Relating microfeatures of soil organic matter to C stabilisation: optical microscopy, SEM-EDS, abiotic oxidation

Falsone, Gloria; Wilson, Clare; Cloy, Joanna M.; Graham, Margaret C.; Bonifacio, Eleonora

doi:10.1007/s00374-013-0883-6

Cited by 9 publications

(8 citation statements)

References 33 publications

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“…Consequently, we expected that OM quality, evaluated as relative C and N contents, was important in driving the proportion of OCrec and OClab. In fact, the C/N ratio is often taken as index of OM quality and was found to be related to the sensitivity to oxidation treatments (Falsone et al, 2014). In spite of this, our results showed that the relationships between CUT/N ratios and the proportions of OClab (n=79, r=-0.305, P<0.01) and OCrec (n=79, r=0.366, P<0.01) were rather poor, as the determination coefficients explained only 9 and 13% of sample variability, respectively.…”

Section: Discussionmentioning

confidence: 99%

Effect of pedogenic processes and formation factors on organic matter stabilization in alpine forest soils

et al. 2016

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Understanding the mechanisms involved in the building up of organic matter pools with long residence time in soils is fundamental for the comprehension of carbon dynamics. As organic matter persistence depends on environmental conditions and soil properties, both pedogenic processes and factors should be key elements for identifying the link between organic pools and stabilization mechanisms.In the Ligurian Alps, an area where pedodiversity is large, we evaluated the importance of two soil formation factors, vegetation and parent material, and consequently pedogenic processes and soil features, on organic matter stabilization in forest soils. We thus selected 20 profiles, developed on different parent materials and forest stands, which ranged from Regosols, to Cambisols, to Chernozems/Kastonozems, to Podzols and Luvisols/Alisols. In the mineral horizons, we determined the soil physico-chemical properties and fractionated organic matter into labile and stabilized (recalcitrant and mineral-bound) pools using NaClO followed by HF treatment.Soil and organic matter characteristics were primarily explained by the parent material and soil type, while vegetation affected organic matter quality but not the total amounts. The labile and recalcitrant organic pool proportions were linked to parent material likely through its effect on pH and other microenvironment characteristics. The mineral-associated organic matter instead followed a pedogenic pathway, with higher percentages of stable organic carbon in illuvial B horizons from Podzols and Luvisols/Alisols. In the study area, therefore, the most developed soils were relatively more resilient for organic matter conservation than less developed soils, which are more prone to C losses.

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Section: Discussionmentioning

confidence: 99%

Effect of pedogenic processes and formation factors on organic matter stabilization in alpine forest soils

et al. 2016

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“…Mosaics created with sequential images taken from a microscope were proposed as a solution to the spatial resolution and to increase the area of the soil thin section studied; however, they were constructed from a small area only of the thin section (Adderley et al, 2002;Falsone et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Thematic micro‐maps of soil components using high‐resolution spatially referenced mosaics from whole soil thin sections and image analysis

Gutiérrez-Castorena

González-Vargas

et al. 2017

European J Soil Science

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Summary In the quantification and analysis of the distribution of soil basic components, factors such as spatial resolution, scales of observation and analytical techniques used to interpret digital images of microscopic platforms are critical to obtain reproducible results. In addition, individual or discrete images, sometimes with high spatial resolution, distort features or basic components when zooming in or out, which limits their classification. This research proposes the use of micromorphological tools, spatially referenced mosaics with high resolution, image analysis and composite images to elaborate thematic maps of soil components. Therefore, we created mosaics with sequential digital images from soil thin sections (up to 3850 mm2) of different diagnostic horizons, with different magnifications (2×, 10× and 20×) and light sources. The mosaics (between 0.25 and 2.6 μm2 per pixel, and between 2 and 8 GB file size) were processed by image analysis (segmentation, supervised classification and accuracy assessments) for delimiting their basic soil components from composite images and with spatial operators to elaborate thematic maps at the microscopic level. The RGB (red, green, blue) brightness values of each soil component with different light sources enabled us to identify and quantify size classes of aggregates, voids, pedofeatures and organic matter with different degrees of decomposition at a high level of precision (up to 99% overall accuracy). It was also possible to develop good‐quality thematic maps of such components from high‐resolution spatially referenced mosaics and composite images Thematic maps of soil components can be explored without losing their spatial reference and analysed on multiple scales from a whole soil thin section. Highlights Micromorphology and digital cartography to develop thematic maps of soil components High spatial resolution mosaics from thin sections and image analysis were used for mapping RGB brightness values of different light sources facilitated mapping of soil features Accurate maps enabled quantification and analysis of spatial relations of soil components

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“…To measure pores, multiple images of the same representative aggregates were taken under both plane (PPL; Figure S1-a) and crossed polarized light (XPL) at 0.5 and 15° (Falsone et al, 2014). This was necessary to distinguish between pores and quartz, since both were translucent under PPL.…”

Section: Total Porosity and Pore Size Distributionmentioning

confidence: 99%

“…In this work we took into account the Al:C, Fe:C and Ca:C molar ratios as indicators of the degree of organicmetals/minerals interactions (Falsone et al, 2014).…”

Section: Sem-eds Analysis On the Aggregate Thin Sectionsmentioning

confidence: 99%

New insights into organic carbon stabilization in soil macroaggregates: An in situ study by optical microscopy and SEM-EDS technique

et al. 2021

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Relating microfeatures of soil organic matter to C stabilisation: optical microscopy, SEM-EDS, abiotic oxidation

Abstract: 1We investigated the relationships between microscale distribution of soil organic matter 2 (SOM) features and their stability by combining optical microscopy, SEM-EDS analysis and 3

Cited by 9 publications

References 33 publications

Effect of pedogenic processes and formation factors on organic matter stabilization in alpine forest soils

Effect of pedogenic processes and formation factors on organic matter stabilization in alpine forest soils

Thematic micro‐maps of soil components using high‐resolution spatially referenced mosaics from whole soil thin sections and image analysis

New insights into organic carbon stabilization in soil macroaggregates: An in situ study by optical microscopy and SEM-EDS technique

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