Relation Between Platelet Integrity and Sulfhydryl Groups.

Koppel, J. L.; Novák, L; Olwin, John H.

doi:10.3181/00379727-100-24581

Cited by 6 publications

(3 citation statements)

References 1 publication

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“…Mersalyl inhibits the ATPase activity of isolated thrombosthenin (Nachman et al 1967, Zucker-Franklin et al 1967) and is a powerful inhibitor of superprecipitation of thrombosthenin Liischer 1965). Mersalyl inhibits the 'ecto-ATPase' of platelets (Chambers et al 1967) and the break-down of washed platelets is accelerated (Koppel et al 1959). A concentration of 10-~-10-~ M does not inhibit pseudopod formation or spreading of platelets (Braunsteiner et al 1960).…”

Section: Mersalymentioning

confidence: 95%

Effects of Some Chemicals on Blood Platelet Microtubules, Platelet Shape and Some Platelet Functions in vitro

Behnke

1970

Scandinavian Journal of Haematology

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Human blood platelets were treated in vitro with various chemicals to test whether a chemical which affects microtubules (demecolcine) also affects platelet shape and platelet function (aggregation and contraction) and whether chemicals which affect platelet function (cyanide, monoidoacetate, cyanide plus monoiodoacetate, fluoride, Ouabain, N-ethylmaleimide, Mersalyl, and cocaine) also affect platelet shape and platelet microtubules. It is concluded that an intact marginal bundle of microtubules is essential for the maintenance of platelet shape but intact microtubules are not essential for ADPinduced platelet aggregation or for clot retraction.

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Section: Mersalymentioning

confidence: 95%

Effects of Some Chemicals on Blood Platelet Microtubules, Platelet Shape and Some Platelet Functions in vitro

Behnke

1970

Scandinavian Journal of Haematology

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“…This strongly sugpests that the action of the inhibitors is on the datelets. An increase in nlatolet breakdown on incubation with siilfhvdrvl inhihitors has been shown previoiiclv (4). With the short incilbation periods 'of datelets with inhibitors wed in oiir exnerimants.…”

Section: Eflect Of Pcmb Nem and Mmn On Platelet Agglutination Inducementioning

confidence: 96%

“…The means by which these substances induce agglutination, and the relationship of agglutination to alterations in enzymatic activities of platelets are controversial. Through the use of sulfhydryl reagents and inhibitors, intact thiol groups have been demonstrated to be essential for maintenance of platelet integrity (3,4) and for certain enzymatic and biological functions of platelets ( 5,6,7). However, the role of thiol groups in platelet agglutination is not clear.…”

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confidence: 99%

Effect of Sulfhydryl Inhibitors on Platelet Agglutinability.

Robinson

Mason

Wagner

1963

Experimental Biology and Medicine

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Thrombin and adenosine diphosphate (ADP) induce platelet agglutination in the presence of appropriate divalent cations( 1, 2). The means by which these substances induce agglutination, and the relationship of agglutination to alterations in enzymatic activities of platelets are controversial. Through the use of sulfhydryl reagents and inhibitors, intact thiol groups have been demonstrated to be essential for maintenance of platelet integrity(3,4) and for certain enzymatic and biological functions of platelets( 5,6,7). However, the role of thiol groups in platelet agglutination is not clear. Grette found no inhibition of platelet viscous metamorphosis with the mexaptide forming agent Mersalyl at 35°C. With lower temperatures, a delay in the appearance of platelet aggregation was observed with Mersalyl ( 7). Zucker and Borrelli reported that mercuric chloride inhibited the thrombin-produced viscous metamorphosis of washed platelets (8). In platelet-rich plasma, platelet aggregation induced by thrombin or ADP was inhibited by 2.5 X 103 M mercuric chloride, but not by 2.5 X 10" M mercuric chloride or the thiol alkylating agent iodoacetate (9). The present study describes alterations in the ability of platelets to agglutinate with thrombin and ADP, in the presence of calcium, when platelets are incubated with certain sulfhydryl inhibitors.Materials and methods. Adenosine diphosphate, N-ethyl maleimide (NEM), and the sodium salt of p-chloromercuribenzoic acid (PCMB) were acquired from Mann Research Laboratories. Methyl mercuric nitrate (MMN) was prepared from recrystallized methyl mercuric iodide (Columbia Organic Chemicals Co.) by equimolar reaction with silver nitrate in methanol. The silver iodide which formed was removed by filtration and the product was crystallized twice from * This investigation was supported in part by a research grant from Nat. Inst. Health, P.H.S. methanol. Tris buffer, 0.05 M tris-hydroxymethyl aminomethane, pH 7.4, was made isotonic with sodium chloride. I t was used to prepare all inhibitor solutions and for making 22 mM solutions of CaClz and MgC12 from 0.22 M aqueous cation solutions. The solvent for ADP and thrombin was 0.154 M NaC1. Topical thrombin (Parke Davis & Co.) was partially purified on a G-25 Sephadex column equilibrated with normal saline. Thrombin unitage is expressed in Iowa units ( 10). Standard suspensions of washed canine platelets were prepared at 4OC from canine plasma collected in isotonic, pH 7.4, ethylenediamine tetra-acetic acid ( 1 1 ) . The washed platelets were suspended at a concentration of 400,00O/cmm in a solution of 0.0054 M sodium citrate and 0.146 M NaCl at 4OC. Platelet suspensions were kept in an ice bath and used within 4 hours of preparation. Only platelet preparations which gave 4+ agglutination with both thrombin (1.0 U / d , final concentration) and ADP were used. All glassware was silicone-coated (G. E. Dri-Film SC-87) except the test tubes in which agglutination tests were performed.The macroscopic platelet agglutination test of Brinkhous et aL(1...

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Cytochemical observations of hemolymph cells during coagulation in the crayfish, orconectes virilis

Wood

Podlewski

Shenk

1971

Journal of Morphology

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Smears of Orconectes virilis hemolymph were prepared by fixation in 10% formalin immediately upon withdrawal, and after an interval of 1, 5, or 15 minutes. These smears were then stained by a variety of histochemical means designed to identify a material which is released from both hyaline and granular cells during the coagulation process. Based upon reactions to various carbohydrate, lipid and protein tests, this material appears to consist of a glycoor mucwprotein, and its localization and activity coincide with the "fibrin ferment" described by Hardy (1892). Its release is probably initiated from the hyaline cells, followed shortly thereafter by a similar activity of the granulocytes. While it cannot be ascertained by the methods used in this study whether or not this material participates actively in clotting, its release is nevertheless coincidental with coagulation.

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Relation Between Platelet Integrity and Sulfhydryl Groups.

Cited by 6 publications

References 1 publication

Effects of Some Chemicals on Blood Platelet Microtubules, Platelet Shape and Some Platelet Functions in vitro

Effects of Some Chemicals on Blood Platelet Microtubules, Platelet Shape and Some Platelet Functions in vitro

Effect of Sulfhydryl Inhibitors on Platelet Agglutinability.

Cytochemical observations of hemolymph cells during coagulation in the crayfish, orconectes virilis

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