Relation Between Basophilia and Fine Structure of Cytoplasm in the Fungus <i>Allomyces macrogynus</i> Em

Blondel, Benigna; Turian, G.

doi:10.1083/jcb.7.1.127

Cited by 97 publications

(33 citation statements)

References 33 publications

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“…It was, therefore, necessary to study the morphology of the hyphal cells and sclerotia of S. rolfsii at the submicroscopic level before attempting to correlate any changes in the cell organelles with changes in partial pressures of oxygen and carbon dioxide_ Besides describing the salient morphological features, this investigation also includes a cytochemical study of the concentric lamellar structures observed in the hyphae of this fungus. Although similar structures have been observed in other fungi (Blondel and Turian 1960;Hyde and Walkinshaw 1966;Ishihara and Iwaya 1966), considerable uncertainty still exists about their function. Because of the close similarity of the lamellar structures ("fungal mesosomes") to bacterial mesosomes, an investigation was made to determine whether these were homologous structures.…”

Section: Introductionsupporting

confidence: 55%

“…This was further confirmed by demonstrating that these lamellae or mesosome-like structures did not serve as sites of oxidativereductive events. The results obtained by using potassium tellurite probably indicate that the "fungal mesosomes" or mesosome-like structures described in S. rolfsii and other fungi like Allomyces macrogynous (Blondel and Turian 1960), Microsporum gypseum (Ishihara and Iwaya 1966), and Lenzites saepiaria (Hyde and Walkinshaw 1966) are not homologous with bacterial meso somes, but serve other functions such as formation of lomasomes as in S. rolfsii or may even be products of metabolism as in Allomyces macrogynous (Blondel and Turian 1960).…”

Section: Discussionmentioning

confidence: 99%

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Fine Structure and Electron Cytochemical Studies of Sclerotium Rolfsii Sacc

Nair¹,

White²,

Griffin³

et al. 1969

Aust. Jnl. Of Bio. Sci.

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SummaryA characteristic feature of the hyphal cells of S. rolfsii is an electron-dense sheath surrounding the cell wall. The cell sheath could act as a barrier to diffusion of gases. The septal pore apparatus in S. rolfsii conforms to the general pattern of dolipore septum in other basidiomycetes, and appears to be well adapted to protoplasmic streaming and subsequent plugging of the septal pore. A prominent feature of S. rolfsii is the presence of concentric and parallel lamellar structures in the hyphal cells. We believe that lomasomes originate through a process of budding of the concentric lamellae into tubular vesicles. Though these lamellar structures resemble bacterial mesosomes, these two organelles do not appear to be homologous structures. Through cytochemical tests we have been able to show that oxidative-reductive events are not located in these lamellae but are integrated in the mitochondria of this fungus. The presence of reduced tellurite in the endoplasm probably indicates that oxidative-reductive events are distributed in the cell.

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Section: Introductionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Fine Structure and Electron Cytochemical Studies of Sclerotium Rolfsii Sacc

Nair¹,

White²,

Griffin³

et al. 1969

Aust. Jnl. Of Bio. Sci.

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“…After about 2 days, many of the hyphal tips showed different stages of gametangial differentiation. The culture was then fixed for an hour at 5°C with 1 per cent OsO4 buffered to pH 6.1 with 0.2 M phosphate buffer and containing 10 -3 M CaC12 (Blondel and Turian, 1960). Except for the study of hyphal tips, it was found necessary to treat the organism, prior to fixation, with a crude chitinase preparation (Bawden and Pirie, 1946;Neuberger and Pitt Rivers, 1939), to improve the penetration of the fixative through to the thick gametangial wall.…”

Section: Allomyces Arbusculus Butler Was Obtained From Thementioning

confidence: 99%

THE DEVELOPMENT OF BASAL BODIES AND FLAGELLA IN ALLOMYCES ARBUSCULUS

Renaud

Swift

1964

The Journal of Cell Biology

142

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The development of basal bodies and flagella in the water mold Allomyces arbusculus has been studied with the electron microscope. A small pre-existing centriole, about 160 mµ in length, was found in an inpocketing of the nuclear membrane in the vegetative hypha. Thus, formation of a basal body does not occur de novo. When the hyphal tip started to differentiate into gametangia, the centrioles were found to exist in pairs. One of the members of the pair then grew distally to more than three times its original length, whereas the other remained the same size. The larger centriole would correspond to the basal body of a future gamete. Gametogenesis was usually induced by transferring a "ripe" culture to distilled water. Shortly after this was done, a few vesicles were pinched off from the cell membrane of the gametangium and came in contact with the basal body. Apparently, they fused and formed a large primary vesicle. The flagellum then started to grow by invaginating into it. Flagellar fibers were evident from the very beginning. As the flagellum grew so did the vesicle by fusion with secondary vesicles, thus coming to form the flagellar sheath. The different stages of flagellar morphogenesis are described and the possible interrelationships with other processes are discussed.

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“…6, fig. 16) ; clearly, these should not be confused with the lobed osmiophilic granules of different shape found in the cytoplasm of Allomyces (Blonde1 & Turian, 1960). We believe that they consist of a spherical body incompletely girdled by a band of strongly osmiophilic material.…”

Section: The Cytoplasm and Inclusionsmentioning

confidence: 89%

The Single Mitochondrion, Fine Structure, and Germination of the Spore of Blastocladiella emersonii

Cantino

Lovett

Leak

et al. 1963

Journal of General Microbiology

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SUMMARYThe motile spore of the water fungus, BlatocladkZZu emersmii, contains a single, large, posterior, eccentrically disposed mitochondrion; some 6-12 prominent, strongly osmiophilic, lipid-like organelles, bordered by a double membrane, occur along its outer edge. A single flagellum with the classical 9-plus-2 fibrillar structure is attached by at least one banded rootlet to the mitochondrion. The nuclear cap (a package of ribosomes) overlies the nucleus and is separated from it by a double membrane. The cytoplasm is somewhat granular, contains structures believed to be organelles (previously described as y particles), but is devoid of any obvious cytoplasmic reticulum. Before spore germination, the flagellum undergoes a series of characteristic movements. Following this, the nuclear apparatus rotates through some 270°, but the spore itself does not turn. The flagellum is then retracted into the cell. Subsequently, the spore germinates by formation of a germ tube and the nuclear cap disintegrates.

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Relation Between Basophilia and Fine Structure of Cytoplasm in the Fungus Allomyces macrogynus Em

Cited by 97 publications

References 33 publications

Fine Structure and Electron Cytochemical Studies of Sclerotium Rolfsii Sacc

Fine Structure and Electron Cytochemical Studies of Sclerotium Rolfsii Sacc

THE DEVELOPMENT OF BASAL BODIES AND FLAGELLA IN ALLOMYCES ARBUSCULUS

The Single Mitochondrion, Fine Structure, and Germination of the Spore of Blastocladiella emersonii

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