 2015). H. seropedicae in wheat crops can substitute nitrogen fertilization, according to a greenhouse experiment (NEIVERTH et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The beneficial effects of PGPB on plants depends on many factors, such as soil type, plant age, physiological stage and genotype, and the bacterial strain specificity (BALDANI; BALDANI, 2005, ROESCH et al, 2006. However, wheat genotypes have different capacities of association with bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…Plants considered as models, showing a positive and negative plant-bacteria interaction, were described for cultivars of rice (IR42 and IAC 4440) , sugar cane (SP70 1143 and Chunee) (GOMES et al, 2005) and wheat (CD-120 and CD104) (NEIVERTH et al, 2014). However, few studies evaluated the performance of wheat model plants inoculated with PGPB and subjected to water deficit.…”

Section: Introductionmentioning

confidence: 99%

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Plant growth-promoting bacteria effect in withstanding drought in wheat cultivars

Furlan

Saatkamp

Volpiano

et al. 2017

RSA

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Resumo -Este trabalho avaliou genótipos de trigo em condições de escassez de água inoculados com Azospirillum brasilense e Herbaspirillum seropedicae, com e sem adubação nitrogenada. Para tanto, amostras para determinação do conteúdo relativo de água na folha (CRA) e do índice de estabilidade da membrana (IEM) foram coletadas no 1º e 8º dias de uma restrição total de água no estágio de emborrachamento. Além disso, os parâmetros de biomassa, nitrogênio total (NT) e produtividade foram determinados na colheita. Como resultado, os genótipos mostraram um desempenho distinto. Os dados de CRA e IEM revelaram que a inoculação de A. brasilense e H. seropedicae são capazes de conduzir o genótipo CD 120 à tolerância à seca. Além disso, o índice de grãos foi melhorado em todas as condições em que H. seropedicae estava presente em ambos os diferentes regimes de água. O H. seropedicae inoculado juntamente com adubo nitrogenado também aumentou o rendimento de grãos sob o regime de escassez de água. Além disso, A. brasilense inoculado com adubo nitrogenado foi capaz de melhorar a massa de 1000 grãos de plantas sob escassez de água. O cultivar Frontana exibiu a capacidade de manutenção do IEM e CRA apenas quando H. seropedicae ou ambas as bactérias mais adubo nitrogenado foram aplicados, no entanto, esta cultivar não apresentou diferenças em termos de massa fresca e seca da parte aérea e radicular, NT ou produtividade. Estes resultados apontaram H. seropedicae como promissora para inoculação em cereais e o CD120 como um bom modelo de planta para estudar a interação de plantas e bactérias.Palavras-Chave -status hídrico, associação planta-bactéria, rizobactérias. Abstract -This work evaluated wheat genotypes under water deficit inoculated with Azospirillum brasilense andHerbaspirillum seropedicae, with and without nitrogen fertilization. Samples of the plants were collected to evaluate its relative water content (RWC) and membrane stability index (MSI) at the 1 st and 8 th day of total water restriction at the booting stage. The plant biomass, total nitrogen (TN) and grain yield were determined at harvesting. The genotypes showed different performances. According to the results of RWC and MSI, inoculation with A. brasilense and H. seropedicae can make the cultivar CD-120 more tolerant to drought. Grain index was improved with H. seropedicae in all conditions and water regimes. H. seropedicae with nitrogen fertilization increased grain yield under water deficit. A. brasilense with nitrogen fertilization improved the 1000-grain weight of plants under water deficit. The cultivar Frontana maintained its cellular integrity and RWC with nitrogen fertilization combined with H. seropedicae and with both bacteria, however the shoot and root fresh and dry weights, TN and yield of this cultivar showed no differences. These results show the inoculation with H. seropedicae as promising to cereals, and the cultivar CD-120 as a good plant model to study plant-bacteria interaction.

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“…Many techniques have been applied with success to monitor the presence (Oliveira et al, 2009;Silva-Froufe et al, 2009) or even quantify (Gomes et al, 2005;Munoz-Rojas and Caballero-Mellado, 2003) the inoculated bacterial strains within plants.…”

Section: Introductionmentioning

confidence: 99%

Development of a real-time PCR assay for the detection and quantification of Gluconacetobacter diazotrophicus in sugarcane grown under field conditions

Paulo¹,

Jean²,

Leona³

et al. 2014

Afr. J. Microbiol. Res.

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The objective of this study was to evaluate the viability of real-time polymerase chain reaction (PCR) to detect Gluconacetobacter diazotrophicus in sugarcane inoculated and non-inoculated with diazotrophs which are grown under field conditions. The primer pair PAL5F and PAL5R yielded a specific band of 189 bp using real time PCR with SYBER Green I. This primer pair was the most sensitive one to detect endophytic bacteria in sugarcane plants grown under field conditions and inoculated or not with bacterium. The lower limit of detection was 5 fg of template DNA, which corresponds to 12 bacterial cells. In contrast, a cultivation-dependent approach was not capable of detecting the bacteria in the same sample. The quantification of G. diazotrophicus from field grown plants using real-time PCR and a set of specific primers can be used to determine the number of bacterial cells that colonize endophytically the plant after inoculation. A highly sensitive and specific assay was developed to quantify G. diazotrophicus in sugarcane plants grown under field conditions. This assay can be used to evaluate the occurrence of the bacterium in different sample types.

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Relação entre distribuição de nitrogênio e colonização por bactérias diazotróficas em cana-de-açúcar

Cited by 9 publications

References 15 publications

Diversity of plant growth-promoting bacteria associated with sugarcane

Diversity of plant growth-promoting bacteria associated with sugarcane

Plant growth-promoting bacteria effect in withstanding drought in wheat cultivars

Development of a real-time PCR assay for the detection and quantification of Gluconacetobacter diazotrophicus in sugarcane grown under field conditions

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