Relation between in vitro and in vivo assessment of amino acid availability

Galibois, Isabelle; Savoie, L.; Nunes, C. Simões; Rérat, A.

doi:10.1051/rnd:19890411

Cited by 9 publications

(7 citation statements)

References 8 publications

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“…These fractions only need ultimate degradation by the brush‐border peptidases before being absorbed as free amino acids or as di‐ or tripeptides 16. This hypothesis is supported by the close correlation between the amino acid pattern in the peptides released and dialysed during in vitro digestion and the plasma amino acids of the intestinal effluent in rats and pigs 18, 19…”

Section: Discussionmentioning

confidence: 83%

Kinetics of peptide fraction release during in vitro digestion of casein

et al. 2004

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The charges and sizes of peptides released during in vitro casein digestion were investigated. Casein was hydrolysed sequentially by pepsin and pancreatin and, during hydrolysis, the products of digestion were removed by dialysis. The undigested residues were separated by ion-exchange chromatography into basic-neutral (BN), lightly acid (LA) and acid (A) fractions. Each of these fractions was further resolved by sequential ultrafiltration (MWCO 10 and 1 kDa) into two retentates (>10 and 10-1 kDa) and one permeate (<1 kDa). The polypeptides (>10 kDa) produced by pepsin hydrolysis were degraded into small molecules during the first 2 h of pancreatin hydrolysis; less than 100 mg g −1 of the total N remained undigested. Most of the material produced was in the BN10-1 (ie basic-neutral, 10-1 kDa) fraction, with three times as much N as the A10-1 fractions. As digestion progressed a decrease in the proportion of N in the residues retained by the dialysis membrane was observed. This decrease was particularly rapid in the BN10-1 fraction. Large proportions of leucine (Leu), lysine (Lys), arginine (Arg), phenylalanine (Phe) and tyrosine (Tyr) were found as peptides smaller than 1 kDa, both in the dialysates and retentates, while glutamine (Glu), threonine (Thr), serine (Ser) and asparagine (Asp) appeared mostly in the A10-1 and A >10 fractions. After 6 h of pancreatin hydrolysis most of the proline (Pro) content was in the BN10-1 fractions. The mechanisms behind and the implications of these results are discussed.

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Section: Discussionmentioning

confidence: 83%

Kinetics of peptide fraction release during in vitro digestion of casein

et al. 2004

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“…The influence of the time factor was investigated by Galibois et al (1989) by comparing the essential amino acid (EAA) profiles in the dialysate collected at 3 h intervals during 24 h of in vitro enzymic proteolysis of casein and rapeseed protein with the pattern of appearance of dietary EAA in the portal vein of pigs fed on the same proteins. The results demonstrated a close relationship between the in vitro release of amino acids and the appearance of dietary EAA in the portal vein.…”

Section: Gauthiermentioning

confidence: 99%

Critical Evaluation of in Vitro Methods for Estimating Digestibility in Simple-Stomach Animals

1991

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“…-In vivo testing of the accuracy of an in vitro technique used to forecast amino acid availability [64].…”

Section: Absorption Of Minerals Sugars and Amino Acidsmentioning

confidence: 99%

Forty years of achievement in french research on digestive physiology in the pig

Laplace¹,

Aumaı̂tre²,

Rérat³

2001

Reprod. Nutr. Dev.

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-The contribution of the research group set up by Rérat in the early 1960s in Jouy-en-Josas, and then expanded to Rennes, is summarised. As digestive processes are a major key to the nutritional status of monogastric animals, original methodologies based on advanced experimental surgery were developed in the pig to quantify the digestion and absorption yield, to know their factors of variation, and to understand their physiological and nutritional consequences. The group also extended its expertise to the control of food intake, the role of the nervous system and regulatory peptides, and several biomedical gut-related topics. pig / digestion / absorption / neurohumoral regulation / adaptationRésumé -Réalisations et succès de la recherche française : quarante ans de physiologie digestive chez le porc. Dès le début des années 60, un groupe de recherches a été constitué à l'initiative de A. Rérat à Jouy-en-Josas pour étudier divers aspects de la physiologie digestive chez le porc. Leur exploration a fourni des clés essentielles pour la maîtrise du statut nutritionnel chez cette espèce monogastrique, et un instrument d'amélioration de la production porcine. Ce groupe s'est élargi au fil des années et a même essaimé dans les années 80, notamment à Rennes. Il a apporté une contribution essentielle par le développement de méthodologies nouvelles généralement basées sur une chirurgie expérimentale très élaborée. Ces approches ont fourni de nombreux travaux originaux qui ont permis de décrire les processus de digestion (motricité, transit, sécrétions, hydrolyses enzymatiques

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Relation between in vitro and in vivo assessment of amino acid availability

Cited by 9 publications

References 8 publications

Kinetics of peptide fraction release during in vitro digestion of casein

Kinetics of peptide fraction release during in vitro digestion of casein

Critical Evaluation of in Vitro Methods for Estimating Digestibility in Simple-Stomach Animals

Forty years of achievement in french research on digestive physiology in the pig

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