Public transport systems have a vital role in achieving sustainable mobility goals, diminishing reliance on private individual transport and improving overall public health. Despite that, transport operators are often in situations that require them to cope with complex working conditions that lead to negative emotions such as anger. The current study represents a segment of the permanent global research agenda that seeks to devise and test a psychometric scale for measuring driving anger in professional drivers. The present research is one of the first attempts to examine the factorial validity and the cross-cultural measurement equivalence of the broadly utilized Driving Anger Scale (DAS) in three culturally different countries within the Western Balkans region. The respondents (N = 1054) were taxi, bus and truck drivers between 19 and 75 years of age. The results pertaining to confirmatory factor analysis showed that there were adequate fit statistics for the specified six-dimensional measurement model of the DAS. The measurement invariance testing showed that the meaning and psychometric performance of driving anger and its facets are equivalent across countries and types of professional drivers. Furthermore, the results showed that driving anger facets had positive correlations with dysfunctional ways of expressing anger and negative correlations with the form of the prosocial anger expression. In addition, the results revealed that taxi drivers displayed considerably higher levels of anger while driving and aggressive driving than truck and bus drivers. Overall, this study replicates and extends the accumulated knowledge of previous investigations, suggesting that the original DAS remains a reliable and stable instrument for measuring driving anger in day-to-day driving conditions.