Relaciones entre las abundancias del bacterioplancton y del fitoplancton en tres ecosistemas lénticos de los Andes Colombianos

Canosa, Amparo; Pinilla, Gabriel

doi:10.15517/rbt.v55i1.6064

Cited by 7 publications

(8 citation statements)

References 16 publications

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“…This is the first study in one Colombian's dam whose goal was explore the spatial variation of denitrifying communities using the nosZ functional gene that codes for nitrous oxide reductase. The values of temperature, pH and nitrate reported in the surface water at the study areas (Isla del Sol, Lozanía and Tomogó) are slightly lower than those reported previously (Canosa and Pinilla 2007, Castro-González 2014, Perea and Villanueva 2010 supporting the temporal variability recorded in the area (Guevara et al 2009). Although profiles along water column of O 2 and nutrients are very limited for this dam, the oxygen, nitrate and ammonium levels quantified during this study support the hypoxic (even suboxic) and eutrophic condition of this reservoir which has been reported several decades ago (Ducharme 1975, SODEIC 1993, Roldán 2003 The molecular analysis indicated that Lozanía contributed with a high number of sequences (3, 184), followed by Tomogó (2, 890) and Isla del Sol (623).…”

Section: Discussioncontrasting

confidence: 75%

“…The sampling was done in the Prado dam in the south west of Tolima, which is one of the biggest reservoirs in Colombia (1.254 Ha), characterized by its eutrophic conditions, physical-chemical stratification, low oxygen and high levels of organic matter and nutrient in the water column in contrast with others Colombian hydroelectric (Canosa and Pinilla 2007, Guevara et al 2009, Márquez and Guillot 2001, Perea and Villanueva 2010, Roldán 2003).…”

Section: Study Sites Sample Collection and Total Dna Extractionmentioning

confidence: 99%

“…Taking into account all these characteristics, the Prado dam is an ideal place to explore aspects about denitrifiers diversity, given its eutrophic condition, thermal stratification, and hypoxic/suboxic water column (Guevara et al 2009, Perea andVillanueva 2010). Until today, in this reservoir there have been studies about fishes Lozada 1999, 2004), zooplankton, phytoplankton (Guevara et al 2009) and bacterioplankton (Canosa and Pinilla 2007), but not related to the diversity of denitrifying microorganisms, excepting a recent study developed by Castro-González (2014) about the TRFLP diversity of nosZ-denitrifying communities between hypoxic and suboxic conditions in the water column at Isla del Sol. For this, the goal of this study was to determine and compare the composition (richness/abundance) of nosZ-type denitrifiers in three places of this dam, through PCR and 454 pyrosequencing of this functional gene.…”

Section: Introductionmentioning

confidence: 99%

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Spatial Variation of -communities in the Low Oxygen Waters of Prado Hydroelectric (South-West of Colombia)

Montealegre¹,

González²

2016

Open Ecol. J.

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Denitrification is a process of reduction of nitrate to dinitrogen by facultative anaerobic microorganisms, which have functional genes encoding denitrification enzymes (reductases). The nosZ gene encoding the enzyme that reduces nitrous oxide to dinitrogen was utilized in this research, as molecular marker for denitrifying communities into low oxygen waters of Prado reservoir. Our objective was to analyze and compare the composition (richness and abundance) of nosZ-type denitrifiers in relationship with physicochemical variables (oxygen, pH, temperature, nitrate, nitrite and ammonium) in three areas of this dam: Isla del Sol, Lozanía and Tomogó which are distant and have different anthropogenic influences. For this, we performed DNA extraction, amplification, 454 pyrosequencing and phylogenetic analysis of nosZ gene. The Chao1 estimator and Shannon index were used for compare richness and diversity of nosZ gene; and the relationship between compositions of operational taxonomic units (OTU) with physicochemical variables was established by canonical correspondence analysis (CCA). In the reservoir 45 nosZ-OTUs to species level (80% similarity) were detected. Lozanía had the highest number of OTUs (25) and greatest diversity (S CHAO1 : 35; H: 2.0), compared to the other areas sampling. The phylogenetic analysis showed the presence of many OTUs (28 of 45) with low similarity to Proteobacteria group and high similarity with sequences of environmental clones reported previously. The CCA showed that the nosZ-community composition of Prado dam was related with low pH (6.2), oxygen (0.01mg / L) and nitrate (<0.25 mg / L) recorded in the water column.

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Section: Discussioncontrasting

confidence: 75%

Section: Study Sites Sample Collection and Total Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spatial Variation of -communities in the Low Oxygen Waters of Prado Hydroelectric (South-West of Colombia)

Montealegre¹,

González²

2016

Open Ecol. J.

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“…No se realizaron difusiones en agar (Banerjee, Bandopadhyay & Shukla, 2012) por no haber sido exitosas en ensayos previos, probablemente por las elevadas densidades de microorganismos presentes en aguas marinas cálidas (Canosa & Pinilla, 2007), siendo común que las colonias de bacterias crezcan junto con las microalgas en las placas con agar (He et al, 2012). Durante el proceso de filtración realizado se retuvo el detrito y los protozoos de mayor tamaño, que se alimentan de células algales (He et al, 2012).…”

Section: Discussionunclassified

Isolation of two diatom species with aquaculture potential (Bacillariophyceae) from the Pacific of Costa Rica

Rodríguez-Núñez

Toledo

Arias

2016

URJ

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Isolation of two diatom species with aquaculture potential (Bacillariophyceae) from the Pacific of Costa Rica. Due to the importance of primary production in the trophic network, most aquatic ecosystems rely on microalgae. However, there are fewer studies on phytoplankton in the Central American region, specifically on marine diatoms, in part because it is difficult to isolate and cultivate local species in tropical climates. The objective of this study is the isolation of two local diatoms of Chaetoceros and Nitzschia, potential food for aquatic organisms. Water samples was taken from the Nicoya Gulf, located in the Central Pacific coast of Costa Rica. The samples were successively filtered first through a 35µm pore size filter and then through one of 20µm. They were repeatedly isolated using modified Pasteur pipettes, and serial dilutions were done until a monoalgal culture was obtained. Two Bacillariophyceae were isolated: NGN1, genus Nitzschia; and CGN2, genus Chaetoceros. This study is a step toward reducing the importation of phytoplankton on feeding aquatic organisms by providing technical and scientific information to develop a microalgal culture from this region.

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“…La abundancia de BOA, se correlaciona negativamente con las concentraciones de fósforo inorgánico disuelto, que podría ser explicada por posible competencia con el fitoplancton por el nutriente. Canosa & Pinilla (2007) registraron, en ecosistemas lénticos de alta montaña de Colombia, que el bacterioplancton disminuye cuando las clorofíceas aumentan, demostrando algún fenómeno de competencia entre las Tabla 2. Correlaciones significativas entre las abundancias de bacterias nitrificantes con las variables físicas y químicas del Lago de Tota, Boyacá. )…”

Section: Relación De Abundancia De Bacterias Nitrificantes (Boa Bon)unclassified

Bacterias nitrificantes cultivables de la zona limnética del lago de Tota, Boyacá

Mendoza

Forero

Riaño

2019

Rev. U.D.C.A Act. & Div. Cient.

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Palabras clave: eutrofi zación; piscifactorías; lagos de agua dulce; microbiología acuática; bacterias del ciclo del N.Másmela-Mendoza, J.E.; Lizarazo-Forero, L.M.; Aranguren Riaño, N.J.: bacterias nitrificantes lago de Tota ABSTRACT Fish production activities in Lake Tota, Boyacá are increasing concentrations of ammonium and nitrate, nutrients that are accelerating eutrophication processes. Some of the microbial communities that regulate the entry of allochthonous nitrogen are nitrifying bacteria, and the determination of their abundance in ecosystems is a measure of pollution bioindication. The objective of the study was to quantify and biochemically characterize the cultivable nitrifying bacteria of the limnetic zone of Lake Tota. The most probable number technique (MPN) was used to measure abundance. The samples were taken in the Lago Chico and Lago Grande sectors, in limnetic areas with fish activity and without intervention. Physical and chemical variables were measured in situ and the concentration of nutrients in the laboratory was determined. The highest quantifications of nitrifying bacteria were recorded in the area of influence of fish farming. The group of ammonium oxidizing bacteria (BOA) and nitrite oxidants (BON) was smaller in the central area of Lago Grande compared to fish farms in Hato Laguna and El Túnel. 14 isolates of nitrifying bacteria were obtained, genera Nitrosomonas spp. and Nitrobacter spp. Changes in BOA abundance were related to alkalinity, while BON values correlated with electrical conductivity and dissolved inorganic nitrogen. A greater abundance of nitrifying bacteria was recorded in the lake's limnetic areas affected by fish farming activity, associated with the increase in nutrients dissolved in the water column.

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Relaciones entre las abundancias del bacterioplancton y del fitoplancton en tres ecosistemas lénticos de los Andes Colombianos

Cited by 7 publications

References 16 publications

Spatial Variation of -communities in the Low Oxygen Waters of Prado Hydroelectric (South-West of Colombia)

Spatial Variation of -communities in the Low Oxygen Waters of Prado Hydroelectric (South-West of Colombia)

Isolation of two diatom species with aquaculture potential (Bacillariophyceae) from the Pacific of Costa Rica

Bacterias nitrificantes cultivables de la zona limnética del lago de Tota, Boyacá

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