Relapse of Neonatal Escherichia coli Meningitis: Did We Miss Something at First?

Vissing, Nadja Hawwa; Mønster, Mette Bondo; Nordly, Sannie; Dayani, Gholamreza K.; Heedegaard, Sofie S.; Knudsen, Jenny Dahl; Nygaard, Ulrikka

doi:10.3390/children8020126

Cited by 11 publications

(15 citation statements)

References 11 publications

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“…Therefore, the CSF samples in the remission stage were further divided into non-refractory and refractory remission, in which the corresponding disease durations were less than or greater than 28 days, respectively, during which time BM patients experienced satisfactory or unsatisfactory therapeutic effects, respectively. A recent study (45) showed that BM patients with unsatisfactory therapeutic effects presented with abnormally increased CSF TCC; in our study, some CSF of BM patients with disease durations less than 28 days showed clearly relapsed TCC values, such as C25, C40, and C97, and were classified into the "refractory remission" group. Similarly, CSF samples from BM patients in the recovery stage were divided into "recovery after non-refractory remission" or "recovery after refractory remission" groups, in which the latter group also included patients with a disease duration less than 28 days but who had recovered from a refractory condition (Figure 2A).…”

Section: Classifying Csf Samples and Their Represented Bm Onset Condi...supporting

confidence: 52%

High-throughput sequencing unravels the cell heterogeneity of cerebrospinal fluid in the bacterial meningitis of children

Xiao

Zhang

et al. 2022

Front. Immunol.

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Bacterial meningitis (BM) is a common life-threatening infection in children that occurs in the central nervous system (CNS). The cytologic examination of cerebrospinal fluid (CSF) is a key parameter in the diagnosis of BM, but the heterogeneity of cells in the CSF has not been elucidated, which limits the current understanding of BM neuroinflammation. In this study, CSF samples were collected from a number of BM patients who were in different stages of disease progression. Single-cell RNA-sequencing (scRNA-seq), with additional bulk transcriptome sequencing, was conducted to decipher the characteristics of CSF cells in BM progression. A total of 18 immune cell clusters in CSF were identified, including two neutrophils, two monocytes, one macrophage, four myeloid dendritic cells, five T cells, one natural killer cell, one B cell, one plasmacytoid dendritic cell, and one plasma cell subtype. Their population profiles and dynamics in the initial onset, remission, and recovery stages during BM progression were also characterized, which showed decreased proportions of myeloid cells and increased proportions of lymphoid cells with disease progression. One novel neutrophil subtype, FFAR2+TNFAIP6+ neutrophils, and one novel monocyte subtype, THBS1+IL1B+ monocytes, were discovered, and their quantity changes positively correlated with the intensity of the inflammatory response in the CSF during BM. In addition, the CSF of BM patients with unsatisfactory therapeutic responses presented with different cell heterogeneity compared to the CSF of BM patients with satisfactory therapeutic responses, and their CSF featured altered intercellular communications and increased proportions of type II myeloid dendritic cells and plasmacytoid dendritic cells. Moreover, the bulk transcriptome profiles of autologous CSF cells and peripheral blood leukocytes of BM patients showed that the immune cells in these two physiological compartments exhibited distinct immune responses under different onset conditions. In particular, the CSF cells showed a high expression of macrophage characteristic genes and a low expression of platelet characteristic genes compared with peripheral blood leukocytes. Our study conducted an in-depth exploration of the characteristics of CSF cells in BM progression, which provided novel insights into immune cell engagement in acute CNS infection.

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Section: Classifying Csf Samples and Their Represented Bm Onset Condi...supporting

confidence: 52%

High-throughput sequencing unravels the cell heterogeneity of cerebrospinal fluid in the bacterial meningitis of children

Xiao

Zhang

et al. 2022

Front. Immunol.

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“…coli is a representative bacterium of the genus Escherichia. Intestinal and extraintestinal infections, including urinary tract infections, arthritis, meningitis, and septicaemia, can be caused under certain conditions. − To verify this, we constructed an E. coli-induced myositis murine model.…”

Section: Resultsmentioning

confidence: 99%

Family of hNQO1 Activatable Near-Infrared Fluoro-Photoacoustic Probes for Diagnosis of Wound Infection and Ulcerative Colitis

et al. 2023

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Bacterial infections can easily occur when patients mishandle wounds or eat moldy food. The prompt diagnosis of a bacterial infection could effectively reduce the risk of possible anatomical damage. However, non-invasive early detection of bacterial infections is difficult to achieve due to the lack of favorable tools. Here, we designed two hNQO1 fluorescent probes (RX2 and RX3) to visualize bacterial infection after deep learning on the pathogenesis of bacterial infection. RX2 and RX3 enable early detection of bacterial infection and are verified to be, respectively, suitable for fluorescence imaging (FLI) and photoacoustic imaging (PAI) by comparing the signal-to-background ratio of both probes in a mouse model of myositis caused by Escherichia coli infection. In view of the difference in penetration depth between the two imaging modalities, we further applied RX2 for FLI of E. coli-infected wounds and RX3 for PAI of E. coli-infected inflammatory bowel disease, suggesting the great potential of both probes for early diagnosis of bacterial infections.

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“…The ExPEC pathotype is divided into six main sub-pathotypes [50,58], including uropathogenic E. coli (UPEC) [88][89][90][91][92], sepsis/newborn meningitis-associated E. coli (NMEC) [93][94][95][96], APEC [45,[97][98][99][100][101][102], sepsis-associated pathogenic E. coli (SePEC) [103,104], mammary pathogenic E. coli [105][106][107][108], and endometrial pathogenic E. coli [109,110]. The ExPEC strain was characterized as E. coli isolate containing two or more virulence factors.…”

Section: Avian Pathogenic E Colimentioning

confidence: 99%

Occurrence, antimicrobial resistance, and potential zoonosis risk of avian pathogenic Escherichia coli in Indonesia: A review

2022

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Avian pathogenic Escherichia coli (APEC) causes avian colibacillosis or colibacillosis and is a major endemic disease of poultry worldwide, including in Indonesia. It is characterized by a black proventriculus and can damage other organs, leading to pericarditis, perihepatitis, water sacculitis, mesenteritis, and omphalitis. The APEC strain is one of the six main sub-pathotypes of the extraintestinal pathogenic E. coli (ExPEC) pathotype. The relationship between APEC and infection in humans is questionable. The previous studies have suggested poultry products, including meat and eggs, as a potential source of infection for the transmission of ExPEC disease to humans. Due to the absence of reporting of disease incidents and the lack of literature updates on this disease, it seems as if APEC does not exist in Indonesia. Since bacterial resistance is a growing problem in Indonesia, and globally, the World Health Organization issued a statement regarding the importance of assessing related factors and their control strategies. Antimicrobial resistance, especially multidrug resistance, presents a challenge when treating infectious diseases. In Indonesia, the incidence of resistance to several antimicrobials in cases of avian colibacillosis is high. In addition, avian and human extraintestinal E. coli infections present a potential zoonotic risk. Furthermore, a relationship exists between antibiotic resistance to foodborne bacteria and the occurrence of antibiotic resistance in humans, so the use of antibiotics in the poultry industry must be controlled. Therefore, the One Health strategy should be implemented to prevent the overuse or misuse of antibiotics in the poultry industry. This review aimed to increase awareness of people who are at risk of getting Avian pathogenic Escherichia coli (APEC) from poultry by controlling the spread of APEC by maintaining a clean environment and hygienic personnel in poultry farms.

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Relapse of Neonatal Escherichia coli Meningitis: Did We Miss Something at First?

Cited by 11 publications

References 11 publications

High-throughput sequencing unravels the cell heterogeneity of cerebrospinal fluid in the bacterial meningitis of children

High-throughput sequencing unravels the cell heterogeneity of cerebrospinal fluid in the bacterial meningitis of children

Family of hNQO1 Activatable Near-Infrared Fluoro-Photoacoustic Probes for Diagnosis of Wound Infection and Ulcerative Colitis

Occurrence, antimicrobial resistance, and potential zoonosis risk of avian pathogenic Escherichia coli in Indonesia: A review

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