Relating the Biogenesis and Function of P Bodies in Drosophila to Human Disease

Wilby, Elise L.; Weil, Timothy T.

doi:10.3390/genes14091675

Cited by 4 publications

(1 citation statement)

References 194 publications

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“…Processing bodies are thought to be sites of RNA metabolism within the cytoplasm of cells. Pbodies have been implicated in mRNA decay, translation repression, nonsense mediated decay (NMD), as well as mRNA silencing [76,[92][93][94][95][96][97]. They have commonly been identified as sites of mRNA decay due several constituents' ties to transcript degradation, namely DDX6, DCP1, DCP2, Xrn1, EDC3, EDC4, among others [69][70][71][72][73][74][75][76][77][78][79][80].…”

Section: Viral Factors Exercise Control Over P-body Rbps To Promote V...mentioning

confidence: 99%

A Balancing Act: The Viral-Host Battle over RBPs

Bermudez,

Hatfield,

Muller

2023

Preprint

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A defining feature of a productive infection is the co-opting of host cell resources for viral replication. Despite their repertoire of molecular functions, viruses subvert host defenses to take control of cellular factors such as RNA binding proteins (RBPs). RBPs are involved in virtually all steps of mRNA life forming ribonucleoprotein complexes (mRNPs) in a highly ordered and regulated process to control RNA fate and stability in the cell. Thus, the hallmark of this viral takeover is to reshape RNA fate in the cell to modulate host gene expression and evade immune responses. Here we provide an extensive review of work in this area, particularly how the host-viral interplay influences RBP functions to modulate the host cell. Overall, in this review, we highlight the myriad of ways RBPs can regulate RNA stability in either a pro-viral or antiviral manner by gathering novel insights gained from research studies in this field.

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Section: Viral Factors Exercise Control Over P-body Rbps To Promote V...mentioning

confidence: 99%

A Balancing Act: The Viral-Host Battle over RBPs

Bermudez,

Hatfield,

Muller

2023

Preprint

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The role of ER exit sites in maintaining P-body organization and integrity during Drosophila melanogaster oogenesis

Milano,

Bayer,

et al. 2024

EMBO Rep

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Processing bodies (P-bodies) are cytoplasmic membrane-less organelles which host multiple mRNA processing events. While the fundamental principles of P-body organization are beginning to be elucidated in vitro, a nuanced understanding of how their assembly is regulated in vivo remains elusive. Here, we investigate the potential link between ER exit sites and P-bodies in Drosophila melanogaster egg chambers. Employing a combination of live and super-resolution imaging, we find that P-bodies associated with ER exit sites are larger and less mobile than cytoplasmic P-bodies, indicating that they constitute a distinct class of P-bodies. Moreover, we demonstrate that altering the composition of ER exit sites has differential effects on core P-body proteins (Me31B, Cup, and Trailer Hitch), suggesting a potential role for ER exit sites in P-body organization. Furthermore, we show that in the absence of ER exit sites, P-body integrity is compromised and the stability and translational repression efficiency of the maternal mRNA, oskar, are reduced. Together, our data highlights the crucial role of ER exit sites in governing P-body organization.

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The mRNA dynamics underpinning translational control mechanisms of Drosophila melanogaster oogenesis

Bayer,

Milano,

Bratu

2024

Biochemical Society Transactions

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Advances in the study of mRNAs have yielded major new insights into post-transcriptional control of gene expression. Focus on the spatial regulation of mRNAs in highly polarized cells has demonstrated that mRNAs translocate through cells as mRNA:protein granules (mRNPs). These complex self-assemblies containing nuclear and cytoplasmic proteins are fundamental to the coordinated translation throughout cellular development. Initial studies on translational control necessitated fixed tissue, but the last 30 years have sparked innovative live-cell studies in several cell types to deliver a far more nuanced picture of how mRNA-protein dynamics exert translational control. In this review, we weave together the events that underpin mRNA processes and showcase the pivotal studies that revealed how a multitude of protein factors engage with a transcript. We highlight a mRNA's ability to act as a ‘super scaffold’ to facilitate molecular condensate formation and further moderate translational control. We focus on the Drosophila melanogaster germline due to the extensive post-transcriptional regulation occurring during early oogenesis. The complexity of the spatio-temporal expression of maternal transcripts in egg chambers allows for the exploration of a wide range of mechanisms that are crucial to the life cycle of mRNAs.

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Relating the Biogenesis and Function of P Bodies in Drosophila to Human Disease

Cited by 4 publications

References 194 publications

A Balancing Act: The Viral-Host Battle over RBPs

A Balancing Act: The Viral-Host Battle over RBPs

The role of ER exit sites in maintaining P-body organization and integrity during Drosophila melanogaster oogenesis

The mRNA dynamics underpinning translational control mechanisms of Drosophila melanogaster oogenesis

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