Relation between fluid viscosity and compressibility

Atanov, Yu. A.; Berdenikov, A. I.

doi:10.1007/bf00825016

Cited by 1 publication

(1 citation statement)

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“…This phenomenon has been described more generally in the literature, with multiple studies showing that when lysis occurs, the cellular content perfuses into the surrounding liquid and causes an increase in viscosity [16,17,18,19]. There is an empirical dependence between viscosity, density, and compressibility of a liquid [20,21], and more specifically to acoustic trapping applications, groups have shown that changes in viscosity of the surrounding fluid impacts the acoustic radiation force experienced by particles in a standing wave [22,23]. With a proper understanding of the relationship between these parameters, and the knowledge that cellular lysis and variation among samples will impact fluid properties, we assume that significant changes in density and compressibility due to sample characteristics will adversely affect the ability to trap sperm cells due to a shift in optimal resonant frequency.…”

Section: Introductionmentioning

confidence: 99%

Real Time Electronic Feedback for Improved Acoustic Trapping of Micron-Scale Particles

et al. 2019

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Acoustic differential extraction has been previously reported as a viable alternative to the repetitive manual pipetting and centrifugation steps for isolating sperm cells from female epithelial cells in sexual assault sample evidence. However, the efficiency of sperm cell isolation can be compromised in samples containing an extremely large number of epithelial cells. When highly concentrated samples are lysed, changes to the physicochemical nature of the medium surrounding the cells impacts the acoustic frequency needed for optimal trapping. Previous work has demonstrated successful, automated adjustment of acoustic frequency to account for changes in temperature and buffer properties in various samples. Here we show that, during acoustic trapping, real-time monitoring of voltage measurements across the piezoelectric transducer correlates with sample-dependent changes in the medium. This is achieved with a wideband peak detector circuit, which identifies the resonant frequency with minimal disruption to the applied voltage. We further demonstrate that immediate, corresponding adjustments to acoustic trapping frequency provides retention of sperm cells from high epithelial cell-containing mock sexual assault samples.

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